Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 1130)
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Table of contents (27 protocols)
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Front Matter
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Understanding Mouse Skeletal Development and Repair
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Front Matter
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Skeletal Repair, Transplantations, and Organ Cultures
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Front Matter
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Histological and Molecular Analyses on Whole Tissues and Tissue Sections
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Front Matter
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Keywords
About this book
Skeletal Development and Repair: Methods and Protocols is a compilation of a variety of skeletal research protocols utilizing the laboratory mouse as the platform for surgical manipulation and/or transplantation as well as the source of tissues and cells for in vitro culture and analyses. Chapters are written by experts in the field and cover topics including surgical, transplantation and organ culture methods that permit analyses of skeletal tissues undergoing repair in vivo and permits analyses of cellular interactions ex vivo, histological and molecular techniques developed to study gene and protein expression in whole embryos, skeletal tissues and tissue sections and in vitro primary cell culture protocols designed to assay gene function in specific cell populations. Written in the successful Methods in Molecular Biology series format chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Skeletal Development and Repair: Methods and Protocols is a comprehensive laboratory manual for all levels of basic research scientists working in the broad fields of skeletal development and skeletal repair research.
Editors and Affiliations
Bibliographic Information
Book Title: Skeletal Development and Repair
Book Subtitle: Methods and Protocols
Editors: Matthew J. Hilton
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-62703-989-5
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2014
Hardcover ISBN: 978-1-62703-988-8Published: 31 January 2014
Softcover ISBN: 978-1-4939-6310-2Published: 23 August 2016
eBook ISBN: 978-1-62703-989-5Published: 30 January 2014
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: X, 328
Number of Illustrations: 38 b/w illustrations, 40 illustrations in colour
Topics: Molecular Medicine, Regenerative Medicine/Tissue Engineering