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Fluorescent Protein-Based Biosensors

Methods and Protocols

  • Book
  • © 2014

Overview

Editors:
  1. Jin Zhang

    1. Dept. of Pharmacology & Molecular Scienc, Johns Hopkins University, Baltimore, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. Qiang Ni

    1. Dept. of Pharmacology & Molecular Scienc, Johns Hopkins University, Baltimore, USA

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    You can also search for this editor in PubMed   Google Scholar

  3. Robert H Newman

    1. Department of Biology, North Caroline A&T State Univ, Greensboro, USA

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1071)

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Table of contents (18 protocols)

  1. Front Matter

    Pages i-xi
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  2. The Design and Application of Genetically Encodable Biosensors Based on Fluorescent Proteins

    • Robert H. Newman, Jin Zhang
    Pages 1-16

  3. An Introduction to Fluorescence Imaging Techniques Geared Towards Biosensor Applications

    • J. Goedhart, Mark A. Hink, Kees Jalink
    Pages 17-28

  4. Quantitative Measurement of Ca2+ and Zn2+ in Mammalian Cells Using Genetically Encoded Fluorescent Biosensors

    • J. Genevieve Park, Amy E. Palmer
    Pages 29-47

  5. Detecting cAMP with an Epac-Based FRET Sensor in Single Living Cells

    • J. Klarenbeek, Kees Jalink
    Pages 49-58

  6. Analysis of Compartmentalized cAMP: A Method to Compare Signals from Differently Targeted FRET Reporters

    • Alessandra Stangherlin, Andreas Koschinski, Anna Terrin, Anna Zoccarato, He Jiang, Laura Ashley Fields et al.
    Pages 59-71

  7. Genetically Encoded Fluorescent Biosensors for Live Cell Imaging of Lipid Dynamics

    • Moritoshi Sato
    Pages 73-81

  8. Live-Cell Imaging of Cytosolic NADH–NAD+ Redox State Using a Genetically Encoded Fluorescent Biosensor

    • Yin Pun Hung, Gary Yellen
    Pages 83-95

  9. Measuring Membrane Voltage with Microbial Rhodopsins

    • Adam E. Cohen, Daniel R. Hochbaum
    Pages 97-108

  10. Imaging the Activity of Ras Superfamily GTPase Proteins in Small Subcellular Compartments in Neurons

    • Ana F. Oliveira, Ryohei Yasuda
    Pages 109-128

  11. Imaging Kinase Activity at Protein Scaffolds

    • Maya T. Kunkel, Alexandra C. Newton
    Pages 129-137

  12. Using a Genetically Encoded FRET-Based Reporter to Visualize Calcineurin Phosphatase Activity in Living Cells

    • Sohum Mehta, Jin Zhang
    Pages 139-149

  13. Genetically Encoded FRET Indicators for Live-Cell Imaging of Histone Acetylation

    • Kazuki Sasaki, Minoru Yoshida
    Pages 151-161

  14. Genetically Encoded Fluorescent Biosensors for Live-Cell Imaging of MT1-MMP Protease Activity

    • Mingxing Ouyang, Shaoying Lu, Yingxiao Wang
    Pages 163-174

  15. Biosensor Imaging in Brain Slice Preparations

    • Marina Polito, Pierre Vincent, Elvire Guiot
    Pages 175-194

  16. Optical Calcium Imaging Using DNA-Encoded Fluorescence Sensors in Transgenic Fruit Flies, Drosophila melanogaster

    • Shubham Dipt, Thomas Riemensperger, André Fiala
    Pages 195-206

  17. A Multiparameter Live Cell Imaging Approach to Monitor Cyclic AMP and Protein Kinase A Dynamics in Parallel

    • Nwe-Nwe Aye-Han, Jin Zhang
    Pages 207-215

  18. FRET and BRET-Based Biosensors in Live Cell Compound Screens

    • Katie Herbst Robinson, Jessica R. Yang, Jin Zhang
    Pages 217-225

  19. Integrating Fluorescent Biosensor Data Using Computational Models

    • Eric C. Greenwald, Renata K. Polanowska-Grabowska, Jeffrey J. Saucerman
    Pages 227-248

  20. Back Matter

    Pages 249-251
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Keywords

About this book

In Fluorescent Protein-Based Biosensors: Methods and Protocols, experts in the field have assembled a series of protocols describing several methods in which fluorescent protein-based reporters can be used to gain unique insights into the regulation of cellular signal transduction.  Genetically encodable fluorescent biosensors have allowed researchers to observe biochemical processes within the endogenous cellular environment with unprecedented spatiotemporal resolution. As the number and diversity of available biosensors grows, it is increasingly important to equip researchers with an understanding of the key concepts underlying the design and application of genetically encodable fluorescent biosensors to live cell imaging. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, Fluorescent Protein-Based Biosensors: Methods and Protocols promises to be a valuable resource for researchers interested in applying current biosensors to the study of biochemical processes in living cells as well as those interested in developing novel biosensors to visualize other cellular phenomena.

Editors and Affiliations

  • Dept. of Pharmacology & Molecular Scienc, Johns Hopkins University, Baltimore, USA

    Jin Zhang, Qiang Ni

  • Department of Biology, North Caroline A&T State Univ, Greensboro, USA

    Robert H Newman

Bibliographic Information

  • Book Title: Fluorescent Protein-Based Biosensors

  • Book Subtitle: Methods and Protocols

  • Editors: Jin Zhang, Qiang Ni, Robert H Newman

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-622-1

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2014

  • Hardcover ISBN: 978-1-62703-621-4Published: 20 September 2013

  • Softcover ISBN: 978-1-4939-6014-9Published: 23 August 2016

  • eBook ISBN: 978-1-62703-622-1Published: 20 September 2013

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 251

  • Number of Illustrations: 12 b/w illustrations, 48 illustrations in colour

  • Topics: Protein Science, Cell Physiology

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