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In Living Color

Protocols in Flow Cytometry and Cell Sorting

  • Book
  • © 2000

Overview

Editors:
  1. Rochelle A. Diamond

    1. Division of Biology, California Institute of Technology, Pasadena, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. Susan Demaggio

    1. Optical Biology Core Facility Developmental Biology Center, University of California, Irvine, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Flow cytometry and cell sorting are becoming increasingly important in biological research
  • Step-by-step approach to the methodology for measuring various cellular attributes
  • Includes supplementary material: sn.pub/extras

Part of the book series: Springer Lab Manuals (SLM)

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Table of contents (57 chapters)

  1. Front Matter

    Pages I-XXV
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  2. Section 1

    1. Front Matter

      Pages 1-1
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    2. A Palette of Living Colors

      • Rochelle Diamond
      Pages 3-7

    3. The Colorful History of Flow Cytometry

      • Susan Demaggio
      Pages 9-10

  3. Section 2

    1. Front Matter

      Pages 11-11
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    2. Getting Started

      • Rochelle Diamond
      Pages 13-13

    3. How the FACSCalibur and FACS Vantage Work and Why It Matters

      • Tom Frey
      Pages 15-38

    4. How Flow Cytometers Work — and Don’t Work

      • Howard Shapiro
      Pages 39-56

    5. Flow Cytometry Standard (FCS) Data File Format

      • Larry Seamer
      Pages 57-61

    6. Options for Data Display

      • Susan Demaggio
      Pages 62-70

    7. Guidelines to Improve Flow Cytometry Data Display and Interpretation

      • Wendy Schober-Ditmore
      Pages 71-75

    8. Data Management and Storage

      • David M. Coder
      Pages 76-91

    9. Coloring Up: A Guide to Spectral Compensation

      • Rochelle A. Diamond
      Pages 92-97

    10. Quality Control Guidelines for Research Flow Cytometry

      • Rochelle A. Diamond
      Pages 98-105

  4. Section 3

    1. Front Matter

      Pages 107-107
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    2. Sample Preparation and Cell Surface Staining

      • Rochelle A. Diamond
      Pages 109-109

    3. Cell Preparation and Enrichment for FCM Analysis and Cell Sorting

      • Rochelle A. Diamond, Hua Wang, Fei Chen, Mindy Wilke-Douglas
      Pages 111-141

    4. The Basics of Staining for Cell Surface Proteins

      • Alice L. Givan
      Pages 142-164

    5. Phenotypic Analysis Using Five-Colour Immunofluorescence and Flow Cytometry

      • Andy Beavis
      Pages 165-174

    6. Detection of Cell Surface Antigens Using Antibody-conjugated Fluorospheres and Flow Cytometry

      • Andy Beavis
      Pages 175-183
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Keywords

About this book

Advances in the field of cell biology have always been closely related to the development of quantitative analytical methods that can be applied to individual cells or cell organelles. Almost from the early stages following the invention of the microscope, the investigator has been keenly interested in obtaining informa­ tion on the functionality of single cells and how cells perform under different sets of experimental conditions. Although cells could be viewed in the microscope for a few hundred years, only since the relatively recent application of autoradiography did we come to realize that, although cells may visually appear very much alike, they are quite different in their functional capacity. The quest to understand these differences in a cell population lead to a new series of techniques for labeling and quantitating DNA content and similar approaches have driven the develop­ ment of methods for analyzing various other cellular properties. The development of new analytical techniques follows the age old pattern of applying successes of the past with current inno­ vation, logic and new biological information. Results from auto­ radiography expanded the concept of the cell cycle from inter­ phase and mitosis to the more definitive GO/GI, Sand G2/M phases. This new knowledge lead to the development of techno­ logy to measure and analyze various parameters related to the cell cycle.

Editors and Affiliations

  • Division of Biology, California Institute of Technology, Pasadena, USA

    Rochelle A. Diamond

  • Optical Biology Core Facility Developmental Biology Center, University of California, Irvine, USA

    Susan Demaggio

Bibliographic Information

  • Book Title: In Living Color

  • Book Subtitle: Protocols in Flow Cytometry and Cell Sorting

  • Editors: Rochelle A. Diamond, Susan Demaggio

  • Series Title: Springer Lab Manuals

  • DOI: https://doi.org/10.1007/978-3-642-57049-0

  • Publisher: Springer Berlin, Heidelberg

  • eBook Packages: Springer Book Archive

  • Copyright Information: Springer-Verlag Berlin Heidelberg 2000

  • Hardcover ISBN: 978-3-540-65149-9Due: 06 March 2000

  • Softcover ISBN: 978-3-642-62978-5Published: 05 October 2013

  • eBook ISBN: 978-3-642-57049-0Published: 06 December 2012

  • Edition Number: 1

  • Number of Pages: XXV, 800

  • Number of Illustrations: 158 b/w illustrations, 13 illustrations in colour

  • Topics: Cell Biology, Molecular Medicine, Biochemistry, general

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