Overview
- Comprehensive account of cutting-edge, virological techniques with a wide range of protocols
- Written by well-known HIV researchers selected for their expertise in the respective methodologies
- Covers both virological and immunological techniques related to HIV in a single volume
- Conveniently arranged protocols studying different aspects of viral replication reflect the order in which the events take place in the viral life cycle
- Provides a breadth of coverage of many critical steps in HIV replication that could serve as targets for drug therapy
Part of the book series: Methods in Molecular Biology (MIMB, volume 485)
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About this book
Despite major advances in HIV treatment, many areas require more study, in order to create efficacious, potent antiretrovirals that can suppress viral load completely and durably without toxic side effects, to define unknown drug targets and fine-tune known targets, and to better understand the interplay between viral and host factors. In "HIV Protocols, Second Edition", expert researchers provide clear, state-of-the-art methods for the study of HIV. Directed toward three specific goals, this text aims to document up-to-date protocols for select aspects of HIV biology, to bring together both virological and immunological approaches in a single, convenient volume, and to present a comprehensive account of a range of techniques not available in any existing HIV protocol book. As a volume in the highly successful Methods in Molecular Biology™ series, the chapters include brief introductions to the subject, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections containing priceless tips on troubleshooting and avoiding known pitfalls.
Comprehensive and cutting-edge, "HIV Protocols, Second Edition" is an ideal guide to the wide array of techniques used in fundamental or applied research into the biology and pathogenesis of HIV-1.
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Keywords
Table of contents (30 protocols)
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Front Matter
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Preparation of Virus Particles and their Analysis
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Front Matter
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Methods To Study Hiv-1 Replication
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Front Matter
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Early Events
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Late Events
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Specialized Approaches To Study Hiv-1 Biology and Pathogenesis
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Front Matter
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Editors and Affiliations
Bibliographic Information
Book Title: HIV Protocols
Book Subtitle: Second Edition
Editors: Vinayaka R. Prasad, Ganjam V. Kalpana
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-59745-170-3
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Humana Press 2009
Hardcover ISBN: 978-1-58829-859-1Published: 08 December 2008
Softcover ISBN: 978-1-61737-808-9Published: 19 November 2010
eBook ISBN: 978-1-59745-170-3Published: 20 November 2008
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 2
Number of Pages: XIV, 457
Topics: Infectious Diseases, Immunology, Virology