Skip to main content
SpringerLink
Log in

Fluorescent Proteins

Methods and Protocols

  • Book
  • © 2023

Overview

Editors:
  1. Mayank Sharma

    1. Institute of Molecular Plant Biology, ETH Zürich, Zürich, Switzerland

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2564)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (17 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Using FPbase: The Fluorescent Protein Database

    • Talley J. Lambert
    Pages 1-45

  3. Selecting the Fluorescent Protein for In Vivo Imaging Experiments

    • Mayank Sharma
    Pages 47-52

  4. Advanced Fluorescence Microscopy Methods to Study Dynamics of Fluorescent Proteins In Vivo

    • Marcela Díaz, Leonel Malacrida
    Pages 53-74

  5. Directed Evolution of Fluorescent Proteins in Bacteria

    • Sara Mattson, Geraldine N. Tran, Erik A. Rodriguez
    Pages 75-97

  6. Fluorescent Proteins: Crystallization, Structural Determination, and Nonnatural Amino Acid Incorporation

    • Rochelle D. Ahmed, Husam Sabah Auhim, Harley L. Worthy, D. Dafydd Jones
    Pages 99-119

  7. Development and Characterization of Flavin-Binding Fluorescent Proteins, Part I: Basic Characterization

    • Alina Remeeva, Anna Yudenko, Vera V. Nazarenko, Oleg Semenov, Anastasia Smolentseva, Andrey Bogorodskiy et al.
    Pages 121-141

  8. Development and Characterization of Flavin-Binding Fluorescent Proteins, Part II: Advanced Characterization

    • Nora Lisa Bitzenhofer, Fabienne Hilgers, Gabriela N. Bosio, Joaquim Torra, Giorgia Casini, Felix R. M. Beinlich et al.
    Pages 143-183

  9. CRISPR-Cas9-Mediated Knock-In Approach to Insert the GFP11 Tag into the Genome of a Human Cell Line

    • Ryo Tamura, Daichi Kamiyama
    Pages 185-201

  10. Fluorescent Protein-Based Approaches for Subcellular Protein Localization in Plants

    • Mayank Sharma, Ralf Bernd Klösgen, Bationa Bennewitz
    Pages 203-211

  11. Growth Rate Evaluation of the Budding Yeast Saccharomyces cerevisiae Cells Carrying Endogenously Expressed Fluorescent Protein Fusions

    • Kara L. Schneider, Lucas E. Reibenspies, Thomas Nyström, Sviatlana Shashkova
    Pages 213-222

  12. DNA Visualization Using Fluorescent Proteins

    • Xuelin Jin, Y. Tehee Kim, Kyubong Jo
    Pages 223-246

  13. Quantification of Intracellular Citrate Concentrations with Genetically Encoded Biosensors

    • Yufeng Zhao, Kei Takahashi-Yamashiro, Yi Shen, Robert E. Campbell
    Pages 247-258

  14. Quantification of Redox-Sensitive GFP Cysteine Redox State via Gel-Based Read-Out

    • Finja Bohle, Andreas J. Meyer, Stefanie J. Mueller-Schuessele
    Pages 259-268

  15. Assessing In Vivo Oxygen Dynamics Using Plant N-Terminal Degrons in Saccharomyces cerevisiae

    • Mikel Lavilla-Puerta, Beatrice Giuntoli
    Pages 269-286

  16. Osmium-Resistant Fluorescent Proteins and In-Resin Correlative Light-Electron Microscopy of Epon-Embedded Mammalian Cultured Cells

    • Isei Tanida, Junji Yamaguchi, Soichiro Kakuta, Yasuo Uchiyama
    Pages 287-297

  17. Fluorescence-Detection Size-Exclusion Chromatography-Based Thermostability Assay for Membrane Proteins

    • Hebang Yao, Hongmin Cai, Dianfan Li
    Pages 299-315

  18. Determining Protein-Protein Interaction with GFP-Trap Beads

    • Irem Yilmazer, Melanie R. Abt, Yuanyuan Liang, David Seung, Samuel C. Zeeman, Mayank Sharma
    Pages 317-323

  19. Back Matter

    Pages 325-326
    Download chapter PDF
Back to top

Keywords

About this book

This volume brings together cutting-edge laboratory protocols to characterize the novel fluorescent proteins (FPs) and approaches based on fluorescent proteins that aim to answer some of the key cell biological questions. The book covers topics ranging from the database of fluorescent proteins to their characterization and adaptation to a wide range of biological systems. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Fluorescent Proteins: Methods and Protocols serves as an ideal guide for students and academicians enthusiastic about the recent progress in the practical application of fluorescent protein technology.

Editors and Affiliations

  • Institute of Molecular Plant Biology, ETH Zürich, Zürich, Switzerland

    Mayank Sharma

Bibliographic Information

  • Book Title: Fluorescent Proteins

  • Book Subtitle: Methods and Protocols

  • Editors: Mayank Sharma

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-2667-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2023

  • Hardcover ISBN: 978-1-0716-2666-5Published: 16 September 2022

  • Softcover ISBN: 978-1-0716-2669-6Published: 17 September 2023

  • eBook ISBN: 978-1-0716-2667-2Published: 15 September 2022

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 326

  • Number of Illustrations: 15 b/w illustrations, 80 illustrations in colour

  • Topics: Chemistry/Food Science, general, Cell Biology

Publish with us

Policies and ethics

Back to top

Search

Navigation