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Epiblast Stem Cells

Methods and Protocols

  • Book
  • © 2013

Overview

Editors:
  1. Ramiro Alberio

    1. School of Biosciences Rm 223 South Lab, University of Nottingham, Loughborough, United Kingdom

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  • Serves as a resource to individuals interested in studying the biology of pluripotent cells
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1074)

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Table of contents (15 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Generation and Characterization of Epiblast Stem Cells from Blastocyst-Stage Mouse Embryos

    • Daniel C. Factor, Fadi J. Najm, Paul J. Tesar
    Pages 1-13

  3. Reversion of Mouse Postimplantation Epiblast Stem Cells to a Naïve Pluripotent State by Modulation of Signalling Pathways

    • Astrid Gillich, Siqin Bao, M. Azim Surani
    Pages 15-29

  4. Converting Mouse Epiblast Stem Cells into Mouse Embryonic Stem Cells by Using Small Molecules

    • Wenlin Li, Sheng Ding
    Pages 31-37

  5. Isolation and Culture of Rabbit Embryonic Stem Cells

    • Arata Honda
    Pages 39-49

  6. Goat Embryonic Stem-Like Cell Derivation and Characterization

    • Esmail Behboodi, Louise Lam, William G. Gavin, Alla Bondareva, Ina Dobrinski
    Pages 51-67

  7. Derivation and Culture of Canine Embryonic Stem Cells

    • Ian C. Tobias, Courtney R. Brooks, Jonathan H. Teichroeb, Dean H. Betts
    Pages 69-83

  8. Isolation and Culture of Porcine Embryonic Stem Cells

    • Ivan Vassiliev, Mark B. Nottle
    Pages 85-95

  9. Isolation and Culture of Pig Epiblast Stem Cells

    • Aida Rodriguez, David A. Contreras, Ramiro Alberio
    Pages 97-110

  10. Isolation and Culture of Bovine Embryonic Stem Cells

    • Shanbo Cao, Fang Wang, Lin Liu
    Pages 111-123

  11. Analysis of Molecular Markers for Staging Peri-gastrulating Bovine Embryos

    • Isabelle Hue, Severine A. Degrelle, Christoph Viebahn
    Pages 125-135

  12. Chicken Embryonic Stem Cells: Establishment and Characterization

    • Pauline Aubel, Bertrand Pain
    Pages 137-150

  13. Manipulating the Avian Epiblast and Epiblast-Derived Stem Cells

    • Cantas Alev, Mikiharu Nakano, Yuping Wu, Hiroyuki Horiuchi, Guojun Sheng
    Pages 151-173

  14. Stepwise Differentiation from Naïve State Pluripotent Stem Cells to Functional Primordial Germ Cells Through an Epiblast-Like State

    • Katsuhiko Hayashi, Mitinori Saitou
    Pages 175-183

  15. Isolation and Culture of Porcine Neural Progenitor Cells from Embryos and Pluripotent Stem Cells

    • Mikkel A. Rasmussen, Vanessa J. Hall, Poul Hyttel
    Pages 185-198

  16. Derivation of Neural Precursors from Bovine Preimplantation Embryos

    • Giovanna Lazzari, Silvia Colleoni, Cesare Galli
    Pages 199-207

  17. Back Matter

    Pages 209-216
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Keywords

About this book

In Epiblast Stem Cells: Methods and Protocols, expect researchers in the field provide a detailed collection of techniques and protocols useful to the study of the biology of the pluripotent epiblast.  These include methods and techniques used to study epiblast development in different amniotes. This collection brings together contributions from the fields of embryology, stem cell biology and developmental biology together, providing a single volume with detailed procedures for the isolation and culture of epiblasts at different stages of development, and techniques for the study of differentiation into specific lineages. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, a complete list of the necessary materials and reagents, detailed laboratory protocols, and extensive notes providing suggestions on troubleshooting and how to overcome common difficulties.

 

Comprehensive and cutting-edge, Epiblast Stem Cells: Methods and Protocols serves as a resource to individuals interested in studying the biology of pluripotent cells.

Editors and Affiliations

  • School of Biosciences Rm 223 South Lab, University of Nottingham, Loughborough, United Kingdom

    Ramiro Alberio

Bibliographic Information

  • Book Title: Epiblast Stem Cells

  • Book Subtitle: Methods and Protocols

  • Editors: Ramiro Alberio

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-628-3

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, New York 2013

  • Hardcover ISBN: 978-1-62703-627-6Published: 23 August 2013

  • Softcover ISBN: 978-1-4939-6033-0Published: 23 August 2016

  • eBook ISBN: 978-1-62703-628-3Published: 23 August 2013

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 216

  • Number of Illustrations: 15 b/w illustrations, 34 illustrations in colour

  • Topics: Cell Biology, Stem Cells

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