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Techniques for the Analysis of Membrane Proteins

  • Book
  • © 1986

Overview

Editors:
  1. C. Ian Ragan

    1. Department of Biochemistry, University of Southampton, UK

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  2. Richard J. Cherry

    1. Department of Chemistry, University of Essex, Colchester, UK

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Table of contents (13 chapters)

  1. Front Matter

    Pages i-xi
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  2. Analysis of membrane protein composition by gel electrophoresis

    • C. Ian Ragan
    Pages 1-25

  3. Analysis of membrane protein composition by isoelectric focusing and two–dimensional electrophoresis

    • Robert B. Freedman, E. N. Clare Mills
    Pages 27-59

  4. Solubilization and purification of membrane proteins

    • Yutaka Yanagita, Yasuo Kagawa
    Pages 61-76

  5. Reconstitution of membrane proteins into vesicular membranes

    • Hajime Hirata
    Pages 77-96

  6. Functional reconstitution of membrane proteins in planar lipid bilayer membranes

    • Mauricio Montal
    Pages 97-128

  7. Topography of membrane proteins — determination of regions exposed to the aqueous phase

    • Paul A. Hargrave
    Pages 129-151

  8. Topology of membrane proteins — determination of regions exposed to the lipid bilayer

    • Roberto Bisson, Cesare Montecucco
    Pages 153-184

  9. Immunochemical analysis of membrane proteins

    • Milton R. J. Salton
    Pages 185-234

  10. Sequence analysis of membrane proteins

    • John E. Walker, Ian M. Fearnley
    Pages 235-274

  11. Freeze—fracture and freeze—etch electron microscopy of membrane proteins

    • Jena K. Khodadad, Jerome M. Loew, Ronald S. Weinstein
    Pages 275-314

  12. Three-dimensional structure of membrane proteins

    • Sven Hovmöller
    Pages 315-344

  13. Lateral motion of membrane proteins

    • Hans-Geurg Kapitza, Kenneth A. Jacobson
    Pages 345-375

  14. Rotational diffusion of membrane proteins

    • David D. Thomas
    Pages 377-431

  15. Back Matter

    Pages 433-441
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Keywords

About this book

A preface should justify the existence of the book it precedes and this is invariably done in scientific texts by reference to the explosive growth of the field since the last such volume appeared. In molecular biology, most fields can be justifiably described as growing explosively, as should be the case for a young and vigorous science, but the study of membrane proteins stands out as one which has taken giant strides in the last few years. Ignorance of the structure and function of membrane proteins at the molecular level was certainly not due to lack of interest but rather was a result of lack of appropriate techniques. It has above all been the development of new experimental methods which has wrenched membrane biochemistry out of what Anthony Martonosi fetchingly called its 'romantic phase' (Le. lots of ideas and few facts), into an era when the determination of membrane protein structure and mechanism is a reasonable goal. Membrane proteins are generally classified as peripheral or integral. Peripheral proteins are relatively easily dissociated from membranes by mild treatments whence their study is essentially no different to that of soluble proteins. This book therefore concentrates on integral proteins which are strongly bound to the membrane by hydrophobic interactions with lipids. A crucial step in their study is of necessity the d~velopment of methods of solubilization and purification under non-denaturing conditions.

Editors and Affiliations

  • Department of Biochemistry, University of Southampton, UK

    C. Ian Ragan

  • Department of Chemistry, University of Essex, Colchester, UK

    Richard J. Cherry

Bibliographic Information

  • Book Title: Techniques for the Analysis of Membrane Proteins

  • Editors: C. Ian Ragan, Richard J. Cherry

  • DOI: https://doi.org/10.1007/978-94-009-4085-7

  • Publisher: Springer Dordrecht

  • eBook Packages: Springer Book Archive

  • Copyright Information: Springer Science+Business Media Dordrecht 1986

  • Softcover ISBN: 978-94-010-8312-6Published: 03 October 2013

  • eBook ISBN: 978-94-009-4085-7Published: 06 December 2012

  • Edition Number: 1

  • Number of Pages: XI, 441

  • Number of Illustrations: 44 b/w illustrations

  • Topics: Science, Humanities and Social Sciences, multidisciplinary

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