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Guidelines for Molecular Analysis in Archive Tissues

  • Book
  • © 2011

Overview

Editors:
  1. Giorgio Stanta

    1. c/o Ospedale di Cattinara, University of Trieste, Trieste, Italy

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  • Provides detailed guidelines for molecular analysis of archive tissues

  • An invaluable aid for researchers and pathologists involved in translational research and diagnostics

  • Caters for the needs of the less experienced through the inclusion of clear notes and explanations

  • Written by experts who have developed the described methods and validated them within the framework of a multi-institution European project

  • Includes supplementary material: sn.pub/extras

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Table of contents (52 chapters)

  1. Front Matter

    Pages i-xvi
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  2. Archive Tissues

    1. Front Matter

      Pages 1-1
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    2. Archive Tissues

      • Giorgio Stanta
      Pages 3-4

    3. Preanalytical Time Interval (PATI) and Fixation

      • Lorenzo Daniele, Giuseppe D’Armento, Gianni Bussolati
      Pages 5-11

    4. Formalin-Free Fixatives

      • Isabella Dotti, Serena Bonin, Giorgio Basili, Valentina Faoro
      Pages 13-18

  3. Dissection of Tissue Components

    1. Front Matter

      Pages 19-19
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    2. Manual Microdissection

      • Valentina Faoro, Giorgio Stanta
      Pages 21-22

    3. Tissue Microarray (TMA)

      • Valentina Faoro, Anna Sapino
      Pages 23-26

    4. Laser Capture Microdissection (LCM)

      • Elvira Stacher, Hannelore Kothmaier, Iris Halbwedl, Helmut H. Popper
      Pages 27-29

  4. Extraction, Purification, Quantification and Quality Assessment of Nucleic Acids

    1. Front Matter

      Pages 31-31
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    2. DNA Extraction from Formalin-Fixed Paraffin-Embedded (FFPE) Tissues

      • Serena Bonin, Patricia J. T. A. Groenen, Iris Halbwedl, Helmut H. Popper
      Pages 33-36

    3. DNA Extraction from Formalin-Fixed Paraffin-Embedded Tissues (FFPE) (from Small Fragments of Tissues or Microdissected Cells)

      • Serena Bonin, Patricia J. T. A. Groenen, Iris Halbwedl, Helmut H. Popper
      Pages 37-39

    4. Fast Protocol for DNA Extraction from Formalin-Fixed Paraffin-Embedded Tissues

      • Falk Hlubek, Andreas Jung
      Pages 41-43

    5. DNA Extraction from Blood and Forensic Samples

      • Solange Sorçaburu Cigliero, Elisabetta Edalucci, Paolo Fattorini
      Pages 45-54

    6. Restoration and Reconstruction of DNA Length

      • Serena Bonin, Federica Tavano
      Pages 55-56

    7. RNA Extraction from Formalin-Fixed Paraffin-Embedded Tissues

      • Serena Bonin, Giorgio Stanta
      Pages 57-61

    8. RNA Extraction from Decalcified and Non-decalcified Formalin-Fixed Paraffin-Embedded Tissues

      • Marco Alberghini, Stefania Benini, Gabriella Gamberi, Stefania Cocchi, Licciana Zanella
      Pages 63-66

    9. RNA Temperature Demodification

      • Serena Bonin, Giorgio Stanta
      Pages 67-69

    10. MicroRNA Extraction from Formalin-Fixed Paraffin-Embedded Tissues

      • Roberto Cirombella, Andrea Vecchione
      Pages 71-74

    11. Quantification of Nucleic Acids

      • Isabella Dotti, Serena Bonin
      Pages 75-79
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Keywords

About this book

A huge amount of fixed and paraffin-embedded tissue is stored in every hospital. This is very precious material that can be used for translational research and for diagnostics. The molecular methods employed for analysis of these tissues are similar to the usual molecular biology and proteomics methods, but reliable results can be obtained only if specific steps are followed with great care. This book provides detailed and precise guidelines for molecular analysis of archive tissues and will serve as an invaluable aid for researchers and pathologists involved in translational research and diagnostics. Clear notes and explanations are included to simplify use of the protocols for the less experienced. The authors are a group of acknowledged experts who have developed the described methods and validated them within the European project "Archive Tissues: Improving Molecular Medicine Research and Clinical Practice - IMPACTS", which has involved 21 leading institutions in 11 countries.

Reviews

From the reviews:

“This is a valuable resource that provides precise guidelines for molecular analysis of archive tissues essential for pathologists and researchers involved in translational research and diagnostics. … students, laboratory technicians, and physician-scientists interested in molecular analysis of archive tissues will find this book useful. … The clear explanations should enable even less experienced researchers to follow the protocols.”­­­ (Omer Iqbal, Doody’s Review Service, December, 2011)

Editors and Affiliations

  • c/o Ospedale di Cattinara, University of Trieste, Trieste, Italy

    Giorgio Stanta

About the editor

Giorgio Stanta is Professor of Pathology at the University of Trieste. For many years his main interest has been the application of molecular analysis to fixed and paraffin-embedded tissues (all human tissues of biopsy or surgical origin), called “archive tissues”. This research line started thanks to his collaboration with the researchers who first applied molecular biology techniques to human diseases at Yale University, where Professor Stanta worked from 1985 to 1988. Since then he has developed several methods, in particular for RNA analysis. Professor Stanta is the coordinator of the European project "Archive Tissues: Improving Molecular Medicine Research and Clinical Practice - IMPACTS" (www.impactsnetwork.eu), involving around 20 European universities in 11 different countries. He is taking part as an expert in the Biobanking and Biomolecular Resources Research European Infrastructure (BBMRI). He is also on the Managing Board of the "Molecular Pathology Study Group" of the European Society of Pathology.

Bibliographic Information

  • Book Title: Guidelines for Molecular Analysis in Archive Tissues

  • Editors: Giorgio Stanta

  • DOI: https://doi.org/10.1007/978-3-642-17890-0

  • Publisher: Springer Berlin, Heidelberg

  • eBook Packages: Medicine, Medicine (R0)

  • Copyright Information: Springer-Verlag Berlin Heidelberg 2011

  • Hardcover ISBN: 978-3-642-17889-4Due: 27 June 2011

  • Softcover ISBN: 978-3-662-51984-4Published: 23 August 2016

  • eBook ISBN: 978-3-642-17890-0Published: 06 June 2011

  • Edition Number: 1

  • Number of Pages: XVI, 323

  • Topics: Pathology, Molecular Medicine, Oncology

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