Authors:
- Clear step-by-step description of the full range of methods
- Advice on fundamentals and prerequisites
- Guidance on avoidance of errors
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Table of contents (7 chapters)
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Front Matter
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Back Matter
About this book
This book offers step-by-step instruction on DNA cloning, defined as moving genes around plasmids, mutating genes, or mining new genes. The aim is to provide those new to the field with reliable and up-to-date practical guidance while at the same time conveying the scope for creativity. After a brief synopsis of the history of cloning, the fundamentals and prerequisites are explained, covering, for example, software, vectors commonly used in the lab, appropriate choice of restriction endonucleases, the preparation of agarose gels, competent cells, and LB agar plates, and procedures to be followed upon receipt of new plasmids. The remainder of the book is devoted to the clear description of methods and individual steps in cloning. Guidance is provided on the cut and paste method, DNA sequencing, direct sequencing, primer design, PCR-based gene insertion and deletion, epitope tag insertion, the use of RACE technology, BAC recombineering, and much, much more. Sources of error and a variety of techniques that make life considerably easier when cloning are also examined in detail.
Authors and Affiliations
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Department of Biomedical Sciences, Chonnam National University Medical School, Gwangju, Korea (Republic of)
Seok-Yong Choi
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Department of Biological Sciences, Chungnam National University, Daejeon, Korea (Republic of)
Hyunju Ro, Hankuil Yi
About the authors
Hyunju Ro, Department of Biological Sciences, Chungnam National University, Daejeon, Republic of Korea
Hankuil Yi, Department of Biological Sciences, Chungnam National University, Daejeon, Republic of Korea
Bibliographic Information
Book Title: DNA Cloning: A Hands-on Approach
Authors: Seok-Yong Choi, Hyunju Ro, Hankuil Yi
DOI: https://doi.org/10.1007/978-94-024-1662-6
Publisher: Springer Dordrecht
eBook Packages: Biomedical and Life Sciences, Biomedical and Life Sciences (R0)
Copyright Information: Springer Nature B.V. 2019
Hardcover ISBN: 978-94-024-1660-2Published: 14 May 2019
eBook ISBN: 978-94-024-1662-6Published: 17 April 2019
Edition Number: 1
Number of Pages: XIII, 131
Number of Illustrations: 23 b/w illustrations, 70 illustrations in colour
Topics: Gene Expression, Genetic Engineering, Plant Genetics and Genomics, Animal Genetics and Genomics