Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains keynotes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 2550)
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Table of contents (47 protocols)
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Front Matter
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Methods for the Detection of Melatonin and Its Biological Derivatives
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Front Matter
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Methods to Measure Melatonin Effects at the Molecular and Cellular Level
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Front Matter
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Keywords
About this book
This volume covers the latest techniques that study the synthesis of melatonin, its receptor function, and its effects at the cellular and systemic level. The chapters are organized into three parts. Part One describes methods for the detection of melatonin and its biological derivatives in various biological samples, the manipulation of melatonin synthesis by the pineal gland in animals, and the principal source of melatonin in mammals. Part Two explores methods to measure the biological effects and consequences of melatonin binding to high-affinity G protein-coupled receptors. Part Three describes methods to measure the physiological effects that are regulated by melatonin in animals, particularly in rodent models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, Melatonin: Methods and Protocols is a valuable resource for any researcher interested in investigating melatonin, from its production to its mechanisms of action and systemic effects.
Editors and Affiliations
Bibliographic Information
Book Title: Melatonin
Book Subtitle: Methods and Protocols
Editors: Ralf Jockers, Erika Cecon
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-0716-2593-4
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2022
Hardcover ISBN: 978-1-0716-2592-7Published: 01 October 2022
Softcover ISBN: 978-1-0716-2595-8Published: 02 October 2023
eBook ISBN: 978-1-0716-2593-4Published: 30 September 2022
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XIV, 500
Number of Illustrations: 34 b/w illustrations, 101 illustrations in colour
Topics: Physiology, Neurosciences