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Tumor Angiogenesis Assays

Methods and Protocols

  • Book
  • © 2023

Overview

Editors:
  1. Domenico Ribatti

    1. Department of Basic Medical Sciences, Neurosciences and Sensory Organs, Section of Human Anatomy and Histology, University of Bari Medical School, Bari, Italy

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2572)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Discovery and Development of Tumor Angiogenesis Assays

    • Gianfranco Natale, Guido Bocci
    Pages 1-37

  3. The Role of the Stroma in Tumor Angiogenesis and Progression: The Fundamental Contribution of Pietro M. Gullino

    • Domenico Ribatti
    Pages 39-44

  4. Methods for Isolation of Tumor-Associated Endothelial Cells for Surface Protein Analysis and Sorting by Flowcytometry

    • Jelle Verhoeven, Patrizia Agostinis, Madhur Agrawal
    Pages 45-54

  5. Perfusion-Based Fluorescent Dye Labeling to Sort Cancer Cells Based on Their Distance from Blood Vessels

    • Saran Kumar
    Pages 55-66

  6. Visualizing the Interactions Shaping the Imaging of the Microenvironment in Human Cancers

    • Antonio G. Solimando, Vanessa Desantis, Matteo Claudio Da Vià
    Pages 67-79

  7. Quantification of Tumor and Angiogenesis-Related Markers in Ovarian Cancer Models by a Digital Pathology Approach

    • Ilaria Piga, Sonia Minuzzo, Tiziana Zanin, Giovanni Esposito, Stefano Indraccolo
    Pages 81-89

  8. Monitoring Angiotropic Extravascular Migratory Metastasis In Vitro

    • Claire Lugassy, Hynda K. Kleinman, Raymond L. Barnhill
    Pages 91-100

  9. Double Immunohistochemical Staining on Formalin-Fixed Paraffin-Embedded Tissue Samples to Study Vascular Co-option

    • Tiziana Annese, Mariella Errede, Michelina De Giorgis, Loredana Lorusso, Roberto Tamma, Domenico Ribatti
    Pages 101-116

  10. Development of Experimental Three-Dimensional Tumor Models to Study Glioblastoma Cancer Stem Cells and Tumor Microenvironment

    • Henry Ruiz-Garcia, Natanael Zarco, Fumihiro Watanabe, Virginea De Araujo Farias, Paola Suarez-Meade, Hugo Guerrero-Cazares et al.
    Pages 117-127

  11. IKOSA® CAM Assay Application to Quantify Blood Vessels on Chick Chorioallantoic Membrane (CAM)

    • Tiziana Annese, Roberto Tamma, Domenico Ribatti
    Pages 129-139

  12. Application of Laser Speckle Contrast Imaging (LSCI) for the Angiogenesis Measurement of Tumors in the Chorioallantoic Membrane (CAM) Model

    • Eric Pion, Silke Haerteis, Thiha Aung
    Pages 141-153

  13. The Chick CAM as an In Vivo System to Study Stem Cell Activity

    • Marta Teixeira Pinto, Ana Sofia Ribeiro, Joana Paredes
    Pages 155-166

  14. Detection of Possible Symmetries in Vascular Networks by Computer-Assisted Image Analysis

    • Diego Guidolin, Cinzia Tortorella, Domenico Ribatti
    Pages 167-180

  15. Alternative In Vivo Models to Study Teratoma

    • Michela Corsini, Stefania Mitola
    Pages 181-189

  16. How to Generate a Vascular-Labelled Transgenic Zebrafish Model to Study Tumor Angiogenesis and Extravasation

    • Roxana E. Oberkersch, Jacopo Lidonnici, Massimo M. Santoro
    Pages 191-202

  17. 3D Human Tumor Tissues Cultured in Dynamic Conditions as Alternative In Vitro Disease Models

    • Giorgia Pelizzoni, Silvia Scaglione
    Pages 203-210

  18. Back Matter

    Pages 211-212
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Keywords

About this book

This second edition provides new and updated methods and protocols for studying tumor angiogenesis in vitro and in vivo. Chapters detail morphological aspects of tumor angiogenesis, aortic ring, ex vivo tissue culture model for anti-angiogenic drug testing, transgenic zebrafish, orthotopic models of ovarian cancer, and uncovering metabolic effects of anti-angiogenic therapy in tumors by induced metabolic bioluminescence imaging.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Tumor Angiogenesis Assays: Methods and Protocols, Second Edition is a valuable resource for all researchers interested in learning more about this important and developing field.

Editors and Affiliations

  • Department of Basic Medical Sciences, Neurosciences and Sensory Organs, Section of Human Anatomy and Histology, University of Bari Medical School, Bari, Italy

    Domenico Ribatti

Bibliographic Information

  • Book Title: Tumor Angiogenesis Assays

  • Book Subtitle: Methods and Protocols

  • Editors: Domenico Ribatti

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-2703-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2023

  • Hardcover ISBN: 978-1-0716-2702-0Published: 27 September 2022

  • Softcover ISBN: 978-1-0716-2705-1Published: 28 September 2023

  • eBook ISBN: 978-1-0716-2703-7Published: 26 September 2022

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XI, 212

  • Number of Illustrations: 6 b/w illustrations, 49 illustrations in colour

  • Topics: Cancer Research

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