Animal Cell Technology: Challenges for the 21st Century

1. Front Matter

   Pages i-xiii

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2. Asian Biotechnology in Future

   1. The Role of Hong Kong SAR in the Development of Biotechnology Industry in China

      • Albert Yen Chang

      Pages 1-4

3. Cell Culture and Engineering

   1. Increase in Intracellular Calcium Influx Enhances Monoclonal Antibody Productivity

      • Sun Ho Park, Jae Sung Park, Min Hee Nam

      Pages 5-9

   2. Energy Metabolism and Antibody Production Based on Flux Analysis in Hybridoma

      • Takeshi Omasa, Tomoya Iemura, Keisuke Furuichi, Yoshio Katakura, Michimasa Kishimoto, Ken-ichi Suga

      Pages 11-15

   3. Transient Transfection Induces Early Cytosolic Calcium Signaling in CHO-K1 Cells

      • A. K. Preuss, H. M. Pick, F. Wurm, H. Vogel

      Pages 17-22

   4. An On-Line Feeding Strategy for Fed-Batch and Dialysis Cultures of Hybridoma Cells

      • J. O. Schwabe, R. Pörtner, H. Märkl

      Pages 23-27

   5. Effects of Serum and Other Medium Components on Suspension Culture of CHO Cells Producing Tissue Plasminogen Activator

      • M. Takagi, H. C. Hia, J. H. Jang, T. Yoshida

      Pages 29-32

   6. Effects of Osmotic Pressure on Metabolism of CHO Cells Producing Tissue Plasminogen Activator in Adhesion Culture Compared with that in Suspension Culture

      • M. Takagi, H. Hayashi, T. Yoshida

      Pages 33-38

   7. Cultivation of CHO Cells on Novel Microcarriers

      • A. Y. Hu, Z. Zhang, M. Al-Rubeal

      Pages 39-43

   8. Bleeding Strategy for the Long-Term Perfusion Culture of Hybridoma

      • J. W. Yun, S. Y. Lee, B. W. Choi, H. K. Oh, J. S. Lee, B. H. Chun et al.

      Pages 45-49

   9. Metabolic Flux Distributions in Hybridoma Cells at Different Metabolic States

      • Peng-Cheng Fu, Anna Europa, Anshu Gambhir, Wei-Shou Hu

      Pages 51-55

   10. On-Line Detection of Metabolic Demand and Changes to it: Heat Flux in Continuous Cultures of Animal Cells

       • Y. H. Guan, RB. Kemp

       Pages 57-61

   11. Growth Stimulation of CHO Cells by Phospholipids in Serum-Free Culture

       • Kentaro Sakai, Toshio Matsunaga, Hideki Yamaji, Hideki Fukuda

       Pages 63-67

   12. A Kinetic Model of Macromolecular Metabolism in Batch and Fed-Batch Cultures of Hybridoma Cells Producing Monoclonal Antibody

       • J. D. Jang, J.P Barford

       Pages 69-73

   13. Ehancement of Antibody Productivity in the Near-Zero Specific Growth Rate During Glucose and Glutamine-Limited Fed-Batch Culture of Hybridoma Cells

       • J.D Jang, J.P Barford

       Pages 75-79

   14. Long-Term Culture of CHO Cells on Porous Microcarriers in a Stirred Tank Equipped with a Modified Cell Retention System

       • Xianwen Hu, Chengzu Xiao, Zicai Huang, Zhixia Guo

       Pages 81-85

   15. Continuous production and recovery of recombinant Ca++ binding receptor from HEK 293 cells using perfusion through a packed bed bioreactor

       • J. B. Kaufman, G. Wang, W. Zhang, M. A. Valle, J. Shiloach

       Pages 87-91

4. Production of Biologicals

   1. Characterization of Recombinant Bovine Lactoperoxidase Produced by CHO Cells

      • S. Watanabe, K. Shimazaki, A. Bollen, N. Moguilevsky

      Pages 93-97

   2. Expression of a Soluble αβ Heterodimeric T Cell Receptor in Animal Cells

      • Maho Amano, Mamoru Totsuka, Kiyotaka Fujine, Shinya Yamada, Shuichi Kaminogawa

      Pages 99-103

   3. Posttranslational Modifications of Recombinant Von Willebrand Factor: Limitations and Experimental Improvement at High Yield Expression

      • B. Plaimauer, U. Schlokat, M. Himmelspach, P. L. Turecek, H. P. Schwarz, F. G. Falkner et al.

      Pages 105-109

Animal cell technology is a growing discipline of cell biology which aims not only to understand structures, functions and behaviors of differentiated animal cells but also to ascertain their abilities to be used in industrial and medical purposes. The goal of animal cell technology includes accomplishments of clonal expansion of differentiated cells with useful ability, optimization of their culture conditions, modulation of their ability to produce medically and pharmaceutically, important proteins, and the application of animal cells to gene therapy and artificial organs. This volume gives the readers a complete review of present state of the art in Japan. The Proceedings will be useful for cell biologists, biochemists, molecular biologists, immunologists, biochemical engineers and other disciplines related to animal cell culture, working in either academic environments or in biotechnology and pharmaceutical industries.

• Monoclonal Antibodies
• Transplantation
• biotechnology
• cell biology
• gene therapy
• proteins
• transgen

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