Microscopy of the Heart

1. Front Matter

   Pages i-vii

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2. Studying Structure and Function of the Heart Cells Using Scanning Ion Conductance Microscopy

   • Anamika Bhargava, Julia Gorelik

   Pages 1-20

3. Optical Sectioning Microscopy at ‘Temporal Super-Resolution’

   • Katja Flügel, Qinghai Tian, Lars Kaestner

   Pages 21-35

4. Quantitative Super-Resolution Microscopy of Cardiomyocytes

   • Christian Soeller, Izzy D. Jayasinghe

   Pages 37-73

5. Caged Compounds: Applications in Cardiac Muscle Research

   • Ernst Niggli, Natalia Shirokova

   Pages 75-95

6. Optogenetic Tools in the Microscopy of Cardiac Excitation-Contraction Coupling

   • Lars Kaestner, André Zeug, Qinghai Tian

   Pages 97-117

7. Electron Microscopy

   • Sergio Bertazzo

   Pages 119-132

This book provides in depths information on different microscopy approaches and supplies the reader with methods how to untangle highly complex processes involved in physiological and pathophysiological cardiac signaling. Microscopy approaches have established themselves as the quasi gold standard that enables us to appreciate the underlying mechanisms of physiological and pathophysiological cardiac signaling. This book presents the most important microscopy techniques from the level of individual molecule e.g. Förster-Resonance Energy Transfer (FRET), up to cellular and tissue imaging, e.g. electron microscopy (TEM) or light sheet microscopy. The book is intended for graduate students and postdocs in cardiovascular research, imaging and cell biology, pre-clinical and clinical researchers in cardiovascular sciences as well as decision makers of the pharmaceutical industry.

• Atomic Force Microscopy
• Heart Disease
• Imaging
• Optical Imaging of the Heart
• Positron Emission Tomography
• Scanning Probe Imaging
• FRET
• MRI
• Magnetic Resonance Imaging
• Förster-Resonance Energy Transfer
• imaging of individual molecules
• whole body imaging
• pathophysiological cardiac signaling

• Theoretical Medicine and Biosciences, Saarland University, Homburg/Saar, Germany

  Lars Kaestner

• Preclinical Center for Molecular Signal Processing, Building 61, Saarland University, Homburg/Saar, Germany

  Peter Lipp

Lars Kaestner PhD, is principal investigator at Theoretical Medicine and Biosciences, Saarland University, Homburg, and Experimental Physics, Saarland University, Saarbrücken, Germany. He studies basic cellular mechanisms of the circulation (heart and red blood cells) using newest imaging methods



Peter Lipp is Professor and Head of the Institute for Molecular Cell Biology, Saarland University, in Homburg, Germany. The main focus of his institute lies on studying basic cellular mechanisms of the heart in its clinical context, with the aim to develop new therapies and treatment strategies.

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