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Plant Molecular Biology Manual

  • Book
  • © 1994

Overview

Editors:
  1. Stanton B. Gelvin

    1. Purdue University, West Lafayette, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. Robbert A. Schilperoort

    1. Leiden State University, Leiden, The Netherlands

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    You can also search for this editor in PubMed   Google Scholar

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Table of contents (37 chapters)

  1. Front Matter

    Pages i-xix
    Download chapter PDF

  2. In vitro methods of gene transfer to plant cells

    1. PEG-mediated direct gene transfer and electroporation

      • Roland Bilang, Andreas Klöti, Martin Schrott, Ingo Potrykus
      Pages 1-16

    2. Gene transfer to plants via particle bombardment

      • Paul Christou
      Pages 17-31

  3. Agrobacterium-mediated gene transfer to plant cells

    1. Agrobacterium-mediated gene transfer to plant cells: cointegrate and binary vector systems

      • Cindy R. Walkerpeach, Jeff Velten
      Pages 33-51

    2. Specialized vectors for gene tagging and expression studies

      • Csaba Koncz, Norbert Martini, László Szabados, Milan Hrouda, Andreas Bachmair, Jeff Schell
      Pages 53-74

    3. Agrobacterium molecular genetics

      • Paul J. J. Hooykaas, Teresa Mozo
      Pages 75-83

    4. Genetic manipulation of Agrobacterium tumefaciens strains to improve transformation of recalcitrant plant species

      • Stanton B. Gelvin, Chang-Nong Liu
      Pages 85-97

    5. Transient expression assays using GUS constructs and fluorometric detection for analysis of T-DNA transfer

      • Linda A. Castle, Roy O. Morris
      Pages 99-114

    6. Agrobacterium inoculation techniques for plant tissues

      • Nancy L. Mathis, Maud A. W. Hinchee
      Pages 115-123

  4. Selectable and screenable markers for plant transformation

    1. Antibiotic resistance markers for plant transformation

      • Geert Angenon, Willy Dillen, Marc Van Montagu
      Pages 125-137

    2. Reporter genes for plants

      • Luis Herrera-Estrella, Patricia León, Olof Olsson, H. Teemu Teeri
      Pages 139-170

    3. Opines as screenable markers for plant transformation

      • Yves Dessaux, Annik Petit
      Pages 171-182

  5. Nucleic acid extraction from plant tissue

    1. Extraction of total cellular DNA from plants, algae and fungi

      • Scott O. Rogers, Arnold J. Bendich
      Pages 183-190

    2. Isolation and characterization of nuclear scaffolds

      • Gerald E. Hall Jr., Steven Spiker
      Pages 191-202

    3. Isolation of plant mitochondria and mitochondrial nucleic acids

      • Sally A. Mackenzie
      Pages 203-214

    4. Isolation of chloroplasts and chloroplast DNA

      • C. A. Price, Noureddine Hadjeb, Lee Newman, Ellen M. Reardon
      Pages 215-229

    5. Isolation of total, poly(A) and polysomal RNA from plant tissues

      • Katharina Pawlowski, Reinhard Kunze, Sacco De Vries, Ton Bisseling
      Pages 231-243

  6. Transcription and translation systems

    1. Assay for gene expression using run-on transcription in isolated nuclei

      • Imre E. Somssich
      Pages 245-255

    2. Preparation of an in vitro transcription system of plant origin, with methods and templates for assessing its fidelity

      • Yuhki Yamaguchi, Fujio Mukumoto, Hidemasa Imaseki, Ken-Ichi Yamazaki
      Pages 257-271

  7. Blotting and gene detection systems

    1. Southern, Northern and Western blot analysis

      • Johan Memelink, Kathleen M. M. Swords, L. Andrew Staehelin, J. Harry C. Hoge
      Pages 273-295
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Keywords

About this book

Five years ago, the first edition of the Plant Molecular Biology Manual appeared. At that time, the editors felt that the field of plant molecular biology had matured to a point that the publication of a series of protocols in plant molecular biology was warranted. During the past five years, the field of plant molecular biology has expanded rapidly. This expansion is, among other things, reflected by the presence of several journals in the plant sciences, as well as by the increasing amount of plant sciences articles that are published in the more general journals. In 1991 approximately 3000 people attended the Third International Congress of Plant Molecular Biology in Tucson, Arizona, where more than 2000 posters were presented. It is also remarkable to see that nowadays botanical and physiological meetings pay a considerable amount of attention to plant molecular biology. Since the first edition of this manual appeared, we have published, yearly, a series of supplements to the original volume. These supplements covered new subjects and described new methods that had been developed. With time, however, the editors realized that the original manual plus supplements had become cumbersome to use, and we decided to publish a reorganized version of the manual.

Editors and Affiliations

  • Purdue University, West Lafayette, USA

    Stanton B. Gelvin

  • Leiden State University, Leiden, The Netherlands

    Robbert A. Schilperoort

Bibliographic Information

  • Book Title: Plant Molecular Biology Manual

  • Editors: Stanton B. Gelvin, Robbert A. Schilperoort

  • DOI: https://doi.org/10.1007/978-94-011-0511-8

  • Publisher: Springer Dordrecht

  • eBook Packages: Springer Book Archive

  • Copyright Information: Kluwer Academic Publishers 1994

  • Softcover ISBN: 978-94-011-7654-5Published: 13 November 2012

  • eBook ISBN: 978-94-011-0511-8Published: 06 December 2012

  • Edition Number: 1

  • Number of Pages: XIX, 598

  • Topics: Biochemistry, general, Science, Humanities and Social Sciences, multidisciplinary

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