Skip to main content
  • Book
  • © 2014

The Complement System

Methods and Protocols

Editors:

  • Presents current up-to-date methodologies
  • Features tips on trouble shooting and avoiding known pitfalls
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1100)

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

This is a preview of subscription content, log in via an institution to check for access.

Table of contents (32 protocols)

  1. Front Matter

    Pages i-xvi
  2. Overview

    • Mihaela Gadjeva
    Pages 1-9
  3. Evaluation of Complement Function by ELISA

    • Anja Roos, Jörgen Wieslander
    Pages 11-23
  4. Classical Complement Pathway Components C1r and C1s: Purification from Human Serum and in Recombinant Form and Functional Characterization

    • Véronique Rossi, Isabelle Bally, Monique Lacroix, Gérard J. Arlaud, Nicole M. Thielens
    Pages 43-60
  5. Expression and Purification Methods for the Production of Recombinant Human Complement Component C2

    • Angela W. Norton, Paolo G. V. Martini, Lynette C. Cook, Scott Alderucci, Dianna M. Lundberg, Susan M. Fish et al.
    Pages 61-74
  6. Purification and Characterization of Human and Mouse Complement C3

    • Marieta M. Ruseva, Meike Heurich
    Pages 75-91
  7. Purification of Human Complement Protein C5

    • Lars Sottrup-Jensen, Gregers Rom Andersen
    Pages 93-102
  8. Quantification of Complement C5b-9 Binding to Cells by Flow Cytometry

    • Oren Moskovich, Zvi Fishelson
    Pages 103-108
  9. Genotyping of FCN and MBL2 Polymorphisms Using Pyrosequencing

    • Lea Munthe-Fog, Hans Ole Madsen, Peter Garred
    Pages 123-130
  10. Purification, Measurement of Concentration, and Functional Complement Assay of Human Ficolins

    • Misao Matsushita, David Kilpatrick, Hiroshi Shiraki, Yu Liu, Koichiro Tateishi, Mitsushi Tsujimura et al.
    Pages 141-159
  11. Purification and Functional Analysis of Human Properdin

    • Joseph O’Flynn, Maria C. Faber Krol, Mohamed R. Daha, Cees van Kooten
    Pages 161-167
  12. Purification and Functional Characterization of Factor I

    • Sara C. Nilsson, Anna M. Blom
    Pages 177-188
  13. C1 Inhibitor: Quantification and Purification

    • Lilian Varga, József Dobó
    Pages 189-205
  14. Purification, Quantification, and Functional Analysis of Complement Factor H

    • Bing-Bin Yu, Beryl E. Moffatt, Marina Fedorova, Claire G. S. Villiers, James N. Arnold, Eugenie Du et al.
    Pages 207-223
  15. Factor H-Related Proteins

    • Mihály Józsi, Seppo Meri
    Pages 225-236
  16. Functional Evaluation of Factor H genetic and Acquired Abnormalities: Application for Atypical Hemolytic Uremic Syndrome (aHUS)

    • Lubka T. Roumenina, Roxane Roquigny, Caroline Blanc, Nelly Poulain, Stéphanie Ngo, Marie-Agnès Dragon-Durey et al.
    Pages 237-247

About this book

Complement Systems: Methods and Protocols is composed of 32 individual chapters that describe a variety of protocols  to purify and analyze the activity of the individual complement components or pathways.  It includes assays that describe detection  of complement SNPs,  clinical methods to evaluate complement system activation and data interpretation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Complement Systems: Methods and Protocols provides a collection of well-established “classical” assays and recently developed “new” assays to analyze the complement system activation will be useful to a wide audience of scientists.

Editors and Affiliations

  • Division of Infectious Diseases, Brigham and Women’s Hospital, Harvard University Harvard Medical School, Boston, USA

    Mihaela Gadjeva

Bibliographic Information

  • Book Title: The Complement System

  • Book Subtitle: Methods and Protocols

  • Editors: Mihaela Gadjeva

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-724-2

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, New York 2014

  • Hardcover ISBN: 978-1-62703-723-5Published: 12 November 2013

  • Softcover ISBN: 978-1-4939-6306-5Published: 23 August 2016

  • eBook ISBN: 978-1-62703-724-2Published: 11 November 2013

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVI, 383

  • Number of Illustrations: 54 b/w illustrations, 27 illustrations in colour

  • Topics: Immunology, Antibodies

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access