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Cyclic Nucleotide Signaling in Plants

Methods and Protocols

  • Book
  • © 2013

Overview

Editors:
  1. Chris Gehring

    1. , Division of Chemical and Life Sciences a, 4700 King Abdullah University of Science, Thuwal, Saudi Arabia

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  • Includes cutting-edge methods and protocols in the MiMB standard format
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1016)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. Molecular Methods for the Study of Signal Transduction in Plants

    • Helen R. Irving, Chris Gehring
    Pages 1-11

  3. Recombinant Expression and Functional Testing of Candidate Adenylate Cyclase Domains

    • Oziniel Ruzvidzo, Bridget T. Dikobe, David T. Kawadza, Grace H. Mabadahanye, Patience Chatukuta, Lusisizwe Kwezi
    Pages 13-25

  4. Quantification of Cyclic Dinucleotides by Reversed-Phase LC-MS/MS

    • Heike Burhenne, Volkhard Kaever
    Pages 27-37

  5. Determination of ADP-Ribosyl Cyclase Activity, Cyclic ADP-Ribose, and Nicotinic Acid Adenine Dinucleotide Phosphate in Tissue Extracts

    • Richard M. Graeff, Hon Cheung Lee
    Pages 39-56

  6. In Vivo Imaging of cGMP in Plants

    • Jean-Charles Isner, Frans J. M. Maathuis
    Pages 57-65

  7. Characterization of Heterologously Expressed Transporter Genes by Patch- and Voltage-Clamp Methods: Application to Cyclic Nucleotide-Dependent Responses

    • Fouad Lemtiri-Chlieh, Rashid Ali
    Pages 67-93

  8. Noninvasive Microelectrode Ion Flux Estimation Technique (MIFE) for the Study of the Regulation of Root Membrane Transport by Cyclic Nucleotides

    • Natalia Maria Ordoñez, Lana Shabala, Chris Gehring, Sergey Shabala
    Pages 95-106

  9. Calcium Imaging of the Cyclic Nucleotide Response

    • Martin R. McAinsh, Stephen K. Roberts, Lyudmila V. Dubovskaya
    Pages 107-119

  10. Identification and Quantitation of Signal Molecule-Dependent Protein Phosphorylation

    • Arnoud Groen, Ludivine Thomas, Kathryn Lilley, Claudius Marondedze
    Pages 121-137

  11. Comparative Gel-Based Phosphoproteomics in Response to Signaling Molecules

    • Claudius Marondedze, Kathryn Lilley, Ludivine Thomas
    Pages 139-154

  12. An Affinity Pull-Down Approach to Identify the Plant Cyclic Nucleotide Interactome

    • Lara Donaldson, Stuart Meier
    Pages 155-173

  13. Structural and Functional Characterization of Receptor Kinases with Nucleotide Cyclase Activity

    • Victor Muleya, Janet I. Wheeler, Helen R. Irving
    Pages 175-194

  14. Computational Identification of Candidate Nucleotide Cyclases in Higher Plants

    • Aloysius Wong, Chris Gehring
    Pages 195-205

  15. Identification of Cyclic Nucleotide Gated Channels Using Regular Expressions

    • Alice K. Zelman, Adam Dawe, Gerald A. Berkowitz
    Pages 207-224

  16. Inferring Biological Functions of Guanylyl Cyclases with Computational Methods

    • May Alqurashi, Stuart Meier
    Pages 225-234

  17. Identification and Characterization of Cyclic Nucleotide Phosphodiesterases

    • Erin B. Purcell, Rita Tamayo
    Pages 235-243

  18. Detection of Reactive Oxygen Species Downstream of Cyclic Nucleotide Signals in Plants

    • Robin K. Walker, Gerald A. Berkowitz
    Pages 245-252

  19. Measurement of Nitric Oxide in Plant Tissue Using Difluorofluorescein and Oxyhemoglobin

    • Ndiko Ludidi
    Pages 253-259

  20. Infrared Gas Analysis Technique for the Study of the Regulation of Photosynthetic Responses

    • Alex Valentine, Oziniel Ruzvidzo, Aleysia Kleinert, Yun Kang, Vagner Bennedito
    Pages 261-269
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Keywords

About this book

Over the last two decades there has been a growing interest in cyclic nucleotide research in plants with an emphasis on the elucidation of the roles of cGMP and cAMP. In Cyclic Nucleotide Signaling in Plants: Methods and Protocols, expert researchers in the field detail many approaches to better understand the biological role of this important signaling system. Written in the highly successful Methods in Molecular Biologyâ„¢ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

 

Authoritative and practical, Cyclic Nucleotide Signaling in Plants: Methods and Protocols seeks to aid scientist in further understanding signal transduction and the molecular mechanisms underlying cellular signaling.

Editors and Affiliations

  • , Division of Chemical and Life Sciences a, 4700 King Abdullah University of Science, Thuwal, Saudi Arabia

    Chris Gehring

Bibliographic Information

  • Book Title: Cyclic Nucleotide Signaling in Plants

  • Book Subtitle: Methods and Protocols

  • Editors: Chris Gehring

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-441-8

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2013

  • Hardcover ISBN: 978-1-62703-440-1Published: 17 May 2013

  • Softcover ISBN: 978-1-4939-6291-4Published: 23 August 2016

  • eBook ISBN: 978-1-62703-441-8Published: 17 May 2013

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 273

  • Number of Illustrations: 16 b/w illustrations, 25 illustrations in colour

  • Topics: Plant Sciences, Plant Biochemistry

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