Plant DNA Infectious Agents

1. Front Matter

   Pages I-XIV

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2. The Molecular Biology of Cauliflower Mosaic Virus and Its Application as Plant Gene Vector

   • Bruno Gronenborn

   Pages 1-29

3. The Structure, Expression, Functions and Possible Exploitation of Geminivirus Genomes

   • J. W. Davies, R. Townsend, J. Stanley

   Pages 31-52

4. cDNA Cloning of Plant RNA Viruses and Viroids

   • Pieter Vos

   Pages 53-85

5. Agroinfection

   • Nigel Grimsley, David Bisaro

   Pages 87-107

6. The Mechanism of T-DNA Transfer from Agrobacterium tumefaciens to the Plant Cell

   • Zdena Koukolíková-Nicola, Lisa Albright, Barbara Hohn

   Pages 109-148

7. Molecular Analysis of Root Induction by Agrobacterium rhizogenes

   • Frank F. White, Vilas P. Sinkar

   Pages 149-177

8. Pathways to Plant Genetic Manipulation Employing Agrobacterium

   • Stephen G. Rogers, Harry Klee

   Pages 179-203

9. Plant Transposable Elements: Unique Structures for Gene Tagging and Gene Cloning

   • U. Wienand, H. Saedler

   Pages 205-227

10. Direct Gene Transfer to Plants

    • I. Potrykus, J. Paszkowski, R. D. Shillito, M. W. Saul

    Pages 229-247

11. Microinjection: An Experimental Tool for Studying and Modifying Plant Cells

    • Brian L. A. Miki, Terry J. Reich, V. N. Iyer

    Pages 249-265

12. Transformation of Chlamydomonas Reinhardtii

    • J.-D. Rochaix

    Pages 267-284

13. Induction of Expression in and Stable Transformation of an Algal Cell by Nuclear Microinjection with Naked DNA

    • H.-G. Schweiger, G. Neuhaus

    Pages 285-302

14. Transient Expression of DNA in Plant Cells

    • Michael Fromm, Virginia Walbot

    Pages 303-310

15. Plastid Transformation: A Progress Report

    • M. J. Cornelissen, M. De Block, M. Van Montagu, J. Leemans, P. H. Schreier, J. Schell

    Pages 311-320

16. Targeting Nuclear Gene Products into Chloroplasts

    • Les J. Szabo, Anthony R. Cashmore

    Pages 321-339

17. Back Matter

    Pages 341-350

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There has been recent rapid progress in the transformation of plants with foreign DNA, making use either of the natural routes of genetic invasion that viruses and bacteria have developed, or of chemical, mechanical and electrical tricks to make plant protoplast membranes permeable to nucleic acids. Genes integrated into plant virus genomes can be carried systemi­ cally from the initial site of infection into the rest of the plant. Genes placed between the borders of Agrobacterium tumefaciens T-DNA can be transferred into single cells or plant tissue, which then divides to produce wound calli, or as in the case of an Agrobacterium rhizogenes infection, grow out into new roots. Calli and roots can be grown into whole plants. If virus genomes are placed between the T-DNA borders, a very effective infectious route, termed "agroinfection", is established. Once inside a pro­ toplast, DNA finds its way into the nucleus where it can finally integrate into the resident chromosome and be expressed. Whether it can also find its way into chloroplasts is not yet clear, but at least translation products can be targeted into this organelle. Regeneration of whole organisms from single cells is a special feature of plants and offers a unique tool to study genes in a multicellular organism. In addition, as in animal cells, transcription and translation of trans­ forming genes can be studied in plant cells during "transient expression".

• DNA
• plant

• Friedrich Miescher-Institut, Basel, Switzerland

  Thomas Hohn

• Max-Planch-Institut für Züchtungsforschung, Köln, Germany

  Joszef Schell

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