Steroid-Protein Interactions

1. Front Matter

   Pages I-XIII

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2. Introduction

   • Ulrich Westphal

   Pages 1-3

3. Early Studies on Interactions between Proteins and Small Molecules or Ions

   • Ulrich Westphal

   Pages 4-10

4. First Observations on Reversible Binding of Steroids to Proteins

   • Ulrich Westphal

   Pages 11-14

5. Basic Methods for Determination of Steroid-Protein Interactions

   • Ulrich Westphal

   Pages 15-52

6. Characteristics of Steroid-Protein Interactions

   • Ulrich Westphal

   Pages 53-83

7. Steroid Interactions with Serum Proteins Other than Specific Glycoproteins

   • Ulrich Westphal

   Pages 84-132

8. Influence of Molecular Structure on Steroid Interaction with HSA and Other Proteins

   • Ulrich Westphal

   Pages 133-163

9. Corticosteroid-Binding Globulin (CBG) of Man and Other Species

   • Ulrich Westphal

   Pages 164-236

10. Influence of Various Endogenous Factors on CBG Activity in the Rat

    • Ulrich Westphal

    Pages 237-293

11. CBG Activity in Mouse, Rabbit and Guinea Pig during Pregnancy and Lactation; PBG in the Guinea Pig

    • Ulrich Westphal

    Pages 294-314

12. Isolation and Properties of CBG from Mammalian Sera

    • Ulrich Westphal

    Pages 315-355

13. High-Affinity Binding of Estradiol and Testosterone to Serum Proteins

    • Ulrich Westphal

    Pages 356-374

14. Interaction of Progesterone and Other Steroids with α₁-Acid Glycoprotein

    • Ulrich Westphal

    Pages 375-433

15. Effect of Protein Binding on Hormonal Activity of Steroids

    • Ulrich Westphal

    Pages 434-445

16. Steroid-Binding Immunoglobulins: Preparation, Properties and Application for Radioimmunoassay of Steroid Hormones

    • Ulrich Westphal

    Pages 446-462

17. Notes on Steroid Interactions with Enzymes

    • Ulrich Westphal

    Pages 463-476

18. Synopsis

    • Ulrich Westphal

    Pages 477-480

19. Back Matter

    Pages 481-568

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This is the story of steroid-protein interactions as one investigator sees it. Following the general concept of this monograph series, it emphasizes the results and interpretations obtained in the author's laboratory, and is therefore a subjective account. Recognizing, how­ ever, that the discussion of the subject would be incomplete if the material were limited to one scientist's work, the essential achieve­ ments of other investigators have been incorporated. An effort has thus been made to give a balanced presentation and to enable the reader to see in perspective the varied facets of the interactions between steroids and proteins. Since this is the first comprehensive treatment of the topic, it seems appropriate to go to the roots, and try to find out how it all started. The first chapters, therefore, take the reader to the laboratories of those who very early conceived the significance of the attachment of dyes, drugs, and other conspicuous molecules to those colloids called proteins. The discovery of the steroid hormones set the stage for meaningful investigation of their interaction with proteins of various origins and functions - a process which is continuing today with increasing vigor.

• hormone
• protein
• proteins
• steroid
• steroids

• School of Medicine, University of Louisville, Louisville, USA

  Ulrich Westphal

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