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Hematopoietic Stem Cells

Methods and Protocols

  • Book
  • © 2023

Overview

Editors:
  1. Louis M. Pelus

    1. Department of Microbiology & Immunology and Department of Medicine/Hematology Oncology, Indiana University School of Medicine, Indianapolis, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. Jonathan Hoggatt

    1. Moderna Therapeutics, Cambridge, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2567)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Overview

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. The Factory of Blood Production: Hematopoietic Stem Cells

      • Jonathan Hoggatt, Louis M. Pelus
      Pages 3-7

  3. Molecular Modification and Evaluation

    1. Front Matter

      Pages 9-9
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    2. Detection of DNA Damage in Hematopoietic Stem Cells

      • Saipriya Ayyar, Isabel Beerman
      Pages 11-28

    3. Evaluating Histone Acetylation in Mouse Hematopoietic Stem and Progenitor Cells Using Chromatin Immunoprecipitation

      • Liqiong Liu
      Pages 29-38

    4. CRISPR Gene Editing of Hematopoietic Stem and Progenitor Cells

      • Reza Shahbazi, Patricia Lipson, Karthikeya S. V. Gottimukkala, Daniel D. Lane, Jennifer E. Adair
      Pages 39-62

    5. Lentiviral Transduction of Nonhuman Primate Hematopoietic Stem and Progenitor Cells

      • Chuanfeng Wu, So Gun Hong, Aylin Bonifacino, Cynthia E. Dunbar
      Pages 63-84

  4. Cellular Isolation and Characterization

    1. Front Matter

      Pages 85-85
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    2. Identification of Nonhuman Primate Hematopoietic Stem and Progenitor Cells

      • Stefan Radtke, Hans-Peter Kiem
      Pages 87-98

    3. Isolation and Characterization of Fetal Liver Hematopoietic Stem Cells

      • Diego A. López, Anna E. Beaudin
      Pages 99-112

    4. Utilizing CyTOF to Examine Hematopoietic Stem and Progenitor Phenotype

      • Safa F. Mohamad, Maegan L. Capitano
      Pages 113-126

    5. Hematopoietic Stem Cell Identification Postirradiation

      • Andrea M. Patterson, Christie M. Orschell, Louis M. Pelus
      Pages 127-140

  5. The Hematopoietic Niche and Accessory Cells

    1. Front Matter

      Pages 141-141
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    2. Intravital Microscopy for Hematopoietic Studies

      • Myriam L. R. Haltalli, Cristina Lo Celso
      Pages 143-162

    3. Laser Micromachining of Bone as a Tool for Studying Bone Marrow Biology

      • Christa Haase, Dmitry Richter, Charles P. Lin
      Pages 163-180

    4. MSC and HSPC Coculture: Mimicking Ex Vivo Bone Marrow Niche

      • Pratibha Singh
      Pages 181-189

    5. Isolation of Thymus Stromal Cells from Human and Murine Tissue

      • Karin Gustafsson, David T. Scadden
      Pages 191-201

  6. Transplantation and In Vivo Models

    1. Front Matter

      Pages 203-203
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    2. Experimental Models of Mouse and Human Hematopoietic Stem Cell Transplantation

      • Scott H. Cooper, Maegan L. Capitano, Hal E. Broxmeyer
      Pages 205-232
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Keywords

About this book

This detailed volume collects updates on the technical advances in hematopoietic stem cell research and incorporates new techniques focused on the molecular/genetic, cellular, and whole organism levels. Exploring methods that apply stress to hematopoiesis, the book also contains chapters focused on better understanding the role of hematopoietic niches and their cellular components, as well as in vivo models that test and quantitate stem cell function and are key to further development of therapeutic applications. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and up-to-date, Hematopoietic Stem Cells: Methods and Protocols serves as a valued addition to laboratories focused on understanding hematopoietic stem cell biology and the therapeutic advances that can be derived from it.

Editors and Affiliations

  • Department of Microbiology & Immunology and Department of Medicine/Hematology Oncology, Indiana University School of Medicine, Indianapolis, USA

    Louis M. Pelus

  • Moderna Therapeutics, Cambridge, USA

    Jonathan Hoggatt

Bibliographic Information

  • Book Title: Hematopoietic Stem Cells

  • Book Subtitle: Methods and Protocols

  • Editors: Louis M. Pelus, Jonathan Hoggatt

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-2679-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2023

  • Hardcover ISBN: 978-1-0716-2678-8Published: 19 October 2022

  • Softcover ISBN: 978-1-0716-2681-8Published: 19 October 2023

  • eBook ISBN: 978-1-0716-2679-5Published: 18 October 2022

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 282

  • Number of Illustrations: 8 b/w illustrations, 55 illustrations in colour

  • Topics: Stem Cells, Immunology, Hematology

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