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Gene, Drug, and Tissue Engineering

  • Book
  • © 2023

Overview

Editors:
  1. Glaucia C. Pereira

    1. Bioengineering Department, Imperial College London, London, UK

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2575)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-xii
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  2. Molecular Genetics and Genomics

    1. Front Matter

      Pages 1-1
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    2. Gene Alterations Induced by Glutamine (Q) Encoding CAG Repeats Associated with Neurodegeneration

      • Gundappa Saha, Sukanya Ghosh, Vikash Kumar Dubey, Prakash Saudagar
      Pages 3-23

    3. Latest Trends in Nucleic Acids’ Engineering Techniques Applied to Precision Medicine

      • Glaucia C. Pereira
      Pages 25-38

    4. Nanotechnology-Driven Delivery Systems in Inoculation Therapies

      • Glaucia C. Pereira
      Pages 39-57

  3. Molecular Biology in Drug Development and Therapeutics

    1. Front Matter

      Pages 59-59
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    2. Chronic Kidney Disease: Challenges in Translational Medicine

      • Bruno Freire Botelho, André Luis Barreira, Maurilo Leite Jr, Marcelo Marcos Morales
      Pages 61-75

    3. Mass Spectroscopy as an Analytical Tool to Harness the Production of Secondary Plant Metabolites: The Way Forward for Drug Discovery

      • Faheem Ahmad, Hera Nadeem
      Pages 77-103

    4. Assessing the Challenges of Nanotechnology-Driven Targeted Therapies: Development of Magnetically Directed Vectors for Targeted Cancer Therapies and Beyond

      • Matthew T. Bryan
      Pages 105-123

  4. Molecular, Cellular, Tissue Engineering, and AI-Based Computational Design

    1. Front Matter

      Pages 125-125
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    2. Advances in Regenerative Medicine and Biomaterials

      • Şükran Åžeker, AyÅŸe Eser Elçin, YaÅŸar Murat Elçin
      Pages 127-152

    3. A Brief Review of Plant Cell Transfection, Gene Transcript Expression, and Genotypic Integration for Enhancing Compound Production

      • Munish Sharma, Sakshi Bhushan, Deepak Sharma, Sanjana Kaul, Manoj K. Dhar
      Pages 153-179

    4. A Review of Stem Cell Technology Targeting Hepatocyte Growth as an Alternative to Organ Transplantation

      • Ernesto Goulart
      Pages 181-193

    5. Novel Nanotechnology-Driven Prototypes for AI-Enriched Implanted Prosthetics Following Organ Failure

      • Glaucia C. Pereira
      Pages 195-237

  5. Methods and Protocols

    1. Front Matter

      Pages 239-239
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    2. Simplified CRISPR-Mediated DNA Editing in Multicellular Eukaryotes

      • Ritesh Kumar, Kartikeya Tiwari, Prakash Saudagar
      Pages 241-260

    3. Design of SaCas9-HF for In Vivo Gene Therapy

      • Kartikeya Tiwari, Ritesh Kumar, Prakash Saudagar
      Pages 261-268

    4. Stem Cell Technology in Organ Transplantation: A Novel Method for 3D Bioprinting Functional and Stable Liver Grafts Using Human iPS Cells Derived Cells

      • Ernesto Goulart
      Pages 269-274

    5. In Vitro Assay for the Assessment of Oxygen Depletion Triggers in Human Cell Lines, Associated with Improving Responses to Cancer Therapy

      • Kartikeya Tiwari, Ritesh Kumar, Gundappa Saha, Prakash Saudagar
      Pages 275-295

    6. The Use of 99mTc-Labeled Stem Cells for Evaluation of Chronic Kidney Disease

      • Bianca Gutfilen, Sergio Augusto Lopes de Souza, Marcelo Marcos Morales
      Pages 297-304
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Keywords

About this book

This book combines discursive chapters that present the latest progress in molecular biology, drug discovery, organ-tissue engineering, and related fields, with a number of descriptive chapters on methods, protocols, and case studies. Structured into four parts, this volume walks the reader through the latest in cellular biology, with discussions on novel medicinal plant metabolites, nanotechnology in precision medicine, nucleic acid-based therapeutics and vaccines, genetic engineering, computational aid, bioinformatics, synthetic organs for transplantation, and organ-tissue engineering. Written for the highly successful Methods in Molecular Biology series, chapters include the kind of detail and expert implementation advice that ensures quality results in the lab. 

Authoritative and informative, Gene, Drug, and Tissue Engineering serves as an ideal guide for undergraduate students, postgraduate researchers, and senior researchers working in biomedicine and its underlying technologies, stimulating both computational and experimental development and fostering the exchange of new ideas.

Editors and Affiliations

  • Bioengineering Department, Imperial College London, London, UK

    Glaucia C. Pereira

Bibliographic Information

  • Book Title: Gene, Drug, and Tissue Engineering

  • Editors: Glaucia C. Pereira

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-2716-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2023

  • Hardcover ISBN: 978-1-0716-2715-0Published: 28 October 2022

  • Softcover ISBN: 978-1-0716-2718-1Published: 28 October 2023

  • eBook ISBN: 978-1-0716-2716-7Published: 27 October 2022

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 345

  • Number of Illustrations: 1 b/w illustrations

  • Topics: Biological Techniques, Regenerative Medicine/Tissue Engineering, Pharmaceutical Sciences/Technology

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