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  • © 2023

Epigenomics

Methods and Protocols

Editors:

  1. Izuho Hatada

    1. Institute for Molecular and Cellular Regulation, Gunma University, Maebashi, Japan

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Takuro Horii

    1. Institute for Molecular and Cellular Regulation, Gunma University, Maebashi, Japan

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2577)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xi
    PDF

  2. Analysis of the Epigenome: DNA Methylation

    1. Front Matter

      Pages 1-1
      PDF

    2. DNA Methylation Analysis Using Bisulfite Pyrosequencing

      • Ken Higashimoto, Satoshi Hara, Hidenobu Soejima
      Pages 3-20

    3. Post-bisulfite Adaptor Tagging Based on an ssDNA Ligation Technique (tPBAT)

      • Fumihito Miura, Yukiko Shibata, Miki Miura, Takashi Ito
      Pages 21-37

    4. Reduced Representation Bisulfite Sequencing (RRBS)

      • Kazuhiko Nakabayashi, Michihiro Yamamura, Keita Haseagawa, Kenichiro Hata
      Pages 39-51

  3. Analysis of the Epigenome: Histone Modification and Chromatin Structure

    1. Front Matter

      Pages 53-53
      PDF

    3. ATAC-Seq Analysis of Accessible Chromatin: From Experimental Steps to Data Analysis

      • Mayu Tatara, Taiyo Ikeda, Satoshi H. Namekawa, So Maezawa
      Pages 65-81

    7. Bioinformatics Pipelines for Identification of Super-Enhancers and 3D Chromatin Contacts

      • Akihiko Sakashita, Chikara Takeuchi, So Maezawa, Satoshi H. Namekawa
      Pages 123-146

    9. Solubilization of Mouse Sperm Chromatin for Sequencing Analyses Using a Chaperon Protein

      • Yuko Fukuda, Keishi Shintomi, Kosuke Yamaguchi, Yasuhiro Fujiwara, Yuki Okada
      Pages 161-173

  4. Manipulation of the Epigenome: Platforms for Epigenome Editing

    1. Front Matter

      Pages 175-175
      PDF

    2. Efficient Targeted DNA Methylation with dCas9-Coupled DNMT3A-DNMT3L Methyltransferase

      • Pavel Bashtrykov, Nivethika Rajaram, Albert Jeltsch
      Pages 177-188

    3. Regulation of Gene Expression Using dCas9-SunTag Platforms

      • Sumiyo Morita, Takuro Horii, Izuho Hatada
      Pages 189-195

    5. Design, Construction, and Validation of Targeted Gene Activation with TREE System in Human Cells

      • Atsushi Kunii, Takashi Yamamoto, Tetsushi Sakuma
      Pages 211-226

  5. Manipulation of the Epigenome: Inducible System for Epigenome Editing

    1. Front Matter

      Pages 227-227
      PDF
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About this book

This detailed volume provides a comprehensive collection of protocols for epigenomic research, powering our ability to analyze epigenetic modifications across the entire genome. Beginning with methods used to investigate epigenomic modifications such as DNA methylation, histone modifications, and chromatin structure, the book continues with methods for manipulating the epigenome, including platforms for epigenome editing, inducible systems for epigenome editing, and epigenetically modified animals. Written for the highly successful Methods in Molecular Biology series, chapters feature introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Epigenomics: Methods and Protocols serves as an ideal resource for researchers looking to further expand the utility and scope of epigenomics research.

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Keywords

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Editors and Affiliations

  • Institute for Molecular and Cellular Regulation, Gunma University, Maebashi, Japan

    Izuho Hatada, Takuro Horii

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Bibliographic Information

  • Book Title: Epigenomics

  • Book Subtitle: Methods and Protocols

  • Editors: Izuho Hatada, Takuro Horii

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-2724-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2023

  • Hardcover ISBN: 978-1-0716-2723-5Published: 30 September 2022

  • Softcover ISBN: 978-1-0716-2726-6Published: 02 October 2023

  • eBook ISBN: 978-1-0716-2724-2Published: 29 September 2022

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 296

  • Number of Illustrations: 11 b/w illustrations, 64 illustrations in colour

  • Topics: Genetics and Genomics, Biological Techniques

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Access via your institution

Buy it now

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

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