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Engineering a Robust DNA Circuit for the Direct Detection of Biomolecular Interactions

  • Offers readers essential insights into designing a localized DNA circuit to promote the rate of desired reaction over undesired leak reactions in the bulk solution
  • Proposes design concepts for individual reaction modules, with a focus on minimizing circuit leakage
  • Covers applications for the biomolecular sensing of a broad range of targets including the mutation of nucleic acids, protein, and cell surface receptors

Part of the book series: Springer Theses (Springer Theses)

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About this book

This book provides essential insights into designing a localized DNA circuit to promote the rate of desired hybridization reactions over undesired leak reactions in the bulk solution. The area of dynamic DNA nanotechnology, or DNA circuits, holds great promise as a highly programmable toolbox that can be used in various applications, including molecular computing and biomolecular detection. However, a key bottleneck is the recurring issue of circuit leakage. The  assembly of the localized circuit is dynamically driven by the recognition of biomolecules – a different approach from most methods, which are based on a static DNA origami assembly. The design guidelines for individual reaction modules presented here, which focus on minimizing circuit leakage, are established through NUPACK simulation and tested experimentally – which  will be useful for researchers interested in adapting the concepts for other contexts. In the closing section, the design concepts are successfully applied to the biomolecular sensing of a broad range of targets including the single nucleotide mutations, proteins, and cell surface receptors. 

Authors and Affiliations

  • Department of Chemical and Biomolecular Engineering, National University of Singapore, Singapore, Singapore

    Ang Yan Shan

Bibliographic Information

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access