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DNA-Encoded Chemical Libraries

Methods and Protocols

  • Book
  • © 2022

Overview

Editors:
  1. David Israel

    1. HitGen Inc, Chengdu, China

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    You can also search for this editor in PubMed   Google Scholar

  2. Yun Ding

    1. R&D Medicinal Science and Technology, GlaxoSmithKline, Cambridge, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2541)

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Table of contents (25 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. Library Synthesis: Building Block Selection, Handling, and Tracking

    • Katelyn J. Billings, Melissa C. Grenier-Davies
    Pages 1-11

  3. Library Synthesis: Building Block Validation

    • Melissa C. Grenier-Davies
    Pages 13-16

  4. Library Synthesis and Analytics

    • Jing Chai
    Pages 17-24

  5. Chemical Ligation of Oligonucleotide Tags to Support Encoded Chemical Library Synthesis

    • Alexander Litovchick, Anthony D. Keefe
    Pages 25-32

  6. On-DNA Reductive Amination and Alkylation

    • Jing Chai
    Pages 33-37

  7. On-DNA-1,2,3-Triazole Formation via Click Reaction

    • Sarah K. Scott
    Pages 39-43

  8. On-DNA Suzuki-Miyaura Cross-Coupling

    • Yun Ding
    Pages 45-48

  9. On-DNA Pd and Cu-Promoted C-N Cross-Coupling Reactions

    • Xiaojie Lu, Xuan Wang
    Pages 49-54

  10. Palladium-Promoted On-DNA Heck Reactions

    • Xuan Wang, Xiaojie Lu
    Pages 55-61

  11. Ruthenium-Promoted On-DNA C-H Activation Reaction

    • Xuan Wang, Xiaojie Lu
    Pages 63-66

  12. DNA-Compatible Nitro Reduction and Synthesis of Benzimidazoles

    • Huang-Chi Du, Ying-Chu Chen, Hongbing Huang
    Pages 67-73

  13. Condensation of DNA-Conjugated Imines with Homophthalic Anhydride for the Synthesis of Isoquinolones On-DNA

    • Josephine Yuen, Jing Chai, Yun Ding
    Pages 75-79

  14. DNA Encoding of Natural Products

    • Hongtao Xu, Peixiang Ma
    Pages 81-87

  15. Initiating DNA-Encoded Library Synthesis with a Hexathymidine DNA Oligonucleotide

    • Mateja Klika Å kopić, Denise dos Santos Smith, Anne Gohla, Verena B. K. Kunig, Andreas Brunschweiger
    Pages 89-104

  16. One Bead-One Compound (OBOC) Peptidomimetic-Encoded Library Synthesis via Split-and-Pool Methods

    • Claudio Aquino, Mohosin Sarkar
    Pages 105-120

  17. DNA Conjugates as Tool Compounds for DEL Selections

    • David I. Israel
    Pages 121-133

  18. Quantitation of DNA-Encoded Libraries by qPCR

    • Christopher S. Kollmann
    Pages 135-142

  19. Target Protein Design and Preselection Analysis

    • Qiuxia Chen, David I. Israel
    Pages 143-154

  20. DEL Selections Against a Soluble Protein Target

    • Qiuxia Chen, Junyang Zhu
    Pages 155-164
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Keywords

About this book

This volume discusses protocols that cover synthesis, screening by selection, and analysis of DNA-encoded chemical libraries (DEL). Chapters in this book focus on methods used to practice DEL technology and include solution phase library synthesis using a variety of chemistries; DNA encoding of chemical structure; design, preparation and analysis of target proteins and tool compounds; screening of soluble protein targets by affinity selection; DEL qPCR, preparative PCR and DNA sequence analysis; computational methods used to analyze selections and choose compounds for resynthesis; and analysis of hit compounds. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, DNA-Encoded Chemical Libraries:Methods and Protocols is a valuable resource for scientists interested in DEL technology for drug discovery, and will contribute to the continued advancement in this important field.



Editors and Affiliations

  • HitGen Inc, Chengdu, China

    David Israel

  • R&D Medicinal Science and Technology, GlaxoSmithKline, Cambridge, USA

    Yun Ding

Bibliographic Information

  • Book Title: DNA-Encoded Chemical Libraries

  • Book Subtitle: Methods and Protocols

  • Editors: David Israel, Yun Ding

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-2545-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2022

  • Hardcover ISBN: 978-1-0716-2544-6Published: 10 September 2022

  • Softcover ISBN: 978-1-0716-2547-7Published: 11 September 2023

  • eBook ISBN: 978-1-0716-2545-3Published: 09 September 2022

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 226

  • Number of Illustrations: 12 b/w illustrations, 64 illustrations in colour

  • Topics: Genetics and Genomics

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