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Monoamine Oxidase

Methods and Protocols

  • Book
  • © 2023

Overview

Editors:
  1. Claudia Binda

    1. Department of Biology and Biotechnology, University of Pavia, Pavia, Italy

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2558)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. Purification of MAO A and MAO B from Mammalian Tissue Sources

    • Dale E. Edmondson
    Pages 1-10

  3. Purification of Recombinant Eukaryotic MAO A and MAO B Utilizing the Pichia pastoris Expression System

    • Dale E. Edmondson
    Pages 11-22

  4. The Peroxidase-Coupled Assay to Measure MAO Enzymatic Activity

    • Joana Reis, Claudia Binda
    Pages 23-34

  5. Measurement of MAO Enzymatic Activity by Spectrophotometric Direct Assays

    • Joana Reis, Claudia Binda
    Pages 35-43

  6. Radiochemical Assay of Monoamine Oxidase Activity

    • Andrew Holt
    Pages 45-61

  7. MAO Visible Spectroscopy for Ligand Interactions, Redox Chemistry, and Kinetics of Irreversible Inhibition

    • Rona R. Ramsay
    Pages 63-74

  8. Conventional Receptor Radioligand Binding Techniques Applied to the Study of Monoamine Oxidase

    • Andrew Holt
    Pages 75-96

  9. Assay of MAO Inhibition by Chromatographic Techniques (HPLC/HPLC-MS)

    • Tomás Herraiz
    Pages 97-114

  10. Crystallization of Human Monoamine Oxidase B

    • Claudia Binda, Dale E. Edmondson, Andrea Mattevi
    Pages 115-122

  11. Detecting Monoamine Oxidase A and B Proteins: A Western Blotting Protocol and Some Practical Considerations

    • Jennifer N. K. Nyarko, Ryan M. Heistad, Paul R. Pennington, Darrell D. Mousseau
    Pages 123-141

  12. An (Immuno) Fluorescence Protocol for Monitoring Monoamine Oxidase A/B Protein Distribution Within the Cell

    • Tyler J. Wenzel, Jennifer N. K. Nyarko, Ryan M. Heistad, Paul R. Pennington, Chris P. Phenix, Darrell D. Mousseau
    Pages 143-161

  13. Expression and Function of MAO A in Cardiac Cells by Means of Adenovirus-Mediated Gene Transfer

    • Yohan Santin, Angelo Parini, Jeanne Mialet-Perez
    Pages 163-170

  14. In Vitro and In Vivo Assays Characterizing MAO A Function in Cancers

    • Boyang Jason Wu, Jean C. Shih
    Pages 171-182

  15. In Vivo Study of Monoamine Oxidases Using Multisite Intracerebral Microdialysis

    • Philippe De Deurwaerdère, Nouhad Samb, Hasna El Boukhari, Rémi Corne, Abdeslam Chagraoui, Giuseppe Di Giovanni
    Pages 183-195

  16. Informed Use of 3D-QSAR for the Rational Design of Coumarin Derivatives as Potent and Selective MAO B Inhibitors

    • Nicola Gambacorta, Marco Catto, Leonardo Pisani, Angelo Carotti, Orazio Nicolotti
    Pages 197-205

  17. Hansch-Type QSAR Models for the Rational Design of MAO Inhibitors: Basic Principles and Methodology

    • Leonardo Pisani, Modesto de Candia, Mariagrazia Rullo, Cosimo D. Altomare
    Pages 207-220

  18. Computational Chemistry and Molecular Modeling of Reversible MAO Inhibitors

    • Kemal Yelekçi, Safiye SaÄŸ Erdem
    Pages 221-252

  19. Back Matter

    Pages 253-254
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Keywords

About this book

This detailed book examines the main methods to study mammalian monoamine oxidases (MAOs), ranging from cell biology to computational chemistry. Beginning with techniques on how to obtain pure samples of MAO A and MAO B, the volume continues by covering assays and techniques used to measure MAO enzymatic activity and perform inhibition studies, methods to address cellular localization and function of MAOs, either in cell lines or in animal models, as well as computational methods applied to rational drug design approaches that are used to develop new MAO inhibitors. Written for the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Monoamine Oxidase: Methods and Protocols serves as a vitalresource for scientists who are interested in studying MAOs and other similar amine oxidase enzymes.

Editors and Affiliations

  • Department of Biology and Biotechnology, University of Pavia, Pavia, Italy

    Claudia Binda

Bibliographic Information

  • Book Title: Monoamine Oxidase

  • Book Subtitle: Methods and Protocols

  • Editors: Claudia Binda

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-2643-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2023

  • Hardcover ISBN: 978-1-0716-2642-9Published: 29 September 2022

  • Softcover ISBN: 978-1-0716-2645-0Published: 02 October 2023

  • eBook ISBN: 978-1-0716-2643-6Published: 28 September 2022

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 254

  • Number of Illustrations: 35 b/w illustrations, 56 illustrations in colour

  • Topics: Neurochemistry, Enzymology, Pharmaceutical Sciences/Technology

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