The Scid Mouse

1. Front Matter

   Pages I-XII

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2. General Phenotype/Cellular Characterization

   1. Front Matter

      Pages 1-1

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   2. The scid Mutation: Occurrence and Effect

      • Melvin J. Bosma

      Pages 3-9

   3. Pleiotropic Effects of the scid Mutation: Effects on Lymphoid Differentiation and on Repair of Radiation Damage

      • R. A. Phillips, G. M. Fulop

      Pages 11-17

   4. Analysis of Lymphoid Population in Scid Mice; Detection of a Potential B Lymphocyte Progenitor Population Present at Normal Levels in Scid Mice by Three Color Flow Cytometry With B220 and S7

      • R. R. Hardy, J. D. Kemp, K. Hayakawa

      Pages 19-25

   5. Phenotype and Differentiation Stage of Scid Mouse Thymocytes

      • Sonoko Habu, Yoko Norihisa, Takehito Sato, Hideo Yagita, Ko Okumura

      Pages 27-32

   6. Growth Requirements of B Lineage Lymphocytes From Scid and Normal Mice

      • G. Lee, K. Medina, P. W. Kincade

      Pages 33-37

   7. Defect of Scid Mouse Revealed in In Vitro Culture Systems

      • Shin-Ichi Nishikawa, Shin-Ichi Hayashi, Satomi Nishikawa, Minetaro Ogawa, Takahiro Kunisada, Tetsuo Sudo et al.

      Pages 39-46

   8. Natural Killer Cells and Their Precursors in Mice With Severe Combined Immunodeficiency

      • Vinay Kumar, J. Hackett Jr., M. M. Tutt, B. A. Garni-Wagner, W. A. Kuziel, P. W. Tucker et al.

      Pages 47-52

3. General Phenotype/Molecular Characterization

   1. Front Matter

      Pages 53-53

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   2. Nature of the scid Defect: A Defective VDJ Recombinase System

      • W. Schuler, M. J. Bosma

      Pages 55-62

   3. Rearrangement of T Cell Receptor Delta Genes in Thymus of Scid Mice

      • Ann M. Carroll, Melvin J. Bosma

      Pages 63-67

   4. Abnormal V(D)J Recombination in Murine Severe Combined Immune Deficiency: Absence of Coding Joints and Formation of Alternative Products

      • Michael R. Lieber, Joanne E. Hesse, Susanna Lewis, Gayle C. Bosma, Naomi Rosenberg, Kiyoshi Mizuuchi et al.

      Pages 69-75

   5. The scid Mutation Disrupts Gene Rearrangement at the Rejoining of Coding Strands

      • David Weaver, Eric Hendrickson

      Pages 77-84

   6. The Effect of the scid Mutation on Mechanism and Control of Immunoglobulin Heavy and Light Chain Gene Rearrangement

      • T. Keith Blackwell, Pierre Ferrier, Barbara A. Malynn, Roberta R. Pollock, Lori R. Covey, Heikyung Suh et al.

      Pages 85-94

4. Introduction of Functional Ig or TCR Transgenes

   1. Front Matter

      Pages 95-95

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   2. The Utilization of the scid Mutation in the Study of T Cell Development

      • Harald von Boehmer, Horst Blüthmann, Hung Sia Teh, Bernadette Scott

      Pages 97-105

   3. Transgenic Scid Mice With a Functionally Rearranged Immunoglobulin Heavy Chain Gene

      • Michal Fried, Richard R. Hardy, Melvin J. Bosma

      Pages 107-114

5. Leaky Phenotypes

   1. Front Matter

      Pages 115-115

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   2. T Cell Leakiness in Scid Mice

      • Ann M. Carroll, Richard R. Hardy, John Petrini, Melvin J. Bosma

      Pages 117-123

During routine genetic screening of several immunoglobulin heavy chain congenic mouse strains in 1980, one of us (MB) was surprised to find that several mice in the C.B-17IIcr strain, which was being maintained in a specific-pathogen-free facility of the Fox Chase Cancer Center (Philadelphia, PA), did not express serum immunoglobulin of the appropriate allotype. Fearing an error in the breeding of these mice, the sera of the suspect mice were screened for other allotypes. When these tests revealed a complete absence of serum immunoglobulin, it became apparent that a mutation had probably occurred in the C.B-17IIcr line. Further analysis revealed that a single breeding pair was respon­ sible for all of the immunoglobulin negative mice and that the defect showed recessive inheritance. Thus was the C.B-17/Icr scid or severe combined immune deficient (scid) mouse discovered. Although it has taken most animal facilities several years to breed scid mice of high quality for experimental purpose, it was clear by 1987 that many investigators were beginning to exploit the unique qualities of the scid mouse for studies in several areas.

• AIDS
• Biomedizinische Technik
• HIV
• Immunschwäche
• Leukämie
• Thymus
• bone
• immunobiology
• tissue
• transplantation

• Fox Chase Cancer Center, Institute for Cancer Research, Philadelphia, USA

  Melvin J. Bosma

• Division of Hematology/Oncology, Hospital for Sick Children, Departments of Immunology and Genetics, University of Toronto, Toronto, Canada

  Robert A. Phillips

• Basel Institute for Immunology, Basel, Switzerland

  Walter Schuler

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