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CRISPR-Cas Methods

Volume 2

  • Book
  • © 2021

Overview

Editors:
  1. M. Tofazzal Islam

    1. Institute of Biotechnology and Genetic Engineering (IBGE), Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur, Bangladesh

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    You can also search for this editor in PubMed   Google Scholar

  2. Kutubuddin Ali Molla

    1. ICAR-National Rice Research Institute, Cuttack, India

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Springer Protocols Handbooks (SPH)

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Table of contents (26 protocols)

  1. Front Matter

    Pages i-xvii
    Download chapter PDF

  2. A Complete Methodology for the Instruction of CRISPR-Based Gene Editing Using a Simplified Cell-Free Extract System with Genetic Readout in Bacteria

    • Kristen M. Pisarcik, Brett M. Sansbury, Eric B. Kmiec
    Pages 1-13

  3. In Silico Analysis of gRNA Secondary Structure to Predict Its Efficacy for Plant Genome Editing

    • Md Mahmudul Hassan, Abul Kashem Chowdhury, Tofazzal Islam
    Pages 15-22

  4. In Vitro Cas9 Cleavage Assay to Check Guide RNA Efficiency

    • Subhasis Karmakar, Deeptirekha Behera, Mirza Jaynul Baig, Kutubuddin A. Molla
    Pages 23-39

  5. Efficient Multiplexed CRISPR-Cas12a Genome Editing in Plants

    • Yingxiao Zhang, Yiping Qi
    Pages 41-56

  6. CRISPR-dCas9-Based Targeted Manipulation of DNA Methylation in Plants

    • Basudev Ghoshal, Jason Gardiner
    Pages 57-71

  7. Rapid Assembly of Multiplex Natural CRISPR Arrays

    • Robert M. Cooper, Jeff Hasty
    Pages 73-81

  8. Assembly and Assessment of Prime Editing Systems for Precise Genome Editing in Plants

    • Simon Sretenovic, Changtian Pan, Yiping Qi
    Pages 83-101

  9. Designing, Performing, and Analyzing CRISPR-Cas9-Mediated Genome Editing Experiments in Leguminous Plants

    • Dipak Gayen, Subhasis Karmakar
    Pages 103-122

  10. Generation of Knockout and Fragment Deletion Mutants in Soybean by CRISPR-Cas9

    • Li Chen, Yupeng Cai, Wensheng Hou
    Pages 123-135

  11. Targeted Base Editing in Soybean Using a CRISPR-Cas9 Cytidine Deaminase Fusion

    • Li Chen, Yupeng Cai, Wensheng Hou
    Pages 137-148

  12. Efficient Genome Editing in Potato Using a Hairy Root Transformation System

    • Nathaniel M. Butler, Jiming Jiang
    Pages 149-158

  13. Efficient CRISPR-Cas9-Mediated Genome Editing in Tomato

    • Viswanathan Satheesh, Jinkai Li, Mingguang Lei
    Pages 159-177

  14. CRISPR-Cas9-Mediated Genome Editing in Rice: A Systematic Protocol for Single- and Multi-Target Vector Construction

    • Hirendra Nath Barman
    Pages 179-205

  15. Generating Clonal Seeds from Hybrid Rice with CRISPR-Cas9

    • Chaolei Liu, Chun Wang, Kejian Wang
    Pages 207-219

  16. CRISPR-Cas12a-Based DNA Detection for Fast Pathogen Diagnosis and GMO Test in Plants

    • Yun-Mu Zhang, Yinong Yang, Kabin Xie
    Pages 221-233

  17. CRISPR-Cas9-Mediated Gene Editing in Human Induced Pluripotent Stem Cells

    • Stijn L. M. in ’t Groen, Mike Broeders, W. W. M. Pim Pijnappel
    Pages 235-264Open Access

  18. Cell-Type-Specific CRISPR-Cas9 System with miRNAs

    • Moe Hirosawa, Hirohide Saito
    Pages 265-279

  19. CRISPR/Cas9 Gene Editing in Mammalian Cells Using LentiCRISPRv2/LentiGuide-Puro Vectors

    • Zubaida Sa’id Ameen, Ece Cakiroglu, Serif Senturk, Abdullahi Umar Ibrahhim, Mehmet Ozsoz
    Pages 281-299

  20. An Approach to Proximity Ligation by T4 RNA Ligase to Screen sRNA That Regulate CRISPR-Cas Systems

    • Ping Lin, Qinqin Pu, Min Wu
    Pages 301-309
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Keywords

About this book

This second volume provides new and updated methods detailing advancements in CRISPR-Cas technical protocols. Chapters guide readers through protocols on prime editing, base editing, multiplex editing, editing in cell-free extract, in silico analysis of gRNA secondary structure and CRISPR-diagnosis. 

 Authoritative and cutting-edge,  CRISPR-Cas Methods, Volume 2 aims to serves as a laboratory manual providing scientists with a  holistic view of CRISPR-Cas methodologies and its practical application for the editing of crop plants, cell lines, nematode and microorganism.


The chapter “CRISPR/Cas9-mediated gene editing in human induced pluripotent stem cells” is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Editors and Affiliations

  • Institute of Biotechnology and Genetic Engineering (IBGE), Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur, Bangladesh

    M. Tofazzal Islam

  • ICAR-National Rice Research Institute, Cuttack, India

    Kutubuddin Ali Molla

Bibliographic Information

  • Book Title: CRISPR-Cas Methods

  • Book Subtitle: Volume 2

  • Editors: M. Tofazzal Islam, Kutubuddin Ali Molla

  • Series Title: Springer Protocols Handbooks

  • DOI: https://doi.org/10.1007/978-1-0716-1657-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-1656-7Published: 31 July 2021

  • Softcover ISBN: 978-1-0716-1659-8Published: 31 July 2022

  • eBook ISBN: 978-1-0716-1657-4Published: 30 July 2021

  • Series ISSN: 1949-2448

  • Series E-ISSN: 1949-2456

  • Edition Number: 1

  • Number of Pages: XVII, 397

  • Number of Illustrations: 12 b/w illustrations, 106 illustrations in colour

  • Topics: Genetics and Genomics

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