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Methods in Rhizosphere Biology Research

  • Book
  • © 2019

Overview

Editors:
  1. Didier Reinhardt

    1. Department of Biology, University of Fribourg, Fribourg, Switzerland

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  2. Anil K. Sharma

    1. Biological Sciences, CBSH, G.B.Pant University of Agriculture & Technology, Pantnagar, India

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Provides complete and comprehensive experimental procedures
  • Offers not only experimental methods, but also the theoretical background of each method
  • Includes abundant references, examples, useful figures and trouble shooting guides

Part of the book series: Rhizosphere Biology (RHBIO)

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Table of contents (15 chapters)

  1. Front Matter

    Pages i-ix
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  2. Root Symbioses

    1. Front Matter

      Pages 1-1
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    2. Synthetic Plasmids to Challenge Symbiotic Nitrogen Fixation Between Rhizobia and Legumes

      • Jovelyn Unay, Xavier Perret
      Pages 3-18

    3. In Vivo Analysis of Rhizosphere Enzyme Activities by the Use of Plastic Syringes

      • Ru-Jie Li, Yi-Han Wang, Jie Cai, Jie Liu, Zhi-Ping Xie, Christian Staehelin
      Pages 19-25

    4. Characterization of Arbuscular Mycorrhizal Communities in Roots of Vineyard Plants

      • Alice Drain, Laurent Bonneau, Ghislaine Recorbet, Diederik van Tuinen, Daniel Wipf, Pierre-Emmanuel Courty
      Pages 27-34

    5. Molecular Methods for Research on Actinorhiza

      • Hassen Gherbi, Valérie Hocher, Mariama Ngom, Nathalie Diagne, Joëlle Fournier, Alyssa Carre-Mlouka et al.
      Pages 35-59

    6. Molecular and Functional Characterization of Beneficial Bacteria Associated with AMF Spores

      • Monica Agnolucci, Alessandra Turrini, Manuela Giovannetti
      Pages 61-79

  3. Plant Pathogens and Microbial Plant Protection

    1. Front Matter

      Pages 81-81
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    2. Oomycete-Root Interactions

      • Jacob Hargreaves, Pieter van West
      Pages 83-103

    3. Exploitation of Rhizosphere Microbiome Services

      • Valentina Riva, Elisa Terzaghi, Lorenzo Vergani, Francesca Mapelli, Elisabetta Zanardini, Cristiana Morosini et al.
      Pages 105-132

    4. Methods for Detecting Biocontrol and Plant Growth-Promoting Traits in Rhizobacteria

      • Gustavo Santoyo, Juan M. Sánchez-Yáñez, Sergio de los Santos-Villalobos
      Pages 133-149

    5. A Split-Root Method to Study Systemic and Heritable Traits Induced by Trichoderma in Tomato Plants

      • M. B. Rubio, H. A. de Medeiros, M. E. Morán-Diez, P. Castillo, R. Hermosa, E. Monte
      Pages 151-166

  4. Experimental Approaches and Analytical Techniques in Rhizosphere Biology

    1. Front Matter

      Pages 167-167
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    2. Using a Tri-Isotope (13C, 15N, 33P) Labelling Method to Quantify Rhizodeposition

      • Pierre Stevenel, E. Frossard, S. Abiven, I. M. Rao, F. Tamburini, A. Oberson
      Pages 169-195

    3. Microscopic Techniques Coupled to Molecular and Genetic Approaches to Highlight Cell-Type Specific Differences in Mycorrhizal Symbiosis

      • Valentina Fiorilli, Veronica Volpe, Raffaella Balestrini
      Pages 197-225

    4. From Imaging to Functional Traits in Interactions Between Roots and Microbes

      • Yukari Kuga, Klaus Schläppi, Didier Reinhardt
      Pages 227-239

    5. Live Imaging of Arbuscular Mycorrhizal Symbiosis

      • Yoshihiro Kobae
      Pages 241-253

    6. Microcosm Approaches to Investigate Multitrophic Interactions between Microbial Communities in the Rhizosphere of Plants

      • Michael Bonkowski
      Pages 255-270

    7. Analysis of Common Mycorrhizal Networks in Microcosms

      • Laurent Bonneau, Ghislaine Recorbet, Diederik van Tuinen, Daniel Wipf, Pierre-Emmanuel Courty
      Pages 271-279
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Keywords

About this book

This book compiles various methodologies used in understanding interactions within the rhizosphere. An in-depth understanding of the rhizosphere is essential to developing successful strategies for future sustainable agriculture. The book summarizes methods and techniques used to study the mechanisms involved in mutualistic symbioses and pathogenic interactions of plants with various microbial organisms including fungi, bacteria, and oomycetes. Each chapter discusses different methodologies used in rhizosphere biology, while also providing real-world experimental data and trouble-shooting tips. Interested researchers will also find a wealth of literature references for further research. As the first comprehensive manual and compilation of methods and techniques used in rhizosphere biology, the book represents an essential resource for all researchers who are newcomers to soil microbiology experimentation. 

Editors and Affiliations

  • Department of Biology, University of Fribourg, Fribourg, Switzerland

    Didier Reinhardt

  • Biological Sciences, CBSH, G.B.Pant University of Agriculture & Technology, Pantnagar, India

    Anil K. Sharma

About the editors

Prof. D. Reinhardt is a researcher at the University of Fribourg, Switzerland. After completing his doctoral studies in the field of plant-pathogen interactions at the University of Basel, and a postdoc stay at the Salk Institute in La Jolla (California), he worked at the University of Bern. His main area of interest encompasses various aspects of arbuscular mycorrhizal symbiosis, with particular emphasis on the genetic factors in the host required for the intracellular accommodation of the fungal symbiont, and on the regulation of AM by exogenous factors such as nutrient availability. In addition, Prof. Reinhardt is currently investigating organ formation and regulation of the body plan of plants. This includes the positioning of leaves and flowers around the growth axis, a phenomenon known as phyllotaxis. He has published over 50 research and review articles in various scientific journals. 


Prof. Anil Kumar Sharma is a Professor at the Department ofBiological Sciences, CBSH G.B. of Pant University of Agriculture & Technology in Pantnagar. He completed his postdoctoral studies at the GSU, Louisiana (USA), and has been a Visiting Scientist at the University of Basel, Switzerland (2003) and University of Helsinki, Finland (2013). He has extensive research and teaching experience, and served as a reviewer for various funding bodies and international research journals. He also holds two patents in plant biology and microbiology, respectively. The recipient of various prestigious grants, he has published more than 60 research articles and 30 review articles, as well as two books with prominent publishers.

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