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Alzheimer’s Disease

Methods and Protocols

  • Book
  • © 2023

Overview

Editors:
  1. Jerold Chun

    1. Translational Neuroscience Initiative, Neuroscience Drug Discovery, Sanford Burnham Prebys Medical Discovery Institute, La Jolla, USA

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2561)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-xiii
    Download chapter PDF

  2. Human Brain Preparations

    1. Front Matter

      Pages 1-1
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    2. Protocol for the Systematic Fixation, Circuit-Based Sampling, and Qualitative and Quantitative Neuropathological Analysis of Human Brain Tissue

      • Caitlin S. Latimer, Erica J. Melief, Jeanelle Ariza-Torres, Kim Howard, Amanda R. Keen, Lisa M. Keene et al.
      Pages 3-30

    3. Extraction and Purification of Single Nuclei from Frozen Human Brain Tissue

      • Carter R. Palmer, Jerold Chun
      Pages 31-42

    4. Isolation of Human Microglia from Neuropathologically Diagnosed Cases in the Single-Cell Era

      • Lih-Fen Lue, Douglas G. Walker, Suet Theng Beh, Thomas G. Beach
      Pages 43-62

    5. Microglia in Human Postmortem Brain Samples: Quantitative Ultrastructural Analysis of Scanning Electron Microscopy Images

      • Marie-Kim St-Pierre, Eva Šimončičová, Micaël Carrier, Marie-Ève Tremblay
      Pages 63-85

    6. Three-Dimensional Imaging of Fibrinogen and Neurovascular Alterations in Alzheimer’s Disease

      • Mario Merlini, Elif G. Sozmen, Keshav S. Subramanian, Alissa L. Nana, William W. Seeley, Katerina Akassoglou
      Pages 87-101

  3. Human Neural Cellular Models

    1. Front Matter

      Pages 103-103
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    2. Human-Induced Pluripotent Stem Cell (hiPSC)-Derived Neurons and Glia for the Elucidation of Pathogenic Mechanisms in Alzheimer’s Disease

      • Jessica E. Young, Lawrence S. B. Goldstein
      Pages 105-133

    3. Modeling Alzheimer’s Disease Using Human Brain Organoids

      • Karina Karmirian, Mariana Holubiec, Livia Goto-Silva, Ivan Fernandez Bessone, Gabriela Vitória, Beatriz Mello et al.
      Pages 135-158

    4. Three-Dimensional Biohybrid StarPEG–Heparin Hydrogel Cultures for Modeling Human Neuronal Development and Alzheimer’s Disease Pathology

      • Tohid Siddiqui, Hilal Celikkaya, Zeynep Tansu Atasavum, Stanislava Popova, Uwe Freudenberg, Carsten Werner et al.
      Pages 159-170

  4. Nucleic Acid Analyses of Human Brain

    1. Front Matter

      Pages 171-171
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    2. Computational Approaches to Assess Abnormal Metabolism in Alzheimer’s Disease Using Transcriptomics

      • Hatice Büşra Lüleci, Dilara Uzuner, Tunahan Çakır, Madhav Thambisetty
      Pages 173-189

    3. FISHing for Chromosome Instability and Aneuploidy in the Alzheimer’s Disease Brain

      • Yuri B. Yurov, Svetlana G. Vorsanova, Ivan Y. Iourov
      Pages 191-204

    4. Somatic CNV Detection by Single-Cell Whole-Genome Sequencing in Postmortem Human Brain

      • Diego Perez-Rodriguez, Maria Kalyva, Catherine Santucci, Christos Proukakis
      Pages 205-230

  5. Lipids Analyses of Human Brain

    1. Front Matter

      Pages 231-231
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    2. Mass Spectrometry Analysis of the Human Brain Sphingolipidome

      • Xin Ying Chua, Ryan Huang, Deron Herr, Mitchell K. P. Lai, Markus R. Wenk, Federico Torta
      Pages 233-243

    3. Mass Spectrometry for the Advancement of Lipid Analysis in Alzheimer’s Research

      • Jonatan Martínez-Gardeazabal, Marta Moreno-Rodríguez, Estíbaliz González de San Román, Beatriz Abad, Iván Manuel, Rafael Rodríguez-Puertas
      Pages 245-259

  6. Protein Analyses of Human Brain

    1. Front Matter

      Pages 261-261
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    2. Use of Affinity Purification–Mass Spectrometry to Identify Phosphorylated Tau Interactors in Alzheimer’s Disease

      • Geoffrey Pires, Beatrix Ueberheide, Thomas Wisniewski, Eleanor Drummond
      Pages 263-277
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Keywords

About this book

This volume explores the latest techniques used to study the human brain towards understanding Alzheimer’s Disease and related neurodegenerative disorders. Contributed to by world-renowned experts, the chapters in this book are divided into five parts. Part One discusses human post-mortem brain preparations including single-cell isolation and use of specialized imaging. Part Two talks about neural cellular models using primary and human induced pluripotent stem cells to model aspects of the human brain. Part Three details nucleic acid analyses including transcriptomic and somatic genomic changes, and Part Four discusses lipid analyses via mass spectrometry. Lastly, Part Five covers protein analyses, particularly Aβ and Tau. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, andtips on troubleshooting and avoiding known pitfalls.


Cutting-edge and thorough, Alzheimer’s Disease: Methods and Protocols is a valuable tool for all researchers who want to expand their knowledge and understanding of this disease and other related dementias.


Editors and Affiliations

  • Translational Neuroscience Initiative, Neuroscience Drug Discovery, Sanford Burnham Prebys Medical Discovery Institute, La Jolla, USA

    Jerold Chun

Bibliographic Information

  • Book Title: Alzheimer’s Disease

  • Book Subtitle: Methods and Protocols

  • Editors: Jerold Chun

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-2655-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2023

  • Hardcover ISBN: 978-1-0716-2654-2Published: 19 November 2022

  • Softcover ISBN: 978-1-0716-2657-3Published: 19 November 2023

  • eBook ISBN: 978-1-0716-2655-9Published: 18 November 2022

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 340

  • Number of Illustrations: 2 b/w illustrations, 59 illustrations in colour

  • Topics: Neurosciences

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