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Electrophysiological Analysis of Synaptic Transmission

  • Book
  • © 2022

Overview

Editors:
  1. Nicholas Graziane

    1. Department of Anesthesiology, The Pennsylvania State University, Hershey, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Yan Dong

    1. Neuroscience Department, University of Pittsburgh, Pittsburgh, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Neuromethods (NM, volume 187)

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Table of contents (24 protocols)

  1. Front Matter

    Pages i-xv
    Download chapter PDF

  2. Basic Concepts

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Extracellular and Intracellular Recordings

      • Nicholas Graziane, Yan Dong
      Pages 3-15

    3. Electrical Theory

      • Nicholas Graziane, Yan Dong
      Pages 17-32

    4. Amplifiers

      • Nicholas Graziane, Yan Dong
      Pages 33-53

    5. Salt Environment

      • Nicholas Graziane, Yan Dong
      Pages 55-68

    6. Patch Pipettes (Micropipettes)

      • Nicholas Graziane, Yan Dong
      Pages 69-78

    7. Spatiotemporal Effects of Synaptic Current

      • Nicholas Graziane, Yan Dong
      Pages 79-89

    8. Perforated Patch

      • Nicholas Graziane, Yan Dong
      Pages 91-97

  3. Recording of Synaptic Current

    1. Front Matter

      Pages 99-99
      Download chapter PDF

    2. Isolation of Synaptic Current

      • Nicholas Graziane, Yan Dong
      Pages 101-110

    3. Fast and Slow Synaptic Currents

      • Nicholas Graziane, Yan Dong
      Pages 111-121

    4. Measuring Kinetics of Synaptic Current

      • Nicholas Graziane, Yan Dong
      Pages 123-133

    5. Measuring Presynaptic Release Probability

      • Nicholas Graziane, Yan Dong
      Pages 135-145

    6. Long-Term Measurements

      • Nicholas Graziane, Yan Dong
      Pages 147-158

    7. Measuring Reversal Potentials

      • Nicholas Graziane, Yan Dong
      Pages 159-163

  4. Basic Experimentations of Synaptic Transmission

    1. Front Matter

      Pages 165-165
      Download chapter PDF

    2. Amplitude

      • Nicholas Graziane, Yan Dong
      Pages 167-176

    3. Pre-vs. Post-synaptic Effect

      • Nicholas Graziane, Yan Dong
      Pages 177-188

    4. Run-Up and Run-Down

      • Nicholas Graziane, Yan Dong
      Pages 189-194
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Keywords

About this book

This second edition volume expands on the previous edition with discussions on the latest techniques used to study synaptic transmissions.  The chapters in this book are organized into six parts. Part One looks at the basic concepts, such as extracellular and intracellular recordings, and spatiotemporal effects of synaptic currents. Part Two describes the recording of synaptic currents, such as measuring kinetics of synaptic current and measuring reversal potentials. Part Three discusses basic experimentations of synaptic transmission and covers run-up and run-down, and amplitude. Parts Four and Five cover experimentations with computational components and molecular and visual components, such as measurement of a single synapse and electrophysiological and visual tags. Part Six explores in vivo recordings and talks about general considerations for in vivo exploration of synaptic plasticity. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. 

Authoritative and thorough, Electrophysiological Analysis of Synaptic Transmission, Second Edition is a valuable resource that introduces graduate students and postdoctoral fellows to important topics in this field and also expands these topics to practical electrophysiological approaches. 


Editors and Affiliations

  • Department of Anesthesiology, The Pennsylvania State University, Hershey, USA

    Nicholas Graziane

  • Neuroscience Department, University of Pittsburgh, Pittsburgh, USA

    Yan Dong

About the editors

The two authors, Dr. Nicholas Graziane and Dr. Yan Dong, each have over 10 years of experience in the field of electrophysiology. Dr. Graziane started his research career in 2003 and he continues to perform electrophysiologically relevant research as an assistant professor at Penn State College of Medicine. Dr. Dong had been running an active electro- physiology laboratory and teaching electrophysiology for ~15 years. The partnership of these two authors was specifically designed to provide a student-friendly electrophysiology guide that explains complicated concepts in a straightforward manner. More importantly, the authors address the most common errors that occur during electrophysiological measurements and the most straightforward approaches that can be implemented to correct these errors. In this second edition volume, Drs. Graziane and Dong have teamed up with Dr. Xiyu Zhu and Dr. Anthony Grace in order to provide a comprehensive description of in vivo electrophysiological applications. Dr. Anthony Grace has over 40 years of published experience using in vivo electrophysiology, has published more than 350 articles (H index 111) and is an expert in recording from midbrain dopamine neurons and their relevant corticolimbic structures. Dr. Xiyu Zhu is a trainee in the Dr. Grace laboratory with over 5 years of experience in in vivo recording techniques, whose expertise have been significantly contributing to several research articles. It is the hope of the authors that their efforts will guide and enrich the knowledge of an aspiring electrophysiologist with a passion for neuroscience. 

Bibliographic Information

  • Book Title: Electrophysiological Analysis of Synaptic Transmission

  • Editors: Nicholas Graziane, Yan Dong

  • Series Title: Neuromethods

  • DOI: https://doi.org/10.1007/978-1-0716-2589-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2022

  • Hardcover ISBN: 978-1-0716-2588-0Published: 04 August 2022

  • Softcover ISBN: 978-1-0716-2591-0Published: 04 August 2023

  • eBook ISBN: 978-1-0716-2589-7Published: 03 August 2022

  • Series ISSN: 0893-2336

  • Series E-ISSN: 1940-6045

  • Edition Number: 2

  • Number of Pages: XV, 289

  • Number of Illustrations: 45 b/w illustrations, 46 illustrations in colour

  • Topics: Neurosciences

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