Authors:
- Nominated by Kyoto University as an outstanding Ph.D. thesis
- Introduces detailed protocols of larger-sized DNA origami assembling and especially photo functionalization
- Presents new methods for the observation of dynamic activities of single biomolecules
- Includes supplementary material: sn.pub/extras
Part of the book series: Springer Theses (Springer Theses)
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Table of contents (5 chapters)
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Front Matter
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Back Matter
About this book
In this book, the author deals mainly with two topics: (1) single-molecule visualization of switching behaviors in the DNA nanoframe system utilizing different kinds of molecular switches through the use of high-speed atomic force microscope (AFM); (2) construction of photocontrollable DNA nanostructures in programmed patterns and direct visualization of the dynamic assembling process. Here, high-speed AFM was employed to observe the dynamic movements of single molecules. Compared to a traditional single-molecule analysis method, such as fluorescence spectroscopy or electron microscopy, high-speed AFM makes possible the real-time observation of molecule behaviors. DNA nanostructures were designed and assembled as scaffolds to incorporate interested biomolecules. The observations were carried out under robust conditions without complicated pretreatment. Moreover, the photoresponsive molecules were successfully assembled into around 100 nm-sized DNA nanostructures. The assembly/disassembly of nanostructures can be regulated reversibly by photoirradiation. This book explains how DNA origami has gradually become a useful tool for the investigation of biochemical interactions in defined nanospace. It also shows the possibility of DNA nanostructures acting as nanodevices for application in biological systems, serving as a good introduction to basic DNA nanotechnology.
Authors and Affiliations
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Kyoto University, Kyoto, Japan
Yangyang Yang
Bibliographic Information
Book Title: Artificially Controllable Nanodevices Constructed by DNA Origami Technology
Book Subtitle: Photofunctionalization and Single-Molecule Analysis
Authors: Yangyang Yang
Series Title: Springer Theses
DOI: https://doi.org/10.1007/978-4-431-55769-2
Publisher: Springer Tokyo
eBook Packages: Chemistry and Materials Science, Chemistry and Material Science (R0)
Copyright Information: Springer Japan 2015
Hardcover ISBN: 978-4-431-55768-5Published: 02 March 2016
Softcover ISBN: 978-4-431-56690-8Published: 07 April 2018
eBook ISBN: 978-4-431-55769-2Published: 16 December 2015
Series ISSN: 2190-5053
Series E-ISSN: 2190-5061
Edition Number: 1
Number of Pages: XIII, 76
Number of Illustrations: 43 b/w illustrations, 1 illustrations in colour
Topics: Nanochemistry, Nanotechnology, Biological Microscopy, Spectroscopy and Microscopy