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Analyzing T Cell Responses

How to analyze cellular immune responses against tumor associated antigens

  • Book
  • © 2005

Overview

Editors:
  1. Dirk Nagorsen

    1. Charité University Medicine Berlin, Berlin, Germany

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    You can also search for this editor in PubMed   Google Scholar

  2. F.M. Marincola

    1. National Institutes of Health, Bethesda, USA

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    You can also search for this editor in PubMed   Google Scholar

  • Comprehensive review of all methods suitable for T cell responses
  • Prepared by pioneers in each respective assay
  • Inclusive of strategies for global monitoring at the systemic and peripheral (target organ) level

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Table of contents (18 chapters)

  1. Front Matter

    Pages i-xii
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  2. Monitoring Antigen-specific T Cell Responses

    • Dirk Nagorsen, Francesco M. Marincola
    Pages 1-7

  3. Tumor Associated Antigens

    • Paul F. Robbins
    Pages 9-42

  4. Immune Escape

    • Theresa L. Whiteside, Michael Campoli, Soldano Ferrone
    Pages 43-81

  5. Virus Specific T-Cell Responses

    • Victor Appay
    Pages 83-101

  6. Cytotoxicity Assays

    • Gideon Berke, William R. Clark
    Pages 103-121

  7. Monitoring T Cell Proliferation

    • Philip D. Hodgkin, Edwin D. Hawkins, Jhaguaral Hasbold, Amanda V. Gett, Elissa K. Deenick, Hilary F. Todd et al.
    Pages 123-141

  8. ELISPOT Assay

    • Theresa L. Whiteside
    Pages 143-155

  9. Modified ELISPOT

    • Anatoli Malyguine
    Pages 157-174

  10. Intracellular Cytokine Staining

    • Carmen Scheibenbogen, Anne Letsch, Anne Marie Asemissen, Alexander Schmittel, Eckhard Thiel, Ulrich Keilholz
    Pages 175-182

  11. Cytometric Cytokine Secretion Assay

    • Mario Assenmacher
    Pages 183-195

  12. Peptide/MHC Tetramer Analysis

    • Peter P. Lee, Francesco M. Marincola
    Pages 197-217

  13. In Situ MHC Tetramer Staining

    • Pamela J. Skinner
    Pages 219-225

  14. MHC-Ig Dimeric Molecules

    • Tim F. Greten, Firouzeh Korangy
    Pages 227-238

  15. TCR Analyses

    • Markus J. Maeurer
    Pages 239-260

  16. Peptide/HLA-GFP Complexes

    • Utano Tomaru, Yoshihisa Yamano, Steven Jacobson
    Pages 261-274

  17. QRT-PCR

    • Udai S. Kammula
    Pages 275-284

  18. Microarrays

    • Ena Wang
    Pages 285-301

  19. Concluding Remark

    • Dirk Nagorsen, Francesco M. Marincola
    Pages 303-303

  20. Back Matter

    Pages 305-313
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Keywords

About this book

Active specific immunotherapy is a promising but investigational modality in the management of cancer patients. Currently, several different cancer vaccine formulations such as peptides, proteins, antigen-pulsed dendritic cells, whole tumor cells, etc. in combination with various adjuvants and carriers are being evaluated in clinical trials (1-3). To determine the optimal cancer vaccine strategy, a surrogate immunological end-point that correlates with clinical outcome needs to be defined, since it would facilitate the rapid comparison of these various formulations. Traditional immunological assays such as ELISA, proliferation and cytotoxicity assays can detect immune responses in vaccinated patients but are not quantitative. In contrast, novel assays such as enzyme-linked immunospot (ELISPOT) assay, intracellular cytokine assay and tetramer assay can quantitate the frequency of antigen-specific T cells. Of these, the ELISPOT assay has the 5 lowest detection limit with 1/10 peripheral blood mononuclear cells (PBMC) and has been determined to be one of the most useful assays to evaluate immune response to cancer vaccines (4). However, the IFN-? ELISPOT assay is not an exclusive measure of cytotoxic T-lymphocyte (CTL) activity as non-cytotoxic cells can also secrete IFN-?. Additionally, CTL with lytic activity do not always secrete IFN-? (5). A more relevant approach to assess functional activity of cytotoxic lymphocytes would be to measure the secretion of molecules that are associated with lytic activity. One of the major mechanisms of cell-mediated cytotoxicity involves exocytosis of cytoplasmic granules from the effector toward the target cell.

Reviews

From the reviews of the first edition:

"This book represents the first comprehensive description, and evaluation of the most important assays utilized to monitor immune responses against tumor associated antigens. … Specifically tailored to the needs of clinical researchers, basic science investigators, and biotechnology innovators, this book provides information that is both sufficiently detailed and eloquently succinct. All discussions are substantiated with a comprehensive literature review. … Analyzing T Cell Responses provides the most substantial and extensive review of immune response-monitoring techniques available to date." (Melanie Hayden, Stephanie Schroter and Boris Minev, Angiogenesis, Vol. 9, 2006)

Editors and Affiliations

  • Charité University Medicine Berlin, Berlin, Germany

    Dirk Nagorsen

  • National Institutes of Health, Bethesda, USA

    F.M. Marincola

Bibliographic Information

  • Book Title: Analyzing T Cell Responses

  • Book Subtitle: How to analyze cellular immune responses against tumor associated antigens

  • Editors: Dirk Nagorsen, F.M. Marincola

  • DOI: https://doi.org/10.1007/1-4020-3623-X

  • Publisher: Springer Dordrecht

  • eBook Packages: Biomedical and Life Sciences, Biomedical and Life Sciences (R0)

  • Copyright Information: Springer Science+Business Media B.V. 2005

  • Hardcover ISBN: 978-1-4020-3622-4Published: 29 September 2005

  • Softcover ISBN: 978-90-481-6911-5Published: 19 October 2010

  • eBook ISBN: 978-1-4020-3623-1Published: 16 January 2006

  • Edition Number: 1

  • Number of Pages: XII, 314

  • Topics: Immunology, Cancer Research, Hematology, Blood Transfusion Medicine, Virology

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