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T-Cell Development

Methods and Protocols

  • Book
  • © 2023
  • Latest edition

Overview

Editors:
  1. Remy Bosselut

    1. Laboratory of Immune Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. Melanie S. Vacchio

    1. Laboratory of Immune Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2580)

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Table of contents (22 protocols)

  1. Front Matter

    Pages i-xi
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  2. Background Information

    1. Front Matter

      Pages 1-1
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    2. A Beginner’s Guide to T Cell Development

      • Rémy Bosselut
      Pages 3-24

    3. Revelations in Thymic Epithelial Cell Biology and Heterogeneity from Single-Cell RNA Sequencing and Lineage Tracing Methodologies

      • Abigail Morales-Sanchez, Susannah C. Shissler, Jennifer E. Cowan, Avinash Bhandoola
      Pages 25-49

    4. Early Development of Innate Lymphoid Cells

      • Yi Ding, Christelle Harly, Arundhoti Das, Avinash Bhandoola
      Pages 51-69

    5. Development of γδ T Cells: Soldiers on the Front Lines of Immune Battles

      • Alejandra V. Contreras, David L. Wiest
      Pages 71-88

    6. Principles of Advanced Flow Cytometry: A Practical Guide

      • Shafiuddin Siddiqui, Ferenc Livák
      Pages 89-114

  3. Core Approaches and Strategies

    1. Front Matter

      Pages 115-115
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    2. Genetic Strategies to Study T Cell Development

      • Rémy Bosselut
      Pages 117-130

    3. A Simplified Approach to Evaluating T Cell Development by Flow Cytometry

      • Jan Y. M. Lee, Paul E. Love
      Pages 131-149

    4. Purification of Thymocyte and T Cell Subsets

      • Andrea C. Carpenter, Laura B. Chopp, Jong Kyong Kim, Rémy Bosselut
      Pages 151-163

    5. Generation of Bone Marrow Chimeras

      • Yayi Gao, Rémy Bosselut
      Pages 165-171

    6. Generation of Murine T Cell Effector Populations In Vitro

      • Melanie S. Vacchio
      Pages 173-186

  4. Specific Techniques

    1. Front Matter

      Pages 187-187
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    2. Large-Scale Isolation of Mouse Thymic Epithelial Cells

      • Izumi Ohigashi, Mami Matsuda-Lennikov, Yousuke Takahama
      Pages 189-197

    3. Generation of Retrogenic Mice to Investigate T Cell Development

      • Yayi Gao, Rémy Bosselut
      Pages 199-209

    4. Purification of Bone Marrow Precursors to T Cells and ILCs

      • Devin Kenney, Christelle Harly
      Pages 211-232

    5. Studying T Cell Development in Neonatal and Adult Thymic Slices

      • Laura L. McIntyre, Lydia K. Lutes, Ellen A. Robey
      Pages 233-247

    6. Induction of Human T Cell Development In Vitro with OP9-DL4-7FS Cells Expressing Human Cytokines

      • Mahmood Mohtashami, Patrick M. Brauer, Juan Carlos Zúñiga-Pflücker
      Pages 249-260

    7. Methods for Study of Mouse T Cell Receptor α and β Gene Rearrangements

      • Danielle J. Dauphars, Glendon Wu, Craig H. Bassing, Michael S. Krangel
      Pages 261-282
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Keywords

About this book

This second edition volume provides new and updated chapters detailing simple and accessible experiment protocols to explore thymus biology. Chapters are divided into three parts presenting short reviews, analysis strategies, protocols for cell preparation, flow cytometry analyses, Innate Lymphoid Cells (ILC), mouse T-cell development, antigen receptor-less cousins of T cells, bone marrow chimeras, thymic stroma, and multiple aspects of thymocyte biology. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, T-Cell Development: Methods and Protocols, Second Edition aims to be a useful practical guide to help readers overcome obstacles associated with experimental approaches of T-cell development.

Editors and Affiliations

  • Laboratory of Immune Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, USA

    Remy Bosselut, Melanie S. Vacchio

Bibliographic Information

  • Book Title: T-Cell Development

  • Book Subtitle: Methods and Protocols

  • Editors: Remy Bosselut, Melanie S. Vacchio

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-2740-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply 2023

  • Hardcover ISBN: 978-1-0716-2739-6Published: 15 November 2022

  • Softcover ISBN: 978-1-0716-2742-6Published: 15 November 2023

  • eBook ISBN: 978-1-0716-2740-2Published: 14 November 2022

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XI, 384

  • Number of Illustrations: 35 b/w illustrations, 41 illustrations in colour

  • Topics: Immunology, Cancer Research

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