Concepts in Biochemical Pharmacology

1. Front Matter

   Pages II-XX

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2. Methods of Studying the Metabolism of Drugs

   1. Assay of Drugs and Their Metabolites

      1. Basic Principles in Development of Methods for Drug Assay

         • Bernard B. Brodie

         Pages 1-8

      2. Absorption Spectrophotometry

         • R. P. Maickel, T. R. Bosin

         Pages 9-20

      3. Fluorometry

         • Helen S. Ackerman, Sidney Udenfriend

         Pages 21-41

      4. Radioactive Techniques: The Use of Labeled Drugs

         • R. P. Maickel, W. R. Snodgrass, R. Kuntzman

         Pages 42-57

      5. Radioactive Techniques: Radioactive Isotope Derivatives of Nonlabeled Drugs

         • R. Kuntzman, R. H. Cox Jr., R. P. Maickel

         Pages 58-62

      6. Gas Chromatography

         • M. W. Anders

         Pages 63-93

      7. Enzymatic Assays in Pharmacology

         • A. K. Cho

         Pages 94-107

      8. Bioassay

         • Marthe Vogt

         Pages 108-117

      9. Immunoassay

         • S. Spector

         Pages 118-122

   2. Isolation and Identification of Drug Metabolites

      1. Paper, Column and Thin Layer Chromatography, Counter-Current Distribution and Electrophoresis

         • Elwood O. Titus

         Pages 123-159

      2. Isotope Dilution Analysis

         • Vincent T. Oliverio, Anthony M. Guarino

         Pages 160-177

      3. Gas Chromatography-Mass Spectrometry

         • A. M. Guarino, H. M. Fales

         Pages 178-208

      4. The Application of Various Spectroscopies to the Identification of Drug Metabolites

         • Peter Bommer, Floie M. Vane

         Pages 209-225

3. Sites of Drug Metabolism

   1. Introduction: Pathways of Drug Metabolism

      • R. T. Williams

      Pages 226-242

   2. Microsomal Enzymes

      1. Some Morphological Characteristics of Hepatocyte Endoplasmic Reticulum and Some Relationships between Endoplasmic Reticulum, Microsomes, and Drug Metabolism

         • J. R. Fouts

         Pages 243-250

      2. Model Systems in Studies of the Chemistry and the Enzymatic Activation of Oxygen

         • V. Ullrich, Hj. Staudinger

         Pages 251-263

      3. Cytochrome P-450 — Its Function in the Oxidative Metabolism of Drugs

         • Ronald W. Estabrook

         Pages 264-284

      4. Enzymatic Oxidation at Carbon

         • J. Daly

         Pages 285-311

      5. <i>N</i>-Oxidation Enzymes

         • John H. Weisburger, Elizabeth K. Weisburger

         Pages 312-333

This volume of the Handbook of Experimental Pharmacology (Concepts in Biochemical Pharmacology) will show that pharma­ cology has finally arrived as a true discipline in its own right, and is no longer the handmaiden of organic chemistry and physiology. Instead it is an amalgam of all the biological sciences including biochemistry, biophysical chemistry, physiology, pathology and clinical medicine. In the volumes that make up Concepts in Bio­ chemical Pharmacology we hope to convince Medical Schools what should now be obvious, that pharmacology is no longer that dull topic bridging the basic sciences with medicine, but is probably the most important subject in the medical curriculum. We are grateful for the advice of Dr. Byron Clark, Director of the Pharmacology-Toxicology Program at the National Institutes of Health whose support made possible much of the work described in this volume. Contents Section Four: Methods 01 Stooging the MetoholiBm 01 Drugs Subsection A. Assay 01 Drugs and Their M etoholites Chapter 22 : Basic Principles in Development of Methods for Drug Assay. B. B. BRODIE. With 2 Figures 1 1 A. Introduction . . . . . . . . . . B. Principles of Developing a Method. . . . . . 1 I. Section of Method of Assay . . . . . . 1 II. Choice of Solvent for Extraction of Drug 2 III. Adsorption of Drugs by Glass Surfaces . . . . . . . . . . . . . . 3 IV. Recoveries of Known Amounts of Compound from Biological Material. 4 V. Assessment of Sensitivity 5 VI. Assessment of Specificity 5 References. . . . . . . . . . .

• assessment
• biochemistry
• chemistry
• drug
• medicine
• organic chemistry
• pathology
• pharmacology
• physiology
• research
• toxicology

• Department of Health, Education and Welfare, National Institutes of Health, Bethesda, USA

  Bernard B. Brodie, James R. Gillette

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