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Protein Aggregation

Methods and Protocols

  • Book
  • © 2023

Overview

Editors:
  1. Andrzej Stanisław Cieplak

    1. Cambridge, USA

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2551)

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Table of contents (39 protocols)

  1. Front Matter

    Pages i-xvi
    Download chapter PDF

  2. Early Aggregation of Amyloid-β(1–42) Studied by Fluorescence Correlation Spectroscopy

    • Mercedes Novo, Cibrán Pérez-González, Sonia Freire, Wajih Al-Soufi
    Pages 1-14

  3. Preparation and Investigation of Crucial Oligomers in the Early Stages of Aβ40 and Aβ42 Aggregation

    • Bertrand Morel, Francisco Conejero-Lara
    Pages 15-28

  4. Preparation and Fractionation of Heterogeneous Aβ42 Oligomers with Different Aggregation Properties

    • Erica W. Chen, Zhefeng Guo
    Pages 29-39

  5. An Efficient Method of Expression and Purification of Amyloid-Beta (Aβ1–42) Peptide from E. coli

    • Dhiman Ghosh, Marielle Aulikki Wälti, Roland Riek
    Pages 41-51

  6. Solid-State NMR Structure of Amyloid-β Fibrils

    • Beat H. Meier, Anja Böckmann
    Pages 53-62

  7. Time-Resolved In Situ AFM Measurement of Growth Rates of Aβ40 Fibrils

    • Peter G. Vekilov, Peter G. Wolynes
    Pages 63-77

  8. Monitoring Kinetics of pH-Dependent Aggregation and Disaggregation of the Pmel17 Repeat Domain

    • Dexter N. Dean, Jennifer C. Lee
    Pages 79-93

  9. Analysis of Tau/Nucleoporin Interactions by Surface Plasmon Resonance Spectroscopy

    • Lisa Diez, Larisa E. Kapinos, Roderick Y. H. Lim, Susanne Wegmann
    Pages 95-109

  10. Microfluidic Chamber Technology to Study Missorting and Spreading of Tau Protein in Alzheimer’s Disease

    • Senthilvelrajan Kaniyappan, Varun Balaji, Yipeng Wang, Eckhard Mandelkow
    Pages 111-123

  11. Using FRET-Based Biosensor Cells to Study the Seeding Activity of Tau and α-Synuclein

    • Katherine N. Maina, Caroline Smet-Nocca, Gal Bitan
    Pages 125-145

  12. Functional Applications of Stable Tau Oligomers in Cell Biology and Electrophysiology Studies

    • Emily Hill, Kevin G. Moffat, Mark J. Wall, Henrik Zetterberg, Kaj Blennow, Thomas K. Karikari
    Pages 147-161

  13. An Additive-Free Model for Tau Self-Assembly

    • Youssra K. Al-Hilaly, Karen E. Marshall, Liisa Lutter, Luca Biasetti, Kurtis Mengham, Charles R. Harrington et al.
    Pages 163-188

  14. Cross-Linking Mass Spectrometry Analysis of Metastable Compact Structures in Intrinsically Disordered Proteins

    • Dailu Chen, Lukasz A. Joachimiak
    Pages 189-201

  15. A Validated Method to Prepare Stable Tau Oligomers

    • Emily Hill, Kevin G. Moffat, Mark J. Wall, Henrik Zetterberg, Kaj Blennow, Thomas K. Karikari
    Pages 203-224

  16. Light Microscopy and Dynamic Light Scattering to Study Liquid-Liquid Phase Separation of Tau Proteins In Vitro

    • Janine Hochmair, Christian Exner, Christian Betzel, Eckhard Mandelkow, Susanne Wegmann
    Pages 225-243

  17. Study of Tau Liquid-Liquid Phase Separation In Vitro

    • Solomiia Boyko, Witold K. Surewicz
    Pages 245-252

  18. Liquid-Liquid Phase Separation to Study the Association of Proteins in Solution

    • Irving E. Vega, Andrew Umstead
    Pages 253-267

  19. Mapping Phase Diagram of Tau-RNA LLPS Under Live Cell Coculturing Conditions

    • Yanxian Lin, Yann Fichou, Jennifer N. Rauch, Xuemei Zhang, Kenneth S. Kosik, Songi Han
    Pages 269-284

  20. The Role of Buffers in Wild-Type HEWL Amyloid Fibril Formation Mechanism: A Methodological Approach

    • Sandi Brudar, Barbara Hribar-Lee
    Pages 285-296
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Keywords

About this book

The volume details techniques, methods, and conceptual developments to further the study of protein aggregation with emphasis on the pleiomorphic proteins implicated in etiology of neurodegeneration. Chapters guide readers through in vitro and in vivo studies of fibrillization and liquid-liquid phase separation processes, and offer a comprehensive account of the state-of-art of structural studies of protein aggregation. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. 
Authoritative and cutting-edge, Protein Aggregation: Methods and Protocols aims to be useful and practical guide to new researchers and experts looking to expand their knowledge.

Editors and Affiliations

  • Cambridge, USA

    Andrzej Stanisław Cieplak

Bibliographic Information

  • Book Title: Protein Aggregation

  • Book Subtitle: Methods and Protocols

  • Editors: Andrzej StanisÅ‚aw Cieplak

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-2597-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2023

  • Hardcover ISBN: 978-1-0716-2596-5Published: 31 October 2022

  • Softcover ISBN: 978-1-0716-2599-6Published: 31 October 2023

  • eBook ISBN: 978-1-0716-2597-2Published: 30 October 2022

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVI, 681

  • Number of Illustrations: 30 b/w illustrations, 20 illustrations in colour

  • Topics: Chemistry/Food Science, general, Spectroscopy/Spectrometry

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