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Oral Biology

Molecular Techniques and Applications

  • Book
  • © 2023

Overview

Editors:
  1. Gregory J. Seymour

    1. School of Dentistry, The University of Queensland, Herston, Australia

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  2. Mary P. Cullinan

    1. School of Dentistry, The University of Queensland, Herston, Australia

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  3. Nicholas C.K. Heng

    1. Faculty of Dentistry, University of Otago, Dunedin, New Zealand

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    You can also search for this editor in PubMed   Google Scholar

  4. Paul R. Cooper

    1. Faculty of Dentistry, University of Otago, Dunedin, New Zealand

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2588)

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Table of contents (32 protocols)

  1. Front Matter

    Pages i-xv
    Download chapter PDF

  2. Saliva and Other Oral Fluids

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. RNA Sequencing Analysis of Saliva exRNA

      • Karolina Elżbieta Kaczor-Urbanowicz, David T. W. Wong
      Pages 3-11

    3. Proteome Analysis of Oral Biofluids in Periodontal Health and Disease Using Mass Spectrometry

      • Nagihan Bostanci, Kai Bao
      Pages 13-23

    4. Saliva Diagnosis Using Small Extracellular Vesicles and Salivaomics

      • Pingping Han, Xiang Li, Wei Wei, Sašo Ivanovski
      Pages 25-39

    5. Antioxidant Micronutrients and Oxidative Stress Biomarkers

      • Irundika H. K. Dias, Helen R. Griffiths, Mike R. Milward, Martin R. Ling, Iain L. C. Chapple, Melissa M. Grant
      Pages 41-58

  3. Molecular Biosciences

    1. Front Matter

      Pages 59-59
      Download chapter PDF

    2. The Oral Microbiota in Health and Disease: An Overview of Molecular Findings

      • José F. Siqueira Jr., Isabela N. Rôças
      Pages 61-73

    3. The Long and Short of Genome Sequencing: Using a Hybrid Sequencing Strategy to Sequence Oral Microbial Genomes

      • Nicholas C. K. Heng, Jo-Ann L. Stanton
      Pages 75-89

    4. Microbial Community Profiling Using Terminal Restriction Fragment Length Polymorphism (T-RFLP) and Denaturing Gradient Gel Electrophoresis (DGGE)

      • José F. Siqueira Jr, Mitsuo Sakamoto, Alexandre S. Rosado
      Pages 91-104

    5. Bioinformatic Approaches for Describing the Oral Microbiota

      • Kristi Biswas, Michael W. Taylor, David T. J. Broderick
      Pages 105-130

    6. Adhesion of Yeast and Bacteria to Oral Surfaces

      • Richard D. Cannon, Karl M. Lyons, Kenneth Chong, Kathryn Newsham-West, Kyoko Niimi, Ann R. Holmes
      Pages 131-156

    7. Quantitative Analysis of Periodontal Pathogens Using Real-Time Polymerase Chain Reaction (PCR)

      • Mª. José Marin, Elena Figuero, David Herrera, Mariano Sanz
      Pages 157-169

    8. Methods to Study Antagonistic Activities Among Oral Bacteria

      • Fengxia Qi, Jens Kreth
      Pages 171-186

    9. Generation of Multispecies Oral Bacteria Biofilm Models

      • Jason L. Brown, Mark C. Butcher, Chandra Lekha Ramalingam Veena, Safa Chogule, William Johnston, Gordon Ramage
      Pages 187-199

    10. Markerless Genome Editing in Competent Streptococci

      • Roger Junges, Rabia Khan, Yanina Tovpeko, Heidi A. Åmdal, Fernanda C. Petersen, Donald A. Morrison
      Pages 201-216

    11. A Protocol to Produce Genetically Edited Primary Oral Keratinocytes Using the CRISPR-Cas9 System

      • Sven E. Niklander, Keith D. Hunter
      Pages 217-229

    12. Size-Based Method for Enrichment of Circulating Tumor Cells from Blood of Colorectal Cancer Patients

      • Sai Shyam Vasantharajan, Edward Barnett, Elin S. Gray, Euan J. Rodger, Michael R. Eccles, Sharon Pattison et al.
      Pages 231-248

    13. Strategy for RNA-Seq Experimental Design and Data Analysis

      • Gregory Gimenez, Peter A. Stockwell, Euan J. Rodger, Aniruddha Chatterjee
      Pages 249-278

    14. Characterization of the Expression and Role of Histone Acetylation and Deacetylation in Dental Pulp Cells

      • Yukako Yamauchi, Henry F. Duncan
      Pages 279-293
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Keywords

About this book

This fully revised new edition explores advances in the prevention and treatment of oral diseases. Beyond the updated chapters, the book delves into regenerative biology, gene editing and the use of CRISPR in oral biology, as well as histone acetylation and deacetylation methods, further reflecting advances in the application of molecular techniques to oral biology. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and up-to-date, Oral Biology: Molecular Techniques and Applications, Third Edition serves as an ideal basic resource not only for new researchers but also for experienced scientists wishing to expand their research platform into new areas of this vital field.

Editors and Affiliations

  • School of Dentistry, The University of Queensland, Herston, Australia

    Gregory J. Seymour, Mary P. Cullinan

  • Faculty of Dentistry, University of Otago, Dunedin, New Zealand

    Nicholas C.K. Heng, Paul R. Cooper

Bibliographic Information

  • Book Title: Oral Biology

  • Book Subtitle: Molecular Techniques and Applications

  • Editors: Gregory J. Seymour, Mary P. Cullinan, Nicholas C.K. Heng, Paul R. Cooper

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-2780-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2023

  • Hardcover ISBN: 978-1-0716-2779-2Published: 24 November 2022

  • Softcover ISBN: 978-1-0716-2782-2Published: 24 November 2023

  • eBook ISBN: 978-1-0716-2780-8Published: 23 November 2022

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 3

  • Number of Pages: XV, 528

  • Number of Illustrations: 31 b/w illustrations, 91 illustrations in colour

  • Topics: Anatomy, Biomedicine, general

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