Skip to main content
SpringerLink
Log in

CCN Proteins

Methods and Protocols

  • Book
  • © 2023
  • Latest edition

Overview

Editors:
  1. Masaharu Takigawa

    1. Advanced Research Center for Oral and Craniofacial Sciences, Okayama University Dental School/Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2582)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 249.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (28 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. CCN Proteins (Cellular Communication Network Factors): Expanding Their Repertoire Toward a New Concept

    • Masaharu Takigawa
    Pages 1-10

  3. Basic Methods and Protocols

    1. Front Matter

      Pages 11-11
      Download chapter PDF

    2. Optimal CCN4 Immunofluorescence for Tissue Microarray

      • Pierre-Olivier Gaudreau, Isabelle Cousineau, John Stagg
      Pages 13-21

    3. Cellular Fluorescence Imaging for the Evaluation of Bioactivity of CCN Family Proteins

      • Harumi Kawaki, Satoshi Kubota, Masaharu Takigawa
      Pages 23-29

    4. Imaging of Molecular Interaction Between CCN Protein and Its Binding Partners: An In Situ Proximity Ligation Assay of Interaction Between CCN2 and Rab14 in Chondrocytes

      • Mitsuhiro Hoshijima, Eriko Aoyama, Hiroshi Kamioka, Masaharu Takigawa
      Pages 31-37

    5. Western Blot Protocols for Analysis of CCN Proteins and Fragments in Exosomes, Vesicle-Free Fractions, and Cells

      • Kisho Ono, Yuka Okusha, Manh Tien Tran, Koki Umemori, Takanori Eguchi
      Pages 39-57

    6. Comprehensive Method for Exosome Isolation and Proteome Analysis for Detection of CCN Factors in/on Exosomes

      • Takanori Eguchi, Yuka Okusha, Yanyin Lu, Kisho Ono, Eman A. Taha, Shiro Fukuoka
      Pages 59-76

    7. Evaluation of the Molecular Interaction Between CCN Protein and Its Binding Partners: A Solid-Phase Binding Assay and Surface Plasmon Resonance

      • Eriko Aoyama, Masaharu Takigawa
      Pages 77-86

    8. Protocols for Screening Peptides Binding to CCN Family Proteins and Their Extended Utility

      • Satoshi Kubota, Harumi Kawaki, Masaharu Takigawa
      Pages 87-101

    9. Transfection, Spinfection, Exofection, and Luciferase Assays for Analysis of CCN Genes Expression Mechanism

      • Takanori Eguchi, Yanyin Lu, Eman A. Taha, Yuka Okusha
      Pages 103-126

    10. Analyses of the Posttranscriptional Regulation of CCN Genes: Approach to Multiple Steps of CCN2 Gene Expression

      • Seiji Kondo, Satoshi Kubota, Masaharu Takigawa
      Pages 127-155

    11. Protocol for CRISPR/Cas Genome Editing for Investigating Cell Communication Network

      • Yuka Okusha, Takanori Eguchi
      Pages 157-167

    12. Utilizing Public Molecular Biological Databases for CCN Family Research

      • Satoshi Kubota
      Pages 169-187

  4. Functional Analysis

    1. Front Matter

      Pages 189-189
      Download chapter PDF

    2. Evaluation of the Effects of CCN4 on Pancreatic Beta Cell Proliferation

      • Rebeca Fernández-Ruiz, Rosa Gasa
      Pages 191-208

    3. Analysis of CCN Functions in Liver Regeneration After Partial Hepatectomy

      • Naiyuan Cheng, Ki-Hyun Kim, Lester F. Lau
      Pages 209-221

    4. The Evaluation of Meniscus Regenerative Effects of LIPUS-Induced CCN Proteins: Induction by LIPUS of CCN2 and Meniscus-Related Genes in Cultured Meniscus Cells and Meniscus Tissues

      • Yusuke Kamatsuki, Eriko Aoyama, Takayuki Furumatsu, Toshifumi Ozaki, Masaharu Takigawa
      Pages 223-235

    5. Analysis of Chemotactic Property of CCN2/CTGF in Intramembranous Osteogenesis

      • Nobuo Takeshita, Teruko Takano-Yamamoto
      Pages 237-253

    6. Novel Cell Biological Assays for Measuring Bone Remodeling Activities of CCN Proteins

      • Takashi Nishida, Satoshi Kubota, Masaharu Takigawa
      Pages 255-268
Back to top

Keywords

About this book

This second edition volume expands on the previous edition with updated methodologies and practical tips to overcome obstacles associated with experimentation pertaining to chemistry, biology, physiology, pathology, medical and dental sciences, and pharmacology of CCN proteins. The chapters in this book cover topics such as CCN4 immunofluorescence for tissue microarray; utilizing public molecular biological databases for CCN family research; the effects of CCN4 on pancreatic beta cell proliferation; gene expression analysis of CCNs; novel cell biological assays for measuring bone remodeling activities of CCN proteins; and the function of CCN2 in tubular epithelium cells with a focus on renal fibrogenesis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, CCN Proteins: Methods and Protocols, Second Edition is a valuable resource for experienced CCN researchers looking for new approaches and novice researchers just starting out in the field of CCN research.

Editors and Affiliations

  • Advanced Research Center for Oral and Craniofacial Sciences, Okayama University Dental School/Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan

    Masaharu Takigawa

Bibliographic Information

  • Book Title: CCN Proteins

  • Book Subtitle: Methods and Protocols

  • Editors: Masaharu Takigawa

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-2744-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2023

  • Hardcover ISBN: 978-1-0716-2743-3Published: 13 November 2022

  • Softcover ISBN: 978-1-0716-2746-4Published: 14 November 2023

  • eBook ISBN: 978-1-0716-2744-0Published: 12 November 2022

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XIV, 430

  • Number of Illustrations: 20 b/w illustrations, 66 illustrations in colour

  • Topics: Chemistry/Food Science, general, Cell Biology

Publish with us

Policies and ethics

Back to top

Search

Navigation