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Hydrocarbon and Lipid Microbiology Protocols

Synthetic and Systems Biology - Applications

  • Book
  • © 2016

Overview

Editors:
  1. Terry J. McGenity

    1. Dept. Biological Sciences, University of Essex, Colchester, United Kingdom

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    You can also search for this editor in PubMed   Google Scholar

  2. Kenneth N. Timmis

    1. Braunschweig, Germany

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    You can also search for this editor in PubMed   Google Scholar

  3. Balbina Nogales Fernández

    1. Dept. Biologia, Univ de les Illes Balears, Palma de Mallorca, Spain

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    You can also search for this editor in PubMed   Google Scholar

  • Offers readily-reproducible step-by-step laboratory methods
  • Provides helpful tips and tricks supporting the protocols
  • Includes troubleshooting advice
  • Contains practical laboratory guidance to promote successful results

Part of the book series: Springer Protocols Handbooks (SPH)

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Table of contents (8 protocols)

  1. Front Matter

    Pages i-x
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  2. Introduction to Systems and Synthetic Biology in Hydrocarbon Microbiology: Applications

    • Víctor de Lorenzo
    Pages 1-8

  3. Designing Bacteria to Produce Esters

    • Yi-Shu Tai, Kechun Zhang
    Pages 9-21

  4. Protocols for the Production and Analysis of Isoprenoids in Bacteria and Yeast

    • Claudia E. Vickers, Mareike Bongers, Sarah F. Bydder, Panagiotis Chrysanthopoulos, Mark P. Hodson
    Pages 23-52

  5. Synthetic Biology of Hydrophobic Polymer Production

    • Si Jae Park, Jae Ho Shin, Jung Eun Yang, Sang Yup Lee
    Pages 53-63

  6. Methods for Recombinant Rhamnolipid Production

    • Till Tiso, Andrea Germer, Benjamin Küpper, Rolf Wichmann, Lars M. Blank
    Pages 65-94

  7. Purification of Peptide Antimicrobials and Thioether-Stabilized Molecules Produced In Vivo by Lantibiotic Modification Machineries

    • Manuel Montalban-Lopez, Andrius Buivydas, Oscar P. Kuipers
    Pages 95-115

  8. Engineering Transcription Factor-Based Biosensors for the Detection of Intracellular Products

    • Solvej Siedler
    Pages 117-124

  9. Oxyfunctionalization of Linear Alkanes with a Biosynthetic, Self-Sufficient, Selective, and Soluble Hydroxylase

    • Mélanie Bordeaux, Jullien Drone
    Pages 125-137
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Keywords

About this book

This Volume presents protocols for systems and synthetic biology applications in the field of hydrocarbon and lipid microbiology. It complements another Volume that describes generic protocols for wet experimental and computer-based systems and synthetic biology studies. The protocols in this Volume demonstrate how to employ systems and synthetic biology approaches in the design of microbes for the production of esters, isoprenoids, hydrophobic polymers, rhamnolipid biosurfactant, and peptide antimicrobial and thioether-stabilised molecules. Also presented is a protocol for the engineering of transcription factor-based biosensors for intracellular products, and another for the creation of a synthetic hydroxylase with novel activity for the selective oxyfunctionalisation of linear alkanes.



Hydrocarbon and Lipid Microbiology Protocols
There are tens of thousands of structurally different hydrocarbons, hydrocarbon derivativesand lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes.













Editors and Affiliations

  • Dept. Biological Sciences, University of Essex, Colchester, United Kingdom

    Terry J. McGenity

  • Braunschweig, Germany

    Kenneth N. Timmis

  • Dept. Biologia, Univ de les Illes Balears, Palma de Mallorca, Spain

    Balbina Nogales Fernández

Bibliographic Information

  • Book Title: Hydrocarbon and Lipid Microbiology Protocols

  • Book Subtitle: Synthetic and Systems Biology - Applications

  • Editors: Terry J. McGenity, Kenneth N. Timmis, Balbina Nogales Fernández

  • Series Title: Springer Protocols Handbooks

  • DOI: https://doi.org/10.1007/978-3-662-49127-0

  • Publisher: Springer Berlin, Heidelberg

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer-Verlag Berlin Heidelberg 2016

  • Hardcover ISBN: 978-3-662-49126-3Published: 19 August 2016

  • Softcover ISBN: 978-3-662-56981-8Published: 15 June 2018

  • eBook ISBN: 978-3-662-49127-0Published: 09 August 2016

  • Series ISSN: 1949-2448

  • Series E-ISSN: 1949-2456

  • Edition Number: 1

  • Number of Pages: X, 137

  • Number of Illustrations: 20 b/w illustrations, 20 illustrations in colour

  • Topics: Microbiology, Biochemical Engineering

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