Pancreatic Islet Isolation

1. Front Matter

   Pages i-xii

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2. Historical Background of Pancreatic Islet Isolation

   • Miriam Ramírez-Domínguez

   Pages 1-9

3. The Different Faces of the Pancreatic Islet

   • Midhat H. Abdulreda, Rayner Rodriguez-Diaz, Over Cabrera, Alejandro Caicedo, Per-Olof Berggren

   Pages 11-24

4. Isolation of Mouse Pancreatic Islets of Langerhans

   • Miriam Ramírez-Domínguez

   Pages 25-34

5. Pancreatic Islets: Methods for Isolation and Purification of Juvenile and Adult Pig Islets

   • Heide Brandhorst, Paul R. V. Johnson, Daniel Brandhorst

   Pages 35-55

6. Isolation of Pancreatic Islets from Nonhuman Primates

   • Dora M. Berman

   Pages 57-66

7. Necessities for a Clinical Islet Program

   • Wayne J. Hawthorne

   Pages 67-88

8. Clinical Islet Isolation

   • Wayne J. Hawthorne, Lindy Williams, Yi Vee Chew

   Pages 89-122

9. Back Matter

   Pages 123-123

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Pancreatic islets make up the endocrine pancreas and they contain the only source of insulin in the body, beta cells. Hence, access to high quality preparations of pancreatic islets is fundamental for in vitro studies and to test pre-clinical applications in animal models in vivo. Access to healthy human islets is also crucial to improve transplantation procedures for diabetes. Given the susceptibility of pancreatic islets to the enzymatic digestion and mechanical stress required to obtain them, the isolation of islets is often considered as the delicate “work of a craftsman”. This book, which is aimed at beginners and experts alike, is a survey of the current state-of–the-art in this field and it centres on the challenges, pitfalls and peculiarities of pancreatic islet isolation in the different species used in pre-clinical and clinical applications. It explores the similarities and differences between human islets and those from other relevantspecies (rodents, pigs and non-human primates), and how these influence islet isolation. The ultimate goal of this book is to improve the outcome of islet isolation and transplantation in pre-clinical and clinical applications.

• Animal Models
• Diabetes
• Human Islet Isolation
• Islet Purification
• Translational Studies

• Laboratory of Cell Therapy of Diabetes, Department of Pediatrics, Faculty of Medicine and Odontology, Hospital Cruces, University of the Basque Country (UPV/EHU), Leioa, Spain

  Miriam Ramírez-Domínguez

Miriam Ramírez-Domínguez, University of the Basque Country, Faculty of Medicine, Laboratory of Cell Therapy of Diabetes, Leioa, Spain

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