Overview
- Nominated by The University of Tokyo as an outstanding Ph.D. thesis
- Provides a detailed description of the history and applications of aminoacylation ribozymes
- Describes potentials of aminoacylation ribozymes to be applied for further study
- Includes supplementary material: sn.pub/extras
Part of the book series: Springer Theses (Springer Theses)
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Table of contents (4 chapters)
Keywords
About this book
In this thesis, applications of aminoacylation ribozymes named flexizymes are described. Flexizymes have the following unique characteristics: (i) substrate RNA is recognized by two consecutive base pairs between the 3'-end of substrate RNA and the 3'-end of the flexizyme; (ii) these base pairs can be substituted with other base pairs; and (iii) various activated amino acids can be used as substrates including both canonical and noncanonical amino acids. This flexible aminoacylation of RNAs by flexizymes was used to label endogenous tRNAs to be removed, and in vitro selection using the tRNA-depleted library enabled the discovery of the novel interaction between the microRNA precursor and metabolites. Flexizymes are also used to prepare various aminoacyl-tRNAs bearing mutations at the 3'-end to engineer the translation machinery and to develop the orthogonal translation machinery.
The first part of the research demonstrated that SELEX is appropriate for discovering the interaction between small RNA and ligands, and suggested that more RNA motif binding to small molecules exists in small RNAs. The second part opened a door to new opportunities for in vitro synthetic biology involving the engineering of the genetic codes and translation machineries. This research also indicated the great potential of aminoacylation by flexizymes to be applied in various fields of RNA research, which is beneficial for RNA researchers.
Authors and Affiliations
Bibliographic Information
Book Title: Applications of Aminoacylation Ribozymes That Recognize the 3′-end of tRNA
Authors: Naohiro Terasaka
Series Title: Springer Theses
DOI: https://doi.org/10.1007/978-4-431-56517-8
Publisher: Springer Tokyo
eBook Packages: Chemistry and Materials Science, Chemistry and Material Science (R0)
Copyright Information: Springer Japan KK, part of Springer Nature 2017
Hardcover ISBN: 978-4-431-56515-4Published: 17 March 2017
Softcover ISBN: 978-4-431-56809-4Published: 21 July 2018
eBook ISBN: 978-4-431-56517-8Published: 08 March 2017
Series ISSN: 2190-5053
Series E-ISSN: 2190-5061
Edition Number: 1
Number of Pages: XII, 89
Number of Illustrations: 10 b/w illustrations, 28 illustrations in colour
Topics: Bioorganic Chemistry, Catalysis, Nucleic Acid Chemistry, Biotechnology