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Optical Tweezers

Methods and Protocols

  • Book
  • © 2022

Overview

Editors:
  1. Arne Gennerich

    1. Department of Biochemistry, Albert Einstein College of Medicine, Bronx, USA

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2478)

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Table of contents (26 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Historic Views on the Invention of Lasers and Optical Tweezers

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. The Invention of the Laser

      • Nick Taylor
      Pages 3-10

    3. Art Ashkin and the Origins of Optical Trapping and Particle Manipulation

      • Matthias D. Koch, Joshua W. Shaevitz
      Pages 11-22

  3. Technical Advances

    1. Front Matter

      Pages 23-23
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    2. Synthesis of Germanium Nanospheres as High-Precision Optical Tweezers Probes

      • Swathi Sudhakar, Pasupathi Rajendran, Erik Schäffer
      Pages 25-35

    3. Angular Optical Trapping to Directly Measure DNA Torsional Mechanics

      • Xiang Gao, James T. Inman, Michelle D. Wang
      Pages 37-73

    4. Implementation of 3D Multi-Color Fluorescence Microscopy in a Quadruple Trap Optical Tweezers System

      • Anna E. C. Meijering, Julia A. M. Bakx, Tianlong Man, Iddo Heller, Gijs J. L. Wuite, Erwin J. G. Peterman
      Pages 75-100

    5. One-Dimensional STED Microscopy in Optical Tweezers

      • Tianlong Man, Joost J. Geldhof, Erwin J. G. Peterman, Gijs J. L. Wuite, Iddo Heller
      Pages 101-122

    6. Temperature Quantification and Temperature Control in Optical Tweezers

      • Joost J. Geldhof, Agata M. Malinowska, Gijs J. L. Wuite, Erwin J. G. Peterman, Iddo Heller
      Pages 123-140

    7. High-Resolution Optical Tweezers Combined with Multicolor Single-Molecule Microscopy

      • Rajeev Yadav, Kasun B. Senanayake, Matthew J. Comstock
      Pages 141-240

  4. DNA, DNA Motors and DNA–Protein Interactions

    1. Front Matter

      Pages 241-241
      Download chapter PDF

    2. Generating Negatively Supercoiled DNA Using Dual-Trap Optical Tweezers

      • Graeme A. King, Dian Spakman, Erwin J. G. Peterman, Gijs J. L. Wuite
      Pages 243-272

    3. Force-Activated DNA Substrates for In Situ Generation of ssDNA and Designed ssDNA/dsDNA Structures in an Optical-Trapping Assay

      • Arnulf M. K. Taylor, Stephen R. Okoniewski, Lyle Uyetake, Thomas T. Perkins
      Pages 273-312

    4. Probing the Interaction Between Chromatin and Chromatin-Associated Complexes with Optical Tweezers

      • Rachel Leicher, Shixin Liu
      Pages 313-327

    5. Simultaneous Mechanical and Fluorescence Detection of Helicase-Catalyzed DNA Unwinding

      • Lulu Bi, Zhenheng Qin, Xi-Miao Hou, Mauro Modesti, Bo Sun
      Pages 329-347

    6. CRISPR/Cas9 On- and Off-Target Activity Using Correlative Force and Fluorescence Single-Molecule Microscopy

      • Matthew D. Newton, Benjamin J. Taylor, Maria Emanuela Cuomo, David S. Rueda
      Pages 349-378
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Keywords

About this book

This detailed volume explores a wide variety of techniques involving optical tweezers, a technology that has become increasingly more accessible to a broad range of researchers. Beginning with recent technical advances, the book continues by covering the application of optical tweezers to study DNA-protein interactions and DNA motors, protocols to perform protein (un)folding experiments, the application of optical tweezers to study actin- and microtubule-associated motor proteins, and well as protocols for investigating the function and mechanical properties of microtubules and intermediate filaments, and more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Optical Tweezers: Methodsand Protocols, Second Edition serves as an ideal resource for expanding the accessibility and use of optical traps by scientists of diverse disciplines.

Editors and Affiliations

  • Department of Biochemistry, Albert Einstein College of Medicine, Bronx, USA

    Arne Gennerich

Bibliographic Information

  • Book Title: Optical Tweezers

  • Book Subtitle: Methods and Protocols

  • Editors: Arne Gennerich

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-2229-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2022

  • Hardcover ISBN: 978-1-0716-2228-5Published: 06 September 2022

  • Softcover ISBN: 978-1-0716-2231-5Published: 07 September 2023

  • eBook ISBN: 978-1-0716-2229-2Published: 05 September 2022

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XIV, 757

  • Number of Illustrations: 21 b/w illustrations, 174 illustrations in colour

  • Topics: Biological and Medical Physics, Biophysics, Optics, Lasers, Photonics, Optical Devices

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