Overview
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 323)
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About this book
For several decades, Arabidopsis thaliana has been the organism of choice in the laboratories of many plant geneticists, physiologists, developmental biologists, and biochemists around the world. During this time, a huge amount of knowledge has been acquired on the biology of this plant species, which has resulted in the development of molecular tools that account for much more efficient research. The significance that Arabidopsis would attain in biological research may have been difficult to foresee in the 1980s, when its use in the laboratory started. In the meantime, it has become the model plant organism, much the same way as Drosophila, Caenorhabditis, or mouse have for animal systems. Today, it is difficult to envision research at the cutting edge of plant biology without the use of Arabidopsis. Since the first edition of Arabidopsis Protocols appeared, new developments have fostered an impressive advance in plant biology that prompted us to prepare Arabidopsis Protocols, Second Edition. Completion of the Arabidopsis genome sequence offered for the first time the opportunity to have in hand all of the genetic information required for studying plant function. In addition, the development of whole systems approaches that allow global analysis of gene expression and protein and metabolite dynamics has encouraged scientists to explore new scenarios that are extending the limits of our knowledge.
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Keywords
Table of contents (37 protocols)
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Front Matter
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Genetic Analyses
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Front Matter
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Transient and Stable Transformation
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Front Matter
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Reviews
From the reviews of the first edition:
". . .a comprehensive collection of significant new methods as well as updated standard protocols. . .written by experienced authors and internationally recognized experts."-Journal of Plant Physiology
"...recommend[ed]...provides a set of enduring methods and is comprehensive enough for instant use in the lab" -Trends in Plant Science
"...a 'must have' for every laboratory involved in Arabibopsis" -Quarterly Review of Biology
"...a 'must' for those labs working with not just Arabidopsis specifically but plants in general..." -Microbiology Today
"...a must for anybody moving into the Arabidopsis field and setting up a new lab...extremely useful" -Genetical Research
"...especially valuable for detailed step-by-step procedures including many important notes...an indispensable guide, which should be at reach on the shelf in each laboratory where the plant molecular-genetic methods in Arabidopsis, and not only Arabidopsis, are used."- Biologia Plantarum
"...should prove to be highly helpful laboratory companion for beginners and established researchers in molecular biology, particularly those whose research deal with Arabidopsis and related plants." - Plant Science Bulletin
From the reviews of the second edition:
"Arabidopsis Protocols is another volume in Humana’s series, Methods in Molecular Biology. … It is … important to provide both experienced researchers and those new to the use of Arabidopsis, with single volumes that describe a wide range of experimental protocols. This book does that admirably. It is written by renowned experts in their respective fields … . Most chapters include useful Figures to illustrate the text. So I would heartily recommend this to any lab serious about carrying out work with Arabidopsis … ." (Keith Lindsey, Molecular Biotechnology, Vol. 36, 2007)
Editors and Affiliations
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Bibliographic Information
Book Title: Arabidopsis Protocols, 2nd Edition
Editors: Julio Salinas, Jose J. Sanchez-Serrano
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1385/1597450030
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Humana Press 2006
Hardcover ISBN: 978-1-58829-395-4Published: 15 March 2006
Softcover ISBN: 978-1-61737-539-2Published: 09 December 2010
eBook ISBN: 978-1-59745-003-4Published: 04 February 2008
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 2
Number of Pages: 496
Number of Illustrations: 135 b/w illustrations, 1 illustrations in colour
Topics: Popular Science in Nature and Environment, Plant Sciences