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Necrosis

Methods and Protocols

  • Book
  • © 2013

Overview

Editors:
  1. Kimberly McCall

    1. Department of Biology, Boston University, Boston, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Charles Klein

    1. Brookline, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes a wide range of techniques for the study of necrosis in vitro and in vivo
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1004)

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Table of contents (18 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Analysis of Necrosis In Vitro

    1. Front Matter

      Pages 1-1
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    2. Navigation to the Graveyard-Induction of Various Pathways of Necrosis and Their Classification by Flow Cytometry

      • Christina Janko, Luis Munoz, Ricardo Chaurio, Christian Maueröder, Christian Berens, Kirsten Lauber et al.
      Pages 3-15

    3. Time-Lapse Imaging of Necrosis

      • Fredrik Wållberg, Tencho Tenev, Pascal Meier
      Pages 17-29

    4. Activity Assays for Receptor-Interacting Protein Kinase 1:A Key Regulator of Necroptosis

      • Jenny L. Maki, Alexei Degterev
      Pages 31-42

    5. Fluorescent Biosensors for the Detection of HMGB1 Release

      • Isabelle Martins, Oliver Kepp, Laurie Menger, Mickäel Michaud, Sandy Adjemian, Abdul Qader Sukkurwala et al.
      Pages 43-56

  3. Alternative Cell Death Modalities

    1. Front Matter

      Pages 57-57
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    2. Methods for the Study of Entosis

      • Qiang Sun, Michael Overholtzer
      Pages 59-66

    3. Analysis of Pyroptosis in Bacterial Infection

      • Lia Danelishvili, Luiz E. Bermudez
      Pages 67-73

  4. Analysis of Cell Death in Mammalian Tissues

    1. Front Matter

      Pages 75-75
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    2. Analysis of Cell Death by Electron Microscopy

      • S. Burattini, E. Falcieri
      Pages 77-89

    3. Histological Analysis of Neurodegeneration in the Mouse Brain

      • Hiroo Yamaguchi, Jie Shen
      Pages 91-113

    4. Quantitation of Acute Necrosis After Experimental Myocardial Infarction

      • Xin-Yi Yeap, Shirley Dehn, Jeremy Adelman, Jeremy Lipsitz, Edward B. Thorp
      Pages 115-133

    5. Immunohistological Tools to Discriminate Apoptotic and Necrotic Cell Death in the Skin

      • Marion C. Bonnet
      Pages 135-142

  5. Cell Death in Nonmammalian Model Systems

    1. Front Matter

      Pages 143-143
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    2. Identification and Characterization of Programmed Cell Death Markers in Bacterial Models

      • Daniel J. Dwyer, Jonathan A. Winkler
      Pages 145-159

    3. Quantification of Genetically Controlled Cell Death in Budding Yeast

      • Xinchen Teng, J. Marie Hardwick
      Pages 161-170

    4. Necrosis in C. elegans

      • Matt Crook, Avni Upadhyay, Wendy Hanna-Rose
      Pages 171-182

    5. Monitoring the Clearance of Apoptotic and Necrotic Cells in the Nematode Caenorhabditis elegans

      • Zao Li, Nan Lu, Xiangwei He, Zheng Zhou
      Pages 183-202

    6. Modeling Calcium-Overload Mediated Necrosis in Drosophila

      • Kai Liu, Yuhong Li, Lei Liu
      Pages 203-213
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Keywords

About this book

Cell death is an essential process in development, and a major contributor to a wide range of human diseases.  Three major classifications of cell death, apoptosis, autophagic cell death and necrosis, have been described for years, and the existence of many more forms of cell death is now accepted. In, Necrosis: Methods and Protocols experts in the field provide a wide range of methods and techniques for the study of necrosis in vitro and in vivo. These include methods and techniques for the analysis of necrosis in mammalian cells, characterization of alternative forms of cell death: entosis and pyroptosis, and analysis of cell death in non-mammalian model sytems and mammalian tissues, including chapters on skin, brain, and heart. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
 
 
Authoritative and practical, Necrosis: Methods and Protocols describes techniques  in an easy to follow manner, with details so that beginners can succeed with challenging techniques.

Editors and Affiliations

  • Department of Biology, Boston University, Boston, USA

    Kimberly McCall

  • Brookline, USA

    Charles Klein

Bibliographic Information

  • Book Title: Necrosis

  • Book Subtitle: Methods and Protocols

  • Editors: Kimberly McCall, Charles Klein

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-383-1

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2013

  • Hardcover ISBN: 978-1-62703-382-4Published: 04 June 2013

  • Softcover ISBN: 978-1-4939-6010-1Published: 23 August 2016

  • eBook ISBN: 978-1-62703-383-1Published: 04 June 2013

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 252

  • Number of Illustrations: 51 b/w illustrations

  • Topics: Cell Biology, Cell Cycle Analysis, Apoptosis

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