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  • © 2014

Plant-Pathogen Interactions

Methods and Protocols

  • Presents current up-to-date methodologies
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1127)

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Table of contents (22 protocols)

  1. Front Matter

    Pages i-xiii
  2. Bioinformatics

    1. Front Matter

      Pages 1-1
    2. Galaxy as a Platform for Identifying Candidate Pathogen Effectors

      • Peter J. A. Cock, Leighton Pritchard
      Pages 3-15
    3. On the Statistics of Identifying Candidate Pathogen Effectors

      • Leighton Pritchard, David Broadhurst
      Pages 53-64
  3. Cell Biology

    1. Front Matter

      Pages 65-65
    2. High-Throughput Imaging of Plant Immune Responses

      • Martina Beck, Ji Zhou, Christine Faulkner, Silke Robatzek
      Pages 67-80
    3. In Vivo Protein–Protein Interaction Studies with BiFC: Conditions, Cautions, and Caveats

      • Petra Boevink, Hazel McLellan, Tatyana Bukharova, Stefan Engelhardt, Paul Birch
      Pages 81-90
  4. From Assays of Pathogen Virulence to Effector Function

    1. Front Matter

      Pages 111-111
    2. Functional Characterization of Nematode Effectors in Plants

      • Axel A. Elling, John T. Jones
      Pages 113-124
    3. Silencing of Aphid Genes by Feeding on Stable Transgenic Arabidopsis thaliana

      • Alexander D. Coleman, Marco Pitino, Saskia A. Hogenhout
      Pages 125-136
    4. A Growth Quantification Assay for Hyaloperonospora arabidopsidis Isolates in Arabidopsis thaliana

      • Daniel F. A. Tomé, Jens Steinbrenner, Jim L. Beynon
      Pages 145-158
    5. Simple Quantification of In Planta Fungal Biomass

      • Michael Ayliffe, Sambasivam K. Periyannan, Angela Feechan, Ian Dry, Ulrike Schumann, Evans Lagudah et al.
      Pages 159-172
    6. A Simple and Fast Protocol for the Protein Complex Immunoprecipitation (Co-IP) of Effector: Host Protein Complexes

      • Jens Steinbrenner, Matthew Eldridge, Daniel F. A. Tomé, Jim L. Beynon
      Pages 195-211

About this book

Plant-Pathogen Interactions: Methods and Protocols, Second Edition expands upon the first edition with current, detailed protocols for the study of plant pathogen genome sequences. It contains new chapters on techniques to help identify and characterize effectors and to study their impacts on host immunity and their roles in pathogen biology. Additional chapters focus on protocols to identify avirulence and resistance genes, investigate the roles of effector targets and other defence-associated proteins in plant immunity. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Plant-Pathogen Interactions: Methods and Protocols, Second Edition seeks to aid scientists in the further study of plant immunity.

Editors and Affiliations

  • Dundee Effector Consortium, Cell and Molecular Sciences, The James Hutton Institute, Dundee, United Kingdom

    Paul Birch

  • Cell and Molecular Sciences,The James Hutton Institute, Dundee, United Kingdom

    John T. Jones

  • Cell and Molecular Sciences, The James Hutton Institute, Dundee, United Kingdom

    Jorunn I.B. Bos

Bibliographic Information

  • Book Title: Plant-Pathogen Interactions

  • Book Subtitle: Methods and Protocols

  • Editors: Paul Birch, John T. Jones, Jorunn I.B. Bos

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-986-4

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, New York 2014

  • Hardcover ISBN: 978-1-62703-985-7Published: 19 March 2014

  • Softcover ISBN: 978-1-4939-6323-2Published: 24 September 2016

  • eBook ISBN: 978-1-62703-986-4Published: 18 March 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XIII, 306

  • Number of Illustrations: 14 b/w illustrations, 43 illustrations in colour

  • Topics: Plant Sciences, Plant Genetics and Genomics

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access