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Stem Cell Nanotechnology

Methods and Protocols

  • Book
  • © 2013

Overview

Editors:
  1. Kursad Turksen

    1. Regenerative Medicine Program, Ottawa Hospital Research Institute Sprott Centre for Stem Cell Research, Ottawa, Canada

    View editor publications

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  • Includes cutting-edge methods and protocols for nanotechnological approaches to stem cell biology
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1058)

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Table of contents (13 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Propagation and Differentiation of Human Wharton’s Jelly Stem Cells on Three-Dimensional Nanofibrous Scaffolds

    • Kalamegam Gauthaman, Chui-Yee Fong, Jayarama Reddy Venugopal, Arijit Biswas, Seeram Ramakrishna, Ariff Bongso
    Pages 1-23

  3. Composite Electrospun Nanofibers for Influencing Stem Cell Fate

    • Alessandro Polini, Silvia Scaglione, Rodolfo Quarto, Dario Pisignano
    Pages 25-40

  4. In Vitro Nanoparticle-Mediated Intracellular Delivery into Human Adipose-Derived Stem Cells

    • Rosario Sanchez-Martin, Victoria Cano-Cortés, Juan Antonio Marchal, Macarena Perán
    Pages 41-47

  5. Screening of Nanoparticle Embryotoxicity Using Embryonic Stem Cells

    • Luisa Campagnolo, Ivana Fenoglio, Micol Massimiani, Andrea Magrini, Antonio Pietroiusti
    Pages 49-60

  6. Peptide Nanofiber Scaffolds for Multipotent Stromal Cell Culturing

    • Seher Ustun, Samet Kocabey, Mustafa O. Guler, Ayse B. Tekinay
    Pages 61-76

  7. Patterned Polymeric Surfaces to Study the Influence of Nanotopography on the Growth and Differentiation of Mesenchymal Stem Cells

    • Cristian Pablo Pennisi, Vladimir Zachar, Trine Fink, Leonid Gurevich, Peter Fojan
    Pages 77-88

  8. Transduction of Murine Embryonic Stem Cells by Magnetic Nanoparticle-Assisted Lentiviral Gene Transfer

    • Sarah Rieck, Katrin Zimmermann, Daniela Wenzel
    Pages 89-96

  9. Lanthanide-Based Upconversion Nanoparticles for Connexin-Targeted Imaging in Co-cultures

    • Sounderya Nagarajan, Yong Zhang
    Pages 97-107

  10. Basic Protocols to Investigate hMSC Behavior onto Electrospun Fibers

    • Marco A. Alvarez-Perez, Vincenzo Guarino, Valentina Cirillo, Luigi Ambrosio
    Pages 109-117

  11. General Protocol for the Culture of Cells on Plasma-Coated Electrospun Scaffolds

    • A. Géraldine Guex, Giuseppino Fortunato, Dirk Hegemann, Hendrik T. Tevaearai, Marie-Noëlle Giraud
    Pages 119-131

  12. Mesenchymal Stem Cells and Nano-structured Surfaces

    • Yinghong Zhou, Nishant Chakravorty, Yin Xiao, Wenyi Gu
    Pages 133-148

  13. Chondrogenic Differentiation of Menstrual Blood-Derived Stem Cells on Nanofibrous Scaffolds

    • Somaieh Kazemnejad, Amir-Hassan Zarnani, Manijeh Khanmohammadi, Sahba Mobini
    Pages 149-169

  14. Engineering of a 3D Nanostructured Scaffold Made of Functionalized Self-Assembling Peptides and Encapsulated Neural Stem Cells

    • Carla Cunha, Silvia Panseri, Fabrizio Gelain
    Pages 171-182

  15. Back Matter

    Pages 183-184
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Keywords

About this book

Stem Cell Nanotechnology: Methods and Protocols gathers several representative protocols related to the emerging interest in nanotechnology as it relates to stem cell biology. The detailed chapters presented within have been validated for reproducibility and are described in an easy to follow, step-by-step fashion so as to be valuable for not only experts but also novices in the stem cell field.  As with other volumes in the highly successful Methods in Molecular Biology series, chapters conclude with a Notes section, which provides tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Stem Cell Nanotechnology: Methods and Protocols provides both a flavor of the field as it currently is and a source to stimulate new approaches and methodologies by those interested in nanotechnological applications.

Editors and Affiliations

  • Regenerative Medicine Program, Ottawa Hospital Research Institute Sprott Centre for Stem Cell Research, Ottawa, Canada

    Kursad Turksen

Bibliographic Information

  • Book Title: Stem Cell Nanotechnology

  • Book Subtitle: Methods and Protocols

  • Editors: Kursad Turksen

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-571-2

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2013

  • Hardcover ISBN: 978-1-62703-570-5Published: 13 August 2013

  • Softcover ISBN: 978-1-4939-6327-0Published: 30 April 2017

  • eBook ISBN: 978-1-62703-571-2Published: 15 August 2013

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 184

  • Number of Illustrations: 39 b/w illustrations, 11 illustrations in colour

  • Topics: Cell Biology, Stem Cells

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