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Capillary Electrophoresis of Biomolecules

Methods and Protocols

  • Book
  • © 2013

Overview

Editors:
  1. Nicola Volpi

    1. Department of Life Sciences, University of Modena and Reggio Emilia, Modena, Italy

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    You can also search for this editor in PubMed   Google Scholar

  2. Francesca Maccari

    1. , Department of Life Sciences, University of Modena and Reggio Emilia, Modena, Italy

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Focuses on experimental methods for the characterization of carbohydrates, mono-, oligo-, and polysaccharides
  • Presents current, up-to-date methodologies
  • Features tips on troubleshooting and avoiding known pitfalls
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB)

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Table of contents (25 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Recent Advances in Capillary Electrophoresis-Based Proteomic Techniques for Biomarker Discovery

    • Chenchen Wang, Xueping Fang, Cheng S. Lee
    Pages 1-12

  3. Capillary Electrophoresis–Mass Spectrometry of Carbohydrates

    • Joseph Zaia
    Pages 13-25

  4. Approaches to Enhancing the Sensitivity of Carbohydrate Separations in Capillary Electrophoresis

    • M. C. Breadmore
    Pages 27-43

  5. Determination of Monosaccharides Derivatized with 2-Aminobenzoic Acid by Capillary Electrophoresis

    • Mitsuru Abo, Li-ping He, Kae Sato, Akira Okubo
    Pages 45-50

  6. Determination of Mono-, Di-, and Oligosaccharides by Capillary Electrophoresis with Capacitively Coupled Contactless Conductivity Detection

    • Claudimir Lucio do Lago, Thiago Nogueira, Lucas Blanes, Renata Mayumi Saito
    Pages 51-60

  7. Separation of Chitooligosaccharides in Acidic Solution by Capillary Electrophoresis

    • Toshiaki Hattori, Nobuhiro Anraku, Ryo Kato
    Pages 61-66

  8. Capillary Electrophoresis for the Analysis of Glycosaminoglycan-Derived Disaccharides

    • Yuqing Chang, Bo Yang, Amanda Weyers, Robert J. Linhardt
    Pages 67-77

  9. High-Throughput Capillary Electrophoresis–Mass Spectrometry: From Analysis of Amino Acids to Analysis of Protein Complexes

    • Mehdi Moini
    Pages 79-119

  10. Separation of Amino Acids by Capillary Electrophoresis with Light-Emitting Diode-Induced Fluorescence in the Presence of Electroosmotic Flow

    • Ming-Mu Hsieh, Po-Ling Chang
    Pages 121-129

  11. Quantification of Arginine and Dimethylated Arginines in Human Plasma by Field-Amplified Sample Injection Capillary Electrophoresis UV Detection

    • Angelo Zinellu, Salvatore Sotgia, Luca Deiana, Ciriaco Carru
    Pages 131-138

  12. Capillary Electrophoresis-Mass Spectrometry for Peptide Analysis: Target-Based Approaches and Proteomics/Peptidomics Strategies

    • Carolina Simó, Alejandro Cifuentes, Václav Kašička
    Pages 139-151

  13. High-Resolution Proteome/Peptidome Analysis of Body Fluids by Capillary Electrophoresis Coupled with MS

    • Amaya Albalat, Vasiliki Bitsika, Petra Zurbig, Justyna Siwy, William Mullen
    Pages 153-165

  14. Contribution of CE to the Analysis of Protein or Peptide Biomarkers

    • Kiarach Mesbah, Romain Verpillot, François de l’Escaille, Jean Bernard Falmagne, Myriam Taverna
    Pages 167-190

  15. Highly Charged Polyelectrolyte Coatings to Prevent Adsorption During Protein and Peptide Analysis in Capillary Electrophoresis

    • Reine Nehmé, Catherine Perrin
    Pages 191-206

  16. Capillary Electrophoresis with Laser-Induced Fluorescence Detection of Proteins from Two Types of Complex Sample Matrices: Food and Biological Fluids

    • Raul Garrido-Medina, Angel Puerta, Cristina Pelaez-Lorenzo, Zuly Rivera-Monroy, Andras Guttman, Jose Carlos Diez-Masa et al.
    Pages 207-225

  17. Automated Capillary Electrophoresis in the Screening for Hemoglobinopathies

    • Frédéric Cotton, Fleur Wolff, Béatrice Gulbis
    Pages 227-235

  18. Protein Fingerprinting of Staphylococcus aureus by Capillary Electrophoresis with On-Capillary Derivatization and Laser-Induced Fluorescence Detection

    • Cristina Pelaez-Lorenzo, Maria Teresa Veledo, Ramon Gonzalez, Mercedes de Frutos, Jose Carlos Diez-Masa
    Pages 237-251

  19. Capillary Electrophoresis of Seed Storage Proteins: The Separation and Identification of Microheterogeneous Rice Glutelin Subunits

    • Tomoyuki Katsube-Tanaka
    Pages 253-261

  20. Evaluating Amyloid Beta (Aβ) 1-40 Degradation by Capillary Electrophoresis

    • Benjamin J. Alper, Walter K. Schmidt
    Pages 263-273
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Keywords

About this book

Capillary electrophoresis (CE) is a relatively new separation technique suitable for handling small amounts of sample very important in bioanalytical research and in various clinical, diagnostic, genetic, and forensic applications. In Capillary Electrophoresis of Biomolecules: Methods and Protocols, expert researchers in the field provide key techniques to investigate CE focusing on simple and complex carbohydrates (polysaccharides), aminoacids, peptides and proteins, enzymes, and nucleic acids. Along with practical procedures, reviews discussing CE applications related to bio(macro)molecules are also included. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Capillary Electrophoresis of Biomolecules: Methods and Protocols provides the reader with the latest break throughs and improvements in CE and CE techniques applied to several classes of bio(macro)molecules.

Editors and Affiliations

  • Department of Life Sciences, University of Modena and Reggio Emilia, Modena, Italy

    Nicola Volpi

  • , Department of Life Sciences, University of Modena and Reggio Emilia, Modena, Italy

    Francesca Maccari

Bibliographic Information

  • Book Title: Capillary Electrophoresis of Biomolecules

  • Book Subtitle: Methods and Protocols

  • Editors: Nicola Volpi, Francesca Maccari

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-296-4

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2013

  • Hardcover ISBN: 978-1-62703-295-7Published: 06 February 2013

  • Softcover ISBN: 978-1-4939-6248-8Published: 23 August 2016

  • eBook ISBN: 978-1-62703-296-4Published: 11 February 2013

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 358

  • Topics: Biochemistry, general, Electrophoresis

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