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Microfluidic Methods for Molecular Biology

  • Book
  • © 2016

Overview

Editors:
  1. Chang Lu

    1. Virginia Tech, Blacksburg, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. Scott S. Verbridge

    1. Dpt of Biomedical Engg. & Mechanics, Virginia Tech, Blacksburg, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Reviews exhaustively the latest techniques for single-cell genetic, epigenetic, metabolomic, and proteomic analysis
  • Illustrates microfluidic approaches for inverse metabolic engineering, as well as analysis of circulating exosomes
  • Broadens readers’ understanding of microfluidics convection-based PCR technology, microfluidic RNA-seq, and microfluidics for robust mobile diagnostics
  • Includes supplementary material: sn.pub/extras

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Table of contents (16 chapters)

  1. Front Matter

    Pages i-xii
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  2. Microfluidic Platforms for Quantitative Biology Studies in Model Organisms

    • Daniel A. Porto, Tel M. Rouse, Adriana San-Miguel, Hang Lu
    Pages 1-18

  3. Microfluidic Methods in Single Cell Biology

    • Arnab Mukherjee, Charles M. Schroeder
    Pages 19-54

  4. Convective PCR Thermocycling with Smartphone-Based Detection: A Versatile Platform for Rapid, Inexpensive, and Robust Mobile Diagnostics

    • Aashish Priye, Victor M. Ugaz
    Pages 55-69

  5. Forensic Typing of Single Cells Using Droplet Microfluidics

    • Tao Geng, Richard A. Mathies
    Pages 71-94

  6. Microfluidic Approaches to Fluorescence In Situ Hybridization (FISH) for Detecting RNA Targets in Single Cells

    • Robert J. Meagher, Meiye Wu
    Pages 95-112

  7. Microfluidic Multistage Integration for Analysis of Circulating Exosomes

    • Mei He, Andrew Godwin, Yong Zeng
    Pages 113-139

  8. Microfluidic Single-Cell Functional Proteomics

    • Shay Mailloux, Lisa Ramirez, Jun Wang
    Pages 141-159

  9. Microfluidics for DNA and Protein Analysis with Multiplex Microbead-Based Assays

    • Wanqing Yue, Mengsu Yang
    Pages 161-187

  10. Single-Cell Phenotypic Screening in Inverse Metabolic Engineering

    • A. E. Vasdekis, G. Stephanopoulos
    Pages 189-204

  11. Microfluidic Paper-Based Multiplexing Biosensors for Electrochemical Detection of Metabolic Biomarkers

    • Chen Zhao, Martin M. Thuo, Xinyu Liu
    Pages 205-218

  12. Droplet Microfluidics for Screening of Surface-Marker and Secretory Protein Expression

    • Pooja Sabhachandani, Saheli Sarkar, Tania Konry
    Pages 219-233

  13. Wire-guided Droplet Manipulation for Molecular Biology

    • Dustin K. Harshman, Jeong-Yeol Yoon
    Pages 235-252

  14. Mechanical and Electrical Principles for Separation of Rare Cells

    • Elisa M. Wasson, Temple A. Douglas, Rafael V. Davalos
    Pages 253-294

  15. Microfluidics for High-Throughput Cellular Isolation and Analysis in Biomedicine

    • Caroline N. Jones, Joseph M. Martel-Foley
    Pages 295-322

  16. Microfluidics for Cell Culture

    • Deepika Devadas, Edmond W. K. Young
    Pages 323-347

  17. Microfluidic Chromatin Immunoprecipitation for Analysis of Epigenomic Regulations

    • Yan Zhu, Chang Lu
    Pages 349-363

  18. Back Matter

    Pages 365-376
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Keywords

About this book

This book covers the state-of-the-art research on molecular biology assays and molecular techniques enabled or enhanced by microfluidic platforms. Topics covered include microfluidic methods for cellular separations and single cell studies, droplet-based approaches to study protein expression and forensics, and microfluidic in situ hybridization for RNA analysis. Key molecular biology studies using model organisms are reviewed in detail. This is an ideal book for students and researchers in the microfluidics and molecular biology fields as well as engineers working in the biotechnology industry.

This book also:



  • Reviews exhaustively the latest techniques for single-cell genetic, epigenetic, metabolomic, and proteomic analysis
  • Illustrates microfluidic approaches for inverse metabolic engineering, as well as analysis of circulating exosomes
  • Broadens readers’ understanding of microfluidics convection-based PCRtechnology, microfluidic RNA-seq, and microfluidics for robust mobile diagnostics

Editors and Affiliations

  • Virginia Tech, Blacksburg, USA

    Chang Lu

  • Dpt of Biomedical Engg. & Mechanics, Virginia Tech, Blacksburg, USA

    Scott S. Verbridge

About the editors

Chang Lu is a Professor of Chemical Engineering at Virginia Tech. His research focuses on developing microfluidic biotechnologies for cellular and molecular analysis with recent emphasis on low-input epigenomic studies. Scott Verbridge is an Assistant Professor in the Virginia Tech Department of Biomedical Engineering and Mechanics, and the Virginia Tech - Wake Forest School of Biomedical Engineering and Sciences. His research is focused on developing engineered tumor models to better understand cell-microenvironment interactions, and their impact on tumor progression and therapy response.  

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