Skip to main content
SpringerLink
Log in
Birkhäuser
Book cover

A Laboratory Guide to Genomic Sequencing

The Direct Sequencing of Native Uncloned DNA

  • Book
  • © 1987

Overview

Authors:
  1. Hanspeter Saluz

    1. Friedrich Miescher-Institut, Basel, Switzerland

    View author publications

    You can also search for this author in PubMed   Google Scholar

  2. Jean-Pierre Jost

    1. Friedrich Miescher-Institut, Basel, Switzerland

    View author publications

    You can also search for this author in PubMed   Google Scholar

Part of the book series: Biomethods (BIOMETHODS)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 39.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (18 chapters)

  1. Front Matter

    Pages 1-8
    Download chapter PDF

  2. Introduction

    1. Introduction

      • Hanspeter Saluz, Jean-Pierre Jost
      Pages 9-13

  3. Theoretical Background

    1. Theoretical Background

      • Hanspeter Saluz, Jean-Pierre Jost
      Pages 15-22

  4. Expiremental

    1. Front Matter

      Pages 23-23
      Download chapter PDF

    2. Isolation of Genomic DNA

      • Hanspeter Saluz, Jean-Pierre Jost
      Pages 24-32

    3. Restriction Digest of Genomic DNA

      • Hanspeter Saluz, Jean-Pierre Jost
      Pages 33-40

    4. Chemical Sequencing Reactions on Restricted DNA

      • Hanspeter Saluz, Jean-Pierre Jost
      Pages 41-50

    5. Separation of Reaction Products on a Sequencing Gel

      • Hanspeter Saluz, Jean-Pierre Jost
      Pages 51-58

    6. Electrotransfer to Nylon Membranes

      • Hanspeter Saluz, Jean-Pierre Jost
      Pages 59-65

    7. Immobilization of DNA on Nylon Membranes

      • Hanspeter Saluz, Jean-Pierre Jost
      Pages 66-72

    8. Prehybridization and Hybridization of Immobilized DNA with Labeled Single-Stranded DNA Probes

      • Hanspeter Saluz, Jean-Pierre Jost
      Pages 73-84

    9. Processing of the Hybridized Filters

      • Hanspeter Saluz, Jean-Pierre Jost
      Pages 85-89

    10. Autoradiography and Photography

      • Hanspeter Saluz, Jean-Pierre Jost
      Pages 90-98

    11. Cloning of DNA Probe in M13

      • Hanspeter Saluz, Jean-Pierre Jost
      Pages 99-105

    12. Large-Scale Preparation of Cloned DNA in M13

      • Hanspeter Saluz, Jean-Pierre Jost
      Pages 106-110

    13. Synthesis of Oligonucleotide Primers and Single-Stranded Labeled Probes

      • Hanspeter Saluz, Jean-Pierre Jost
      Pages 111-117

    14. Purification of Labeled Single-Stranded Probes

      • Hanspeter Saluz, Jean-Pierre Jost
      Pages 118-124

  5. Trouble-Shooting Guide

    1. Front Matter

      Pages 125-126
      Download chapter PDF

    2. Trouble-Shooting Guide

      • Hanspeter Saluz, Jean-Pierre Jost
      Pages 125-149

  6. Appendix

    1. Appendix

      • Hanspeter Saluz, Jean-Pierre Jost
      Pages 151-156
Back to top

Keywords

Authors and Affiliations

  • Friedrich Miescher-Institut, Basel, Switzerland

    Hanspeter Saluz, Jean-Pierre Jost

Bibliographic Information

  • Book Title: A Laboratory Guide to Genomic Sequencing

  • Book Subtitle: The Direct Sequencing of Native Uncloned DNA

  • Authors: Hanspeter Saluz, Jean-Pierre Jost

  • Series Title: Biomethods

  • DOI: https://doi.org/10.1007/978-3-0348-9302-2

  • Publisher: Birkhäuser Basel

  • eBook Packages: Springer Book Archive

  • Copyright Information: Birkhäuser Verlag Basel 1987

  • Softcover ISBN: 978-3-7643-1925-0Published: 01 January 1987

  • eBook ISBN: 978-3-0348-9302-2Published: 07 March 2013

  • Edition Number: 1

  • Number of Pages: 164

  • Number of Illustrations: 31 b/w illustrations

  • Topics: Science, Humanities and Social Sciences, multidisciplinary

Publish with us

Policies and ethics

Back to top

Search

Navigation