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Nucleic Acid Aptamers

Selection, Characterization, and Application

  • Book
  • © 2023

Overview

Editors:
  1. Günter Mayer

    1. Life and Medical Sciences Institute (LIMES), University of Bonn, Bonn, Germany

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    You can also search for this editor in PubMed   Google Scholar

  2. Marcus M. Menger

    1. Branch Bioanalytics and Bioprocesses, Fraunhofer Institute for Cell Therapy and Immunology, Potsdam, Germany

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2570)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xi
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  2. Selection

    1. Front Matter

      Pages 1-1
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    2. Automated ssDNA SELEX Using Histidine-Tagged Target Proteins

      • Stefan Breuers, Günter Mayer
      Pages 3-11

    3. Discovery of Aptamers Against Cell Surface Markers Using Ligand-Guided Selection

      • Nicole Williams, Rutika Patel, Prabodhika Mallikaratchy
      Pages 13-38

    4. Implementation of Emulsion PCR for Amplification of Click-Modified DNA During SELEX

      • Moujab Choukeife, Anna Jonczyk, Günter Mayer
      Pages 39-44

    5. Preparation of Chemically Modified DNA Library for SELEX via Incorporation of CLB-dUTP in Primer Extension Reaction

      • Magda Puchała, Marta Radzińska, Joanna Guzdek, Agnieszka Sok-Grochowska, Aleksandra Adamowicz-Skrzypkowska, Paweł Pięta et al.
      Pages 45-61

    6. RNA Capture-SELEX on Streptavidin Magnetic Beads

      • Leon Kraus, Beatrix Suess
      Pages 63-71

    7. The Bioinformatics of Aptamers: HT-SELEX Analysis with AptaSUITE

      • Jan Hoinka, Teresa M. Przytycka
      Pages 73-83

    8. Improvement of Aptamers by High-Throughput Sequencing of Doped-SELEX

      • Frédéric Ducongé
      Pages 85-102

  3. Characterization

    1. Front Matter

      Pages 103-103
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    2. Analysis of Aptamer-Small Molecule Binding Interactions Using Isothermal Titration Calorimetry

      • Sladjana Slavkovic, Philip E. Johnson
      Pages 105-118

    3. Determination of Aptamer Structure Using Circular Dichroism Spectroscopy

      • Yannick Kerler, Stephan Sass, Carsten Hille, Marcus M. Menger
      Pages 119-128

    4. Immobilization-Free Determination of Dissociation Constants Independent of Ligand Size Using MicroScale Thermophoresis

      • Wiebke Sabrowski, Walter F. M. Stöcklein, Marcus M. Menger
      Pages 129-140

    5. Label-Free Determination of the Kinetic Parameters of Protein-Aptamer Interaction by Surface Plasmon Resonance

      • Nico Dreymann, Anja Möller, Marcus M. Menger
      Pages 141-153

    6. Probing of Fluorogenic RNA Aptamers via Supramolecular Förster Resonance Energy Transfer with a Universal Fluorescent Nucleobase Analog

      • Christian Steinmetzger, Claudia Höbartner
      Pages 155-173

  4. Application

    1. Front Matter

      Pages 175-175
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    2. Slow-Off-Rate-Modified Aptamer Labeling for Fluorescence Microscopy and DNA-PAINT

      • Sebastian Strauss, Ralf Jungmann
      Pages 177-185

    3. Aptamer–Field-Effect Transistors for Small-Molecule Sensing in Complex Environments

      • Nako Nakatsuka
      Pages 187-196

    4. Aptasensor for Impedimetric Detection of Lysozyme

      • Ece Eksin, Huseyin Senturk, Arzum Erdem
      Pages 197-204

    5. Biophysical Analysis of Small Molecule Binding to Viral RNA Structures

      • Liang-Yuan Chiu, Jesse Davila-Calderon, Zhengguo Cai, Blanton S. Tolbert
      Pages 205-222
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Keywords

About this book

This updated book reflects improvements in a variety of techniques used to study the aptamer field. Beginning with a section on selection procedures, the volume continues with methods to characterize aptamers’ interaction and structural properties by biophysical approaches, as well as a variety of applications that have been adapted to the aptamer compound class. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and up-to-date, Nucleic Acid Aptamers: Selection, Characterization, and Application, Second Edition serves as an ideal guide for researchers aiming to further our understanding of aptamer biology and more.

Editors and Affiliations

  • Life and Medical Sciences Institute (LIMES), University of Bonn, Bonn, Germany

    Günter Mayer

  • Branch Bioanalytics and Bioprocesses, Fraunhofer Institute for Cell Therapy and Immunology, Potsdam, Germany

    Marcus M. Menger

Bibliographic Information

  • Book Title: Nucleic Acid Aptamers

  • Book Subtitle: Selection, Characterization, and Application

  • Editors: Günter Mayer, Marcus M. Menger

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-2695-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2023

  • Hardcover ISBN: 978-1-0716-2694-8Published: 27 September 2022

  • Softcover ISBN: 978-1-0716-2697-9Published: 28 September 2023

  • eBook ISBN: 978-1-0716-2695-5Published: 26 September 2022

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XI, 282

  • Number of Illustrations: 12 b/w illustrations, 66 illustrations in colour

  • Topics: Chemistry/Food Science, general, Biomaterials, Biological Techniques

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