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Drosophila

Methods and Protocols

  • Book
  • © 2022

Overview

Editors:
  1. Christian Dahmann

    1. Institute of Genetics, Technische Universität Dresden, Dresden, Germany

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2540)

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Table of contents (21 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Using FlyBase: A Database of Drosophila Genes and Genetics

    • Victoria K. Jenkins, Aoife Larkin, Jim Thurmond, The FlyBase Consortium
    Pages 1-34

  3. The Q-system: A Versatile Repressible Binary Expression System

    • Orsolya Fölsz, Chun-Chieh Lin, Darya Task, Olena Riabinina, Christopher J. Potter
    Pages 35-78

  4. Resources and Methods for the Analysis of MicroRNA Function in Drosophila

    • Sromana Mukherjee, Nicholas Sokol
    Pages 79-92

  5. Analysis of Single-Cell Transcriptome Data in Drosophila

    • Schayan Yousefian, Maria Jelena Musillo, Josephine Bageritz
    Pages 93-111

  6. Prime Editing for Precise Genome Engineering in Drosophila

    • Justin A. Bosch, Norbert Perrimon
    Pages 113-134

  7. CRISPR-/Cas9-Mediated Precise and Efficient Genome Editing in Drosophila

    • Kevin G. Nyberg, Richard W. Carthew
    Pages 135-156

  8. Tissue-Specific CRISPR-Cas9 Screening in Drosophila

    • Fillip Port, Michael Boutros
    Pages 157-176Open Access

  9. CRISPR-Based Transcriptional Activation in Drosophila

    • Yuting Han, Xinyi Lu, Yutong Li, Yuhao Qiu, Xizhi Dong, Xiaochen Li et al.
    Pages 177-199

  10. Tracing and Manipulating Drosophila Cell Lineages Based on CRISPR: CaSSA and CLADES

    • Jorge Garcia-Marques, Tzumin Lee
    Pages 201-217

  11. Studying Protein Function Using Nanobodies and Other Protein Binders in Drosophila

    • Katarzyna Lepeta, Milena Bauer, Gustavo Aguilar, M. Alessandra Vigano, Shinya Matsuda, Markus Affolter
    Pages 219-237

  12. Anchor Away: A System for Fast Inhibition of Proteins in Drosophila

    • Pablo Sanchez Bosch
    Pages 239-249

  13. Tagging Drosophila Proteins with Genetically Encoded Fluorophores

    • Jerome Avellaneda, Frank Schnorrer
    Pages 251-268

  14. Optogenetic Methods to Control Tissue Mechanics in Drosophila

    • Daniel Krueger, Stefano De Renzis
    Pages 269-283

  15. Optochemical Control of Cell Contractility in Drosophila Embryos

    • Deqing Kong, Jörg Großhans
    Pages 285-299

  16. Stiffness Measurement of Drosophila Egg Chambers by Atomic Force Microscopy

    • Uwe Töpfer, Karla Yanín Guerra Santillán, Elisabeth Fischer-Friedrich
    Pages 301-315

  17. Cultivation and Live Imaging of Drosophila Imaginal Discs

    • Natalie A. Dye
    Pages 317-334

  18. Sample Preparation and Imaging of the Pupal Drosophila Abdominal Epidermis

    • Daiki Umetsu
    Pages 335-347

  19. FlyClear: A Tissue-Clearing Technique for High-Resolution Microscopy of Drosophila

    • Marko Pende, Saiedeh Saghafi, Klaus Becker, Thomas Hummel, Hans-Ulrich Dodt
    Pages 349-359

  20. Preparation of Drosophila Tissues and Organs for Transmission Electron Microscopy

    • Olympia-Ekaterini Psathaki, Achim Paululat
    Pages 361-385
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Keywords

About this book

This third edition expands on the previous editions by providing updated protocols and current methods that cover recent breakthroughs in Drosophila research. Chapters guide readers through FlyBase, versatile gene expression systems, analysis of microRNA function, single-cell transcriptome data and metabolism, recent applications of CRISPR for precise genome editing, transcriptional activation and cell lineage tracing, protein inhibition and tagging, optogenetic and optochemical control of tissue mechanics, AFM measurements, sample preparation for electron microscopy, live imaging of different tissues and organs, and quantitative image analysis. Written in the format of the highly successful Methods in Molecular Biology series, chapters include an introduction to their topic, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls

 Authoritative and cutting-edge, Drosophila: Methods and Protocols, Third Edition serves as a useful and practical guide to new researchers and experts using Drosophila as a model system.

Chapter 7 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Editors and Affiliations

  • Institute of Genetics, Technische Universität Dresden, Dresden, Germany

    Christian Dahmann

Bibliographic Information

  • Book Title: Drosophila

  • Book Subtitle: Methods and Protocols

  • Editors: Christian Dahmann

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-2541-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2022

  • Hardcover ISBN: 978-1-0716-2540-8Published: 19 August 2022

  • Softcover ISBN: 978-1-0716-2543-9Published: 20 August 2023

  • eBook ISBN: 978-1-0716-2541-5Published: 18 August 2022

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 3

  • Number of Pages: XI, 424

  • Number of Illustrations: 5 b/w illustrations, 69 illustrations in colour

  • Topics: Biological Techniques

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