Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 2580)
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Table of contents (22 protocols)
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Front Matter
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Background Information
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Front Matter
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Core Approaches and Strategies
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Front Matter
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Specific Techniques
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Front Matter
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About this book
This second edition volume provides new and updated chapters detailing simple and accessible experiment protocols to explore thymus biology. Chapters are divided into three parts presenting short reviews, analysis strategies, protocols for cell preparation, flow cytometry analyses, Innate Lymphoid Cells (ILC), mouse T-cell development, antigen receptor-less cousins of T cells, bone marrow chimeras, thymic stroma, and multiple aspects of thymocyte biology. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, T-Cell Development: Methods and Protocols, Second Edition aims to be a useful practical guide to help readers overcome obstacles associated with experimental approaches of T-cell development.
Editors and Affiliations
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Laboratory of Immune Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, USA
Remy Bosselut, Melanie S. Vacchio
Bibliographic Information
Book Title: T-Cell Development
Book Subtitle: Methods and Protocols
Editors: Remy Bosselut, Melanie S. Vacchio
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-0716-2740-2
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply 2023
Hardcover ISBN: 978-1-0716-2739-6Published: 15 November 2022
Softcover ISBN: 978-1-0716-2742-6Published: 15 November 2023
eBook ISBN: 978-1-0716-2740-2Published: 14 November 2022
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 2
Number of Pages: XI, 384
Number of Illustrations: 35 b/w illustrations, 41 illustrations in colour
Topics: Immunology, Cancer Research